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. 1981 Jan;37(1):17–23. doi: 10.1128/jvi.37.1.17-23.1981

Biochemical, biophysical, and biological properties of densonucleosis virus (parvovirus). III. common sequences of structural proteins.

P Tijssen, E Kurstak
PMCID: PMC170977  PMID: 7218424

Abstract

Densonucleosis virus cannot code for its four structural proteins if each of them has a unique sequence. The objective of the present investigation, therefore, was to establish whether: (i) the viral genome contains overlapping genes; (ii) the virus incorporates host proteins; or (iii) one of the structural proteins is a dimer. Two independent methods were employed for this purpose. First, the viral proteins, solubilized in sodium dodecyl sulfate, were purified after dansylation and were analyzed by peptide mapping, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Second, an enzyme-linked immunosorbent assay was developed for a comparative analysis of the viral proteins solubilized by sodium dodecyl sulfate. It was demonstrated with both techniques that densonucleosis virus has four unique structural proteins, all with extensive sequence homologies. Moreover, all structural proteins contained intraprotein, but no interprotein, disulfide linkages. These results indicated similarities between densonucleosis virus and representatives of the two other genera of the Parvoviridae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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