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. 1981 Apr;38(1):50–58. doi: 10.1128/jvi.38.1.50-58.1981

Cloning of herpes simplex virus type 1 sequences representing the whole genome.

A L Goldin, R M Sandri-Goldin, M Levine, J C Glorioso
PMCID: PMC171125  PMID: 6264114

Abstract

Sequences representative of the whole genome of herpes simplex virus type 1 (HSV-1) strain KOS were cloned in the plasmid vector pBR325 in the form of EcoRI-generated DNA fragments. The cloned fragments were identified by digestion of the chimeric plasmid DNA with restriction enzymes EcoRI or EcoRI and BglII followed by comparison of their electrophoretic mobilities in agarose gels with that of similarly digested HSV-1 virion DNA. The cloned fragments showed the same migration patterns as the corresponding fragments from restricted virion DNA, indicating that no major insertions or deletions were present. The presence of HSV-1 sequences in the chimeric plasmids was confirmed by hybridization of plasmid DNA to HSV-1 virion DNA. Additionally, some of the cloned fragments were shown to be biologicaly active in that they efficiently rescued three HSV-1 temperature-sensitive mutants in cotransfection marker rescue experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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