Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1977 May;33(5):1149–1155. doi: 10.1128/aem.33.5.1149-1155.1977

Evaluation of a new presumptive medium for group D streptococci.

R L Abshire
PMCID: PMC170841  PMID: 406842

Abstract

A new medium designated as D streptococcus-enterococcus broth was formulated and evaluated for the enrichment and isolation of strains of serological group D streptococci. This medium was made by modifying Todd-Hewitt broth. Most-probable-number multiple-tube and membrane filter techniques were employed to estimate the numbers of enterococci in known cultures, wastewater, and other samples. Preliminary most-probable-number counts with this medium were as much as 3 logs higher than those counts obtained from four other media with which it was compared. The methodology for using this medium to estimate the numbers of group D streptococci in water is discussed.

Full text

PDF
1149

Images in this article

1153Image
on p.1153

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DEIBEL R. H., NIVEN C. F., Jr PYRUVATE FERMENTATION BY STREPTOCOCCUS FAECALIS. J Bacteriol. 1964 Jul;88:4–10. doi: 10.1128/jb.88.1.4-10.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DEIBEL R. H. THE GROUP D STREPTOCOCCI. Bacteriol Rev. 1964 Sep;28:330–366. doi: 10.1128/br.28.3.330-366.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Facklam R. R. Comparison of several laboratory media for presumptive identification of enterococci and group D streptococci. Appl Microbiol. 1973 Aug;26(2):138–145. doi: 10.1128/am.26.2.138-145.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R., Moody M. D. Presumptive identification of group D streptococci: the bile-esculin test. Appl Microbiol. 1970 Aug;20(2):245–250. doi: 10.1128/am.20.2.245-250.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. doi: 10.1128/am.23.6.1131-1139.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GERENCSER V. F., WEAVER R. H. A new technique for the use of sodium azide (hydrazoic acid) as an inhibitive agent. Appl Microbiol. 1959 Mar;7(2):113–115. doi: 10.1128/am.7.2.113-115.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gross K. C., Houghton M. P., Senterfit L. B. Presumptive speciation of Streptococcus bovis and other group D streptococci from human sources by using arginine and pyruvate tests. J Clin Microbiol. 1975 Jan;1(1):54–60. doi: 10.1128/jcm.1.1.54-60.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hajna A. A., Perry C. A. Comparative Study of Presumptive and Confirmative Media for Bacteria of the Coliform Group and for Fecal Streptococci. Am J Public Health Nations Health. 1943 May;33(5):550–556. doi: 10.2105/ajph.33.5.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartman P. A., Reinbold G. W., Saraswat D. S. Media and methods for isolation and enumeration of the enterococci. Adv Appl Microbiol. 1966;8:253–289. [PubMed] [Google Scholar]
  11. KENNER B. A., CLARK H. F., KABLER P. W. Fecal Streptococci. I. Cultivation and enumeration of Streptococci in surface waters. Appl Microbiol. 1961 Jan;9:15–20. doi: 10.1128/am.9.1.15-20.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LITSKY W., MALLMANN W. L., FIFIELD C. W. A new medium for the detection of enterococci in water. Am J Public Health Nations Health. 1953 Jul;43(7):873–879. doi: 10.2105/ajph.43.7.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levin M. A., Fischer J. R., Cabelli V. J. Membrane filter technique for enumeration of enterococci in marine waters. Appl Microbiol. 1975 Jul;30(1):66–71. doi: 10.1128/am.30.1.66-71.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MALLMANN W. L., SELIGMANN E. B., Jr A comparative study of media for the detection of streptococci in water and sewage. Am J Public Health Nations Health. 1950 Mar;40(3):286–289. doi: 10.2105/ajph.40.3.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MEDREK T. F., BARNES E. M. The influence of the growth medium on the demonstration of a group D antigen in faecal streptococci. J Gen Microbiol. 1962 Sep;28:701–709. doi: 10.1099/00221287-28-4-701. [DOI] [PubMed] [Google Scholar]
  16. Medrek T. F., Litsky W. Comparative Incidence of Coliform Bacteria and Enterococci in Undisturbed Soil. Appl Microbiol. 1960 Jan;8(1):60–63. doi: 10.1128/am.8.1.60-63.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mundt J. O., Graham W. F. Streptococcus faecium var. casselifavus, nov. var. J Bacteriol. 1968 Jun;95(6):2005–2009. doi: 10.1128/jb.95.6.2005-2009.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
  19. SLANETZ L. W., BARTLEY C. H. Numbers of enterococci in water, sewage, and feces determined by the membrane filter technique with an improved medium. J Bacteriol. 1957 Nov;74(5):591–595. doi: 10.1128/jb.74.5.591-595.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SLANETZ L. W., BARTLEY C. H. SURVIVAL OF FECAL STREPTOCCOCCI IN SEA WATER. Health Lab Sci. 1965 Jul;2:142–148. [PubMed] [Google Scholar]
  21. Slanetz L. W., Bartley C. H., Stanley K. W. Coliforms, fecal streptococci and Salmonella in seawater and shellfish. Health Lab Sci. 1968 Apr;5(2):66–78. [PubMed] [Google Scholar]
  22. Splittstoesser D. F., Wright R., Hucker G. J. Studies on Media for Enumerating Enterococci in Frozen Vegetables. Appl Microbiol. 1961 Jul;9(4):303–308. doi: 10.1128/am.9.4.303-308.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES