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. 1977 May;33(5):1215–1217. doi: 10.1128/aem.33.5.1215-1217.1977

Gentle Lysis of Staphylococcus aureus at Low Temperature

Russell S Flowers 1, Scott E Martin 1, Z John Ordal 1
PMCID: PMC170852  PMID: 327931

Abstract

A low-temperature lysis procedure for Staphylococcus aureus is described. It is simple and requires no special equipment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bluhm L., Ordal Z. J. Effect of sublethal heat on the metabolic activity of Staphylococcus aureus. J Bacteriol. 1969 Jan;97(1):140–150. doi: 10.1128/jb.97.1.140-150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Body B. A., Brownstein B. H. Ribosomal precursor particles of Bacillus megaterium. J Bacteriol. 1976 Jun;126(3):1149–1155. doi: 10.1128/jb.126.3.1149-1155.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Godson G. N., Sinsheimer R. L. Lysis of Escherichia coli with a neutral detergent. Biochim Biophys Acta. 1967 Dec 19;149(2):476–488. doi: 10.1016/0005-2787(67)90175-x. [DOI] [PubMed] [Google Scholar]
  4. Gomez R. F., Sinskey A. J. Deoxyribonucleic acid breaks in heated Salmonella typhimurium LT-2 after exposure to nutritionally complex media. J Bacteriol. 1973 Aug;115(2):522–528. doi: 10.1128/jb.115.2.522-528.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ron E. Z., Kohler R. E., Davis B. D. Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis. Science. 1966 Sep 2;153(3740):1119–1120. doi: 10.1126/science.153.3740.1119. [DOI] [PubMed] [Google Scholar]
  6. SCHINDLER C. A., SCHUHARDT V. T. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proc Natl Acad Sci U S A. 1964 Mar;51:414–421. doi: 10.1073/pnas.51.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]

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