Abstract
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference between the two techniques when Nuclepore filters were used (0.5 less than P less than 0.9). The average cell volume from this study area was 0.047 micron3. The estimated number of bacteria ranged from 10(6) to 10(7) bacteria per ml, representing from 4 to 40 mg of C per m3.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fleischer R. L., Price P. B., Symes E. M. Novel Filter for Biological Materials. Science. 1964 Jan 17;143(3603):249–250. doi: 10.1126/science.143.3603.249. [DOI] [PubMed] [Google Scholar]
- Gray T. R. Stereoscan electron microscopy of soil microorganisms. Science. 1967 Mar 31;155(3770):1668–1670. doi: 10.1126/science.155.3770.1668. [DOI] [PubMed] [Google Scholar]
- Hagen C. A., Hawrylewicz E. J., Anderson B. T., Tolkacz V. K., Cephus M. L. Use of the scanning electron microscope for viewing bacteria in soil. Appl Microbiol. 1968 Jun;16(6):932–934. doi: 10.1128/am.16.6.932-934.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurtzman C. P., Baker F. L., Smiley M. J. Specimen holder to critical-point dry microorganisms for scanning electron microscopy. Appl Microbiol. 1974 Oct;28(4):708–712. doi: 10.1128/am.28.4.708-712.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todd R. L., Kerr T. J. Scanning electron microscopy of microbial cells on membrane filters. Appl Microbiol. 1972 Jun;23(6):1160–1162. doi: 10.1128/am.23.6.1160-1162.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watson S. W., Novitsky T. J., Quinby H. L., Valois F. W. Determination of bacterial number and biomass in the marine environment. Appl Environ Microbiol. 1977 Apr;33(4):940–946. doi: 10.1128/aem.33.4.940-946.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]