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. 1981 Mar;37(3):899–907. doi: 10.1128/jvi.37.3.899-907.1981

Avian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping.

S S Hu, M M Lai, T C Wong, R S Cohen, M Sevoian
PMCID: PMC171086  PMID: 6262534

Abstract

The genome structure of defective, oncogenic avian reticuloendotheliosis virus (REV) was studied by heteroduplex mapping between the full-length complementary DNA of the helper virus REV-T1 and the 30S REV RNA. The REV genome (5.5 kilobases) had a deletion of 3.69 kilobases in the gag-pol region, confirming the genetic defectiveness of REV. In addition, REV lacked the sequences corresponding to the env gene but contained, instead, a contiguous stretch (1.6 to 1.9 kilobases) of the specific sequences presumably related to viral oncogenicity. Unlike those of other avian acute leukemia viruses, the transformation-specific sequences of REV were not contiguous with the gag-pol deletion. Thus, REV has a genome structure similar to that of a defective mink cell focus-inducing virus or a defective murine sarcoma virus. An additional class of heteroduplex molecules containing the gag-pol deletion and two other smaller deletion loops was observed. These molecules probably represented recombinants between the oncogenic REV and its helper virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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