Abstract
A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromatography so that crystal lots of greater uniformity could be produced. Significantly more of toxin in the starting culture was recovered as crystals. The lots of crystals obtained initially by either the original or the modified method contained an antigen that was eliminated when the lots were recrystallized.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boroff D. A., Fleck U. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum. J Bacteriol. 1966 Nov;92(5):1580–1581. doi: 10.1128/jb.92.5.1580-1581.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boroff D. A., Townend R., Fleck U., DasGupta B. R. Ultracentrifugal analysis of the crystalline toxin and isolated fractions of Clostridium botulinum type A. J Biol Chem. 1966 Nov 25;241(22):5165–5167. [PubMed] [Google Scholar]
- Knox J., Brown W. P., Spero L. The role of sulfhydryl groups in the activity of type A botulinum toxin. Biochim Biophys Acta. 1970 Aug 21;214(2):350–354. doi: 10.1016/0005-2795(70)90012-7. [DOI] [PubMed] [Google Scholar]
- LAMANNA C., LOWENTHAL J. P. The lack of identity between hemagglutinin and the toxin of type A botulinal organism. J Bacteriol. 1951 Jun;61(6):751–752. doi: 10.1128/jb.61.6.751-752.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamanna C., Eklund H. W., McElroy O. E. Botulinum Toxin (Type A); Including a Study of Shaking with Chloroform as a Step in the Isolation Procedure. J Bacteriol. 1946 Jul;52(1):1–13. [PMC free article] [PubMed] [Google Scholar]
- Lamanna C., McElroy O. E., Eklund H. W. The Purification and Crystallization of Clostridium botulinum Type A Toxin. Science. 1946 May 17;103(2681):613–614. doi: 10.1126/science.103.2681.613. [DOI] [PubMed] [Google Scholar]
- Sugii S., Sakaguchi G. Molecular construction of Clostridium botulinum type A toxins. Infect Immun. 1975 Dec;12(6):1262–1270. doi: 10.1128/iai.12.6.1262-1270.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiyama H., Das Gupta R., Yang K. H. Disulfide-toxicity relationship of botulinal toxin types A, E, and F. Proc Soc Exp Biol Med. 1973 Jul;143(3):589–591. doi: 10.3181/00379727-143-37372. [DOI] [PubMed] [Google Scholar]
- Sugiyama H., Oishi I., Dasgupta B. R. Evaluation of type A botulinal toxin assays that use antitoxin to crystalline toxin. Appl Microbiol. 1974 Feb;27(2):333–336. doi: 10.1128/am.27.2.333-336.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]