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. 1981 Feb;37(2):654–660. doi: 10.1128/jvi.37.2.654-660.1981

Viral protein expression in producer and nonproducer clones of friend erythroleukemia cell lines.

R Anand, F Lilly, S Ruscetti
PMCID: PMC171053  PMID: 6938735

Abstract

Erythroleukemia cell lines HFL/d and HFL/b, derived from tumors induced in vivo in BALB/c (H-2d) and congenic BALB.B (H-2b) mice, respectively, by a polycythemia-inducing strain of Friend virus, produced both spleen focus-forming virus (SFFV) and its native NB-tropic helper virus (Friend murine leukemia virus [FMuLV]) during early-passage generations in culture. Eventually each line ceased production of both infectious viruses but retained its tumorigenic potential in syngeneic hosts. Virus-producer and -nonproducer clones of these cell lines were examined for expression of proteins encoded by the SFFV or FMuLV genomes. Lysates of labeled cells were treated with various antiviral sera, and the precipitates were examined by gel electrophoresis. Expression of the FMuLV env gene-encoded precursor protein, gPr84env, was observed in all producer and most nonproducer clones, but the FMuLV gag and pol gene products, Pr65gag and Pr200gag-pol, were uniformly undetectable in nonproducer clones. All HFL/d and HFL/b clones expressed appreciable amounts of the SFFV-encoded envelope protein, gp52, including one exceptional clone which had ceased to express any FMuLV-encoded proteins. The molecular weight of this SFFV-encoded envelope protein was consistently smaller in all HFL/b clones than in HFL/d clones, regardless of their producer or nonproducer status. The virus-nonproducer phenotype thus appears to be due to shutdown of expression of the 5' portion of the FMuLV genome in two independent cell lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. A lot of knowledge is dangerous. Nature. 1970 Aug 1;227(5257):437–437. doi: 10.1038/227437a0. [DOI] [PubMed] [Google Scholar]
  2. AXELRAD A. A., STEEVES R. A. ASSAY FOR FRIEND LEUKEMIA VIRUS: RAPID QUANTITATIVE METHOD BASED ON ENUMERATION OF MACROSCOPIC SPLEEN FOCI IN MICE. Virology. 1964 Nov;24:513–518. doi: 10.1016/0042-6822(64)90199-0. [DOI] [PubMed] [Google Scholar]
  3. Berkower A. S., Lilly F., Soeiro R. Expression of viral RNA in Friend virus-induced erythroleukemia cells. Cell. 1980 Mar;19(3):637–642. doi: 10.1016/s0092-8674(80)80040-7. [DOI] [PubMed] [Google Scholar]
  4. Bernstein A., Mak T. W., Stephenson J. R. The Friend virus genome: evidence for the stable association of MuLV sequences and sequences involved in erythroleukemic transformation. Cell. 1977 Sep;12(1):287–294. doi: 10.1016/0092-8674(77)90206-9. [DOI] [PubMed] [Google Scholar]
  5. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  6. Dresler S., Ruta M., Murray M. J., Kabat D. Glycoprotein encoded by the Friend spleen focus-forming virus. J Virol. 1979 May;30(2):564–575. doi: 10.1128/jvi.30.2.564-575.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eckner R. J., Hettrick K. L. Persistence and pathogenicity of defective Friend spleen focus-forming virus. Decreased transplantability of hemopoietic cells as a marker for preleukemic change. J Exp Med. 1979 Feb 1;149(2):340–357. doi: 10.1084/jem.149.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edwards S. A., Fan H. gag-Related polyproteins of Moloney murine leukemia virus: evidence for independent synthesis of glycosylated and unglycosylated forms. J Virol. 1979 May;30(2):551–563. doi: 10.1128/jvi.30.2.551-563.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Hartley J. W., Rowe W. P. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4676–4680. doi: 10.1073/pnas.74.10.4676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Evans L. H., Dresler S., Kabat D. Synthesis and glycosylation of polyprotein precursors to the internal core proteins of Friend murine leukemia virus. J Virol. 1977 Dec;24(3):865–874. doi: 10.1128/jvi.24.3.865-874.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Famulari N. G., Buchhagen D. L., Klenk H. D., Fleissner E. Presence of murine leukemia virus envelope proteins gp70 and p15(E) in a common polyprotein of infected cells. J Virol. 1976 Nov;20(2):501–508. doi: 10.1128/jvi.20.2.501-508.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fischinger P. J., Dunlop N. M., Blevins C. S. Identification of virus found in mouse lymphomas induced by HIX murine oncornavirus. J Virol. 1978 May;26(2):532–535. doi: 10.1128/jvi.26.2.532-535.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Freedman H. A., Lilly F. Properties of cell lines derived from tumors induced by Friend virus in BALB/c and BALB/c-H-2b mice. J Exp Med. 1975 Jul 1;142(1):212–223. doi: 10.1084/jem.142.1.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Freedman H. A., Lilly F., Steeves R. A. Antigenic properties of cultured tumor cell lines derived from spleens of Friend virus-infected BALB/c and BALB/c-H-2b mice. J Exp Med. 1975 Dec 1;142(6):1365–1376. doi: 10.1084/jem.142.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Freedman H. A., Lilly F., Strand M., August J. T. Variations in viral gene expression in Friend virus-transformed cell lines congenic with respect to the H-2 locus. Cell. 1978 Jan;13(1):33–40. doi: 10.1016/0092-8674(78)90135-6. [DOI] [PubMed] [Google Scholar]
  16. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  19. Kopchick J. J., Karshin W. L., Arlinghaus R. B. Tryptic peptide analysis of gag and gag-pol gene products of Rauscher murine leukemia virus. J Virol. 1979 May;30(2):610–623. doi: 10.1128/jvi.30.2.610-623.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Naso R. B., Arcement L. J., Karshin W. L., Jamjoom G. A., Arlinghaus R. B. A fucose-deficient glycoprotein precursor to Rauscher leukemia virus gp69/71. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2326–2330. doi: 10.1073/pnas.73.7.2326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Naso R. B., Karshin W. L., Wu Y. H., Arlinghaus R. B. Characterization of 40,000- and 25,000-dalton intermediate precursors to Rauscher murine leukemia virus gag gene products. J Virol. 1979 Oct;32(1):187–198. doi: 10.1128/jvi.32.1.187-198.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Plata F., Goodenow M. M., Lilly F. Studies of cloned Friend erythroleukemia tumor cells. Modulation of the tumor-specific cytologic T lymphocyte response by infectious Friend virus production in vitro. J Exp Med. 1980 Mar 1;151(3):726–742. doi: 10.1084/jem.151.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Racevskis J., Koch G. Synthesis and processing of viral proteins in Friend erythroleukemia cell lines. Virology. 1978 Jun 15;87(2):354–365. doi: 10.1016/0042-6822(78)90140-x. [DOI] [PubMed] [Google Scholar]
  24. Racevskis J., Koch G. Viral protein synthesis in Friend erythroleukemia cell lines. J Virol. 1977 Jan;21(1):328–337. doi: 10.1128/jvi.21.1.328-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  26. Ruscetti S. K., Linemeyer D., Feild J., Troxler D., Scolnick E. M. Characterization of a protein found in cells infected with the spleen focus-forming virus that shares immunological cross-reactivity with the gp70 found in mink cell focus-inducing virus particles. J Virol. 1979 Jun;30(3):787–798. doi: 10.1128/jvi.30.3.787-798.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ruscetti S., Troxler D., Linemeyer D., Scolnick E. Three laboratory strains of spleen focus-forming virus: comparison of their genomes and translational products. J Virol. 1980 Jan;33(1):140–151. doi: 10.1128/jvi.33.1.140-151.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schultz A. M., Rabin E. H., Oroszlan S. Post-translational modification of Rauscher leukemia virus precursor polyproteins encoded by the gag gene. J Virol. 1979 Apr;30(1):255–266. doi: 10.1128/jvi.30.1.255-266.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Steeves R. A. Editorial: Spleen focus-forming virus in Friend and Rauscher leukemia virus preparations. J Natl Cancer Inst. 1975 Feb;54(2):289–297. doi: 10.1093/jnci/54.2.289. [DOI] [PubMed] [Google Scholar]
  30. Troxler D. H., Lowy D., Howk R., Young H., Scolnick E. M. Friend strain of spleen focus-forming virus is a recombinant between ecotropic murine type C virus and the env gene region of xenotropic type C virus. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4671–4675. doi: 10.1073/pnas.74.10.4671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Troxler D. H., Parks W. P., Vass W. C., Scolnick E. M. Isolation of a fibroblast nonproducer cell line containing the Friend strain of the spleen focus-forming virus. Virology. 1977 Feb;76(2):602–615. doi: 10.1016/0042-6822(77)90242-2. [DOI] [PubMed] [Google Scholar]
  32. Yoshida M., Yoshikura H. Analysis of spleen focus-forming virus-specific RNA sequences coding for spleen focus-forming virus-specific glycoprotein with a molecular weight of 55,000 (gp55). J Virol. 1980 Feb;33(2):587–596. doi: 10.1128/jvi.33.2.587-596.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. van Griensven L. J., Vogt M. Rauscher "mink cell focus-inducing" (MCF) virus causes erythroleukemia in mice: its isolation and properties. Virology. 1980 Mar;101(2):376–388. doi: 10.1016/0042-6822(80)90451-1. [DOI] [PubMed] [Google Scholar]

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