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. 1981 Mar;37(3):1075–1078. doi: 10.1128/jvi.37.3.1075-1078.1981

Early RNAs in SP82- and SP01-infected Bacillus subtilis may be processed.

J S Downard, H R Whiteley
PMCID: PMC171106  PMID: 6785448

Abstract

Transcription of SP82 and SP01 DNAs in vitro by Bacillus subtilis RNA polymerase yielded mostly large RNA species, with many in excess of 1,500 bases in length, whereas most of the RNAs synthesized in vivo early in infection were much smaller. Addition of an extract from uninfected B. subtilis to reaction mixtures containing RNAs synthesized in vitro generated additional discrete RNAs whose mobilities on polyacrylamide gels matched the mobilities of some of the smaller RNAs synthesized in vivo.

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Selected References

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  1. Abelson J. RNA processing and the intervening sequence problem. Annu Rev Biochem. 1979;48:1035–1069. doi: 10.1146/annurev.bi.48.070179.005131. [DOI] [PubMed] [Google Scholar]
  2. Achberger E. C., Whiteley H. R. The interaction of Escherichia coli core RNA polymerase with specificity-determining subunits derived from unmodified and SP82-modified Bacillus subtilis RNA polymerase. J Biol Chem. 1980 Dec 25;255(24):11957–11964. [PubMed] [Google Scholar]
  3. Fujita D. J., Ohlsson-Wilhelm B. M., Geiduschek E. P. Transcription during bacteriophage SPO1 development: mutations affecting the program of viral transcription. J Mol Biol. 1971 Apr 28;57(2):301–317. doi: 10.1016/0022-2836(71)90348-2. [DOI] [PubMed] [Google Scholar]
  4. Gage L. P., Geiduschek E. P. RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA. J Mol Biol. 1971 Apr 28;57(2):279–297. doi: 10.1016/0022-2836(71)90346-9. [DOI] [PubMed] [Google Scholar]
  5. Gegenheimer P., Watson N., Apirion D. Multiple pathways for primary processing of ribosomal RNA in Escherichia coli. J Biol Chem. 1977 May 10;252(9):3064–3073. [PubMed] [Google Scholar]
  6. Hagen F., Young E. T. Regulation of synthesis of bacteriophage T7 lysozyme mRNA. Virology. 1973 Sep;55(1):231–241. doi: 10.1016/s0042-6822(73)81026-8. [DOI] [PubMed] [Google Scholar]
  7. Kawamura F., Ito J. Transcription of the genome of bacteriophage phi 29: isolation and mapping of the major early mRNA synthesized in vivo and in vitro. J Virol. 1977 Sep;23(3):562–577. doi: 10.1128/jvi.23.3.562-577.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lawrie J. M., Downard J. S., Whiteley H. R. Bacillus subtilis bacteriophages SP82, SPO1, and phie: a comparison of DNAs and of peptides synthesized during infection. J Virol. 1978 Sep;27(3):725–737. doi: 10.1128/jvi.27.3.725-737.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lawrie J. M., Spiegelman G. B., Whiteley H. R. DNA strand specificity of temporal RNA classes produced during infection of Bacillus subtilis by SP82. J Virol. 1976 Aug;19(2):359–373. doi: 10.1128/jvi.19.2.359-373.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Panganiban A. T., Whiteley H. R. Analysis of bacteriophage SP82 major "early" in vitro transcripts. J Virol. 1981 Jan;37(1):372–382. doi: 10.1128/jvi.37.1.372-382.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reeve J. N., Mertens G., Amann E. Early development of bacteriophages SP01 and SP82G in minicells of Bacillus subtilis. J Mol Biol. 1978 Apr 5;120(2):183–207. doi: 10.1016/0022-2836(78)90064-5. [DOI] [PubMed] [Google Scholar]
  12. Sogin M. L., Pace B., Pace N. R. Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis. J Biol Chem. 1977 Feb 25;252(4):1350–1357. [PubMed] [Google Scholar]
  13. Sogin M. L., Pace N. R. Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis. J Biol Chem. 1976 Jun 10;251(11):3480–3488. [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Spiegelman G. B., Whiteley H. R. Bacteriophage SP82 induced modifications of Bacillus subtilis RNA polymerase result in the recognition of additional RNA synthesis initiation sites on phage DNA. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1058–1065. doi: 10.1016/0006-291x(78)91458-4. [DOI] [PubMed] [Google Scholar]
  16. Spiegelman G. B., Whiteley H. R. In vivo and in vitro transcription by ribonucleic acid polymerase from SP82-infected Bacillus subtilis. J Biol Chem. 1974 Mar 10;249(5):1483–1489. [PubMed] [Google Scholar]
  17. Talkington C., Pero J. Restriction fragment analysis of the temporal program of bacteriophage SPO1 transcription and its control by phage-modified RNA polymerases. Virology. 1977 Dec;83(2):365–379. doi: 10.1016/0042-6822(77)90181-7. [DOI] [PubMed] [Google Scholar]

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