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. 1981 Jan;37(1):317–327. doi: 10.1128/jvi.37.1.317-327.1981

Noncytopathic mutants of Newcastle disease virus are defective in virus-specific RNA synthesis.

C H Madansky, M A Bratt
PMCID: PMC171009  PMID: 7218426

Abstract

We have studied virus-specific RNA synthesis in cells infected by six noncytopathic (nc) mutants of the Australia-Victoria wild-type strain (AV-WT) of Newcastle disease virus (NDV) (19). The rates of NDV-specific RNA synthesis in mutant infection were three to sevenfold lower than those observed in wild-type infection. Velocity sedimentation of this NDV-specific RNA revealed that the lower rates of synthesis in mutant infection correlated with reduced accumulation of 18S and 35S mRNA. Electrophoresis in polyacrylamide-urea gels showed that accumulation of all of the 18S mRNA species was reduced and no new species could be detected. Primary transcription appeared unaltered in mutant infection. Cells infected with two naturally occurring avirulent strains of NDV also showed less accumulation of 18S mRNA. Electrophoresis of this RNA resulted in patterns which differed from those obtained with RNA from either AV-WT or nc mutant infection. Complementation for RNA accumulation between the nc mutants and RNA- temperature-sensitive mutants of AV-WT (32) suggested a common defect in the nc mutants. Analysis of plaque-forming revertants of five of the nc mutants revealed that viral RNA synthetic capacity, cell killing, and plaque-forming ability correlated absolutely. These results suggest that viral RNA synthesis and cytopathogenicity may be causally related. In addition, several of the plaque-forming (and cell-killing) revertants were found to be unable to induce fusion from within in infected cell cultures. This result, coupled with the finding that several of the nc mutants are capable of wild-type levels of fusion from within, suggests that the ability to cause such fusion does not correlate with the ability to kill cells.

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Selected References

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  1. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  2. Bratt M. A., Gallaher W. R. Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):536–543. doi: 10.1073/pnas.64.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clavell L. A., Bratt M. A. Relationship between the ribonucleic acid synthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. J Virol. 1971 Oct;8(4):500–508. doi: 10.1128/jvi.8.4.500-508.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clinkscales C. W., Bratt M. A., Morrison T. G. Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system. J Virol. 1977 Apr;22(1):97–101. doi: 10.1128/jvi.22.1.97-101.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins B. S., Bratt M. A. Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2544–2548. doi: 10.1073/pnas.70.9.2544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins P. L., Hightower L. E., Ball L. A. Transcription and translation of Newcastle disease virus mRNA's in vitro. J Virol. 1978 Oct;28(1):324–336. doi: 10.1128/jvi.28.1.324-336.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Colonno R. J., Stone H. O. Isolation of a transcriptive complex from Newcastle disease virions. J Virol. 1976 Sep;19(3):1080–1089. doi: 10.1128/jvi.19.3.1080-1089.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Floyd R. W., Stone M. P., Joklik W. K. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Anal Biochem. 1974 Jun;59(2):599–609. doi: 10.1016/0003-2697(74)90313-3. [DOI] [PubMed] [Google Scholar]
  9. Graves M. C., Silver S. M., Choppin P. W. Measles virus polypeptides synthesis in infected cells. Virology. 1978 May 1;86(1):254–263. doi: 10.1016/0042-6822(78)90025-9. [DOI] [PubMed] [Google Scholar]
  10. Hightower L. E., Bratt M. A. Protein synthesis in Newcastle disease virus-infected chicken embryo cells. J Virol. 1974 Apr;13(4):788–800. doi: 10.1128/jvi.13.4.788-800.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holmes K. V., Choppin P. W. On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types. J Exp Med. 1966 Sep 1;124(3):501–520. doi: 10.1084/jem.124.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hunt D. M., Emerson S. U., Wagner R. R. RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants. J Virol. 1976 May;18(2):596–603. doi: 10.1128/jvi.18.2.596-603.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lomniczi B., Meager A., Burke D. C. Virus RNA and protein synthesis in cells infected with different strains of Newcastle disease virus. J Gen Virol. 1971 Oct;13(1):111–120. doi: 10.1099/0022-1317-13-1-111. [DOI] [PubMed] [Google Scholar]
  15. Madansky C. H., Bratt M. A. Noncytopathic mutants of Newcastle disease virus. J Virol. 1978 Jun;26(3):724–729. doi: 10.1128/jvi.26.3.724-729.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marcus P. I., Sekellick M. J. Cell killing by viruses. II. Cell killing by vesicular stomatitis virus: a requirement for virion-derived transcription. Virology. 1975 Jan;63(1):176–190. doi: 10.1016/0042-6822(75)90383-9. [DOI] [PubMed] [Google Scholar]
  17. McGeoch D., Fellner P., Newton C. Influenza virus genome consists of eight distinct RNA species. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045–3049. doi: 10.1073/pnas.73.9.3045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nagai Y., Klenk H. D., Rott R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 1976 Jul 15;72(2):494–508. doi: 10.1016/0042-6822(76)90178-1. [DOI] [PubMed] [Google Scholar]
  19. Portner A., Scroggs R. A., Marx P. S., Kingsbury D. W. A temperature-sensitive mutant of Sendai virus with an altered hemagglutinin-neuraminidase polypeptide: consequences for virus assembly and cytopathology. Virology. 1975 Sep;67(1):179–187. doi: 10.1016/0042-6822(75)90415-8. [DOI] [PubMed] [Google Scholar]
  20. Preble O. T., Youngner J. S. Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus. J Virol. 1973 Sep;12(3):472–480. doi: 10.1128/jvi.12.3.472-480.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sarver N., Stollar V. Sindbis virus-induced cytopathic effect in clones of Aedes albopictus (Singh) cells. Virology. 1977 Jul 15;80(2):390–400. doi: 10.1016/s0042-6822(77)80014-7. [DOI] [PubMed] [Google Scholar]
  22. Scheid A., Choppin P. W. Protease activation mutants of sendai virus. Activation of biological properties by specific proteases. Virology. 1976 Jan;69(1):265–277. doi: 10.1016/0042-6822(76)90213-0. [DOI] [PubMed] [Google Scholar]
  23. Shapiro S. C., Bratt M. S. Proteins of four biologically distinct strains of Newcastle disease virus. Proc Soc Exp Biol Med. 1971 Mar;136(3):834–838. doi: 10.3181/00379727-136-35375. [DOI] [PubMed] [Google Scholar]
  24. Szilágyi J. F., Pringle C. R. Effect of temperature-sensitive mutations on the virion-associated RNA transcriptase of vesicular stomatitis virus. J Mol Biol. 1972 Nov 14;71(2):281–291. doi: 10.1016/0022-2836(72)90351-8. [DOI] [PubMed] [Google Scholar]
  25. Tsipis J. E., Bratt M. A. Isolation and preliminary characterization of temperature-sensitive mutants of Newcastle disease virus. J Virol. 1976 Jun;18(3):848–855. doi: 10.1128/jvi.18.3.848-855.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weiss S. R., Bratt M. A. Comparative electrophoresis of the 18-22S RNAs of Newcastle disease virus. J Virol. 1976 Apr;18(1):316–323. doi: 10.1128/jvi.18.1.316-323.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Winston S. H., Rustigian R., Bratt M. A. Persistent infection of cells in culture by measles virus. 3. Comparison of virus-specific RNA synthesized in primary persistent infection in HeLa cells. J Virol. 1973 Jun;11(6):926–932. doi: 10.1128/jvi.11.6.926-932.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Youngner J. S., Dubovi E. J., Quagliana D. O., Kelly M., Preble O. T. Role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus. J Virol. 1976 Jul;19(1):90–101. doi: 10.1128/jvi.19.1.90-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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