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. 1981 Mar;37(3):976–986. doi: 10.1128/jvi.37.3.976-986.1981

New genes in the left arm of the bacteriophage phi 80 chromosome.

P Youderian, J King
PMCID: PMC171095  PMID: 7230327

Abstract

We describe the isolation and partial genetic characterization of 247 amber (suppressor-sensitive) mutants of temperate bacteriophage phi 80 of Escherichia coli. Of these 247 mutants, the mutations of 201 mapped to the left arm of the phi 809 chromosome and the mutations of 39 mapped to the right arm of the genome. Complementation tests among these and previously described left arm mutants defined five additional genes in the left arm of the chromosome. The positions of these genes are consistent with the hypothesis that four of them represent functions essential for phi 80 tail assembly and one represents a capsid assembly function, probably the major coat protein. The identification of these genes brings the phi 80 genome into closer correspondence with the organization of the phage lambda genome. Two- and three-factor crosses performed between mutants with defects in each of the previously identified genes and mutants with defects in the five new genes allowed us to construct a consistent, roughly additive recombination map of the left arm of the bacteriophage phi 80 genome.

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Selected References

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  1. Blattner F. R., Williams B. G., Blechl A. E., Denniston-Thompson K., Faber H. E., Furlong L., Grunwald D. J., Kiefer D. O., Moore D. D., Schumm J. W. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977 Apr 8;196(4286):161–169. doi: 10.1126/science.847462. [DOI] [PubMed] [Google Scholar]
  2. Goldberg A. R., Howe M. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis. Virology. 1969 May;38(1):200–202. doi: 10.1016/0042-6822(69)90148-2. [DOI] [PubMed] [Google Scholar]
  3. Katsura I., Kühl P. W. Morphogenesis of the tail of bacteriophage lambda. III. Morphogenetic pathway. J Mol Biol. 1975 Jan 25;91(3):257–273. doi: 10.1016/0022-2836(75)90379-4. [DOI] [PubMed] [Google Scholar]
  4. Katsura I. Morphogenesis of bacteriophage lambda tail. Polymorphism in the assembly of the major tail protein. J Mol Biol. 1976 Nov 5;107(3):307–326. doi: 10.1016/s0022-2836(76)80007-1. [DOI] [PubMed] [Google Scholar]
  5. Kikuchi Y., King J. Genetic control of bacteriophage T4 baseplate morphogenesis. III. Formation of the central plug and overall assembly pathway. J Mol Biol. 1975 Dec 25;99(4):695–716. doi: 10.1016/s0022-2836(75)80180-x. [DOI] [PubMed] [Google Scholar]
  6. Lozeron H. A., Szybalski W. Congruent transcriptional controls and heterology of base sequences in coliphages lambda and phi-80. Virology. 1969 Nov;39(3):373–388. doi: 10.1016/0042-6822(69)90085-3. [DOI] [PubMed] [Google Scholar]
  7. Murialdo H., Becker A. Head morphogenesis of complex double-stranded deoxyribonucleic acid bacteriophages. Microbiol Rev. 1978 Sep;42(3):529–576. doi: 10.1128/mr.42.3.529-576.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parkinson J. S. Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics. 1968 Jul;59(3):311–325. doi: 10.1093/genetics/59.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rybchin V. N., Furaev V. V., Grigor'ev P. S., Ziuzenkova E. P., Kochnev I. G. Kartirovanie novykh strukturnykh genov faga phi80. Genetika. 1978 Sep;14(9):1539–1542. [PubMed] [Google Scholar]
  10. SIGNER E. R. RECOMBINATION BETWEEN COLIPHAGES LAMBDA AND PHI-80. Virology. 1964 Apr;22:650–651. doi: 10.1016/0042-6822(64)90090-x. [DOI] [PubMed] [Google Scholar]
  11. STAHL F. W., EDGAR R. S., STEINBERG J. THE LINKAGE MAP OF BACTERIOPHAGE T4. Genetics. 1964 Oct;50:539–552. doi: 10.1093/genetics/50.4.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sato K. Genetic map of bacteriophage phi80: genes on the right arm. Virology. 1970 Apr;40(4):1067–1069. doi: 10.1016/0042-6822(70)90156-x. [DOI] [PubMed] [Google Scholar]
  13. Sato K., Nishimune Y., Sato M., Numich R., Matsushiro A. Suppressor-sensitive mutants of coliphage phi-80. Virology. 1968 Apr;34(4):637–649. [PubMed] [Google Scholar]
  14. Weigle J. J. Induction of Mutations in a Bacterial Virus. Proc Natl Acad Sci U S A. 1953 Jul;39(7):628–636. doi: 10.1073/pnas.39.7.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weigle J. Assembly of phage lambda in vitro. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1462–1466. doi: 10.1073/pnas.55.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamagishi H., Nakamura K., Ozeki H. Cohesion occurring between DNA molecules of temperate phages phi 80 and lambda or phi 81. Biochem Biophys Res Commun. 1965 Sep 22;20(6):727–732. doi: 10.1016/0006-291x(65)90077-x. [DOI] [PubMed] [Google Scholar]

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