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. 1981 Apr;38(1):398–402. doi: 10.1128/jvi.38.1.398-402.1981

Direct Proof of the 5′ to 3′ Transcriptional Jump During Reverse Transcription of the Avian Retrovirus Genome by DNA Sequencing

Charles A Omer 1, J Thomas Parsons 2, Anthony J Faras 1
PMCID: PMC171166  PMID: 6165832

Abstract

DNA sequence analysis of avian sarcoma virus in vitro-synthesized complementary DNA and in vivo-synthesized, molecularly cloned viral DNA provides unequivocal proof that only one copy of the terminally redundant viral RNA sequence is copied during reverse transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clayman C. H., Mosharrafa E. T., Anderson D. L., Feras A. J. Circular forms of DNA synthesized by Rous sarcoma virus in vitro. Science. 1979 Nov 2;206(4418):582–584. doi: 10.1126/science.91199. [DOI] [PubMed] [Google Scholar]
  2. Clayman C. H., Mosharrafa E., Faras A. J. In vitro synthesis of infectious transforming DNA by the avian sarcoma virus reverse transcriptase. J Virol. 1979 Jan;29(1):242–249. doi: 10.1128/jvi.29.1.242-249.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coffin J. M., Haseltine W. A. Nucleotide sequence of Rous sarcoma virus RNA at the initiation site of DNA synthesis: The 102nd nucleotide is U. J Mol Biol. 1977 Dec 15;117(3):805–814. doi: 10.1016/0022-2836(77)90071-7. [DOI] [PubMed] [Google Scholar]
  4. Coffin J. M., Haseltine W. A. Terminal redundancy and the origin of replication of Rous sarcoma virus RNA. Proc Natl Acad Sci U S A. 1977 May;74(5):1908–1912. doi: 10.1073/pnas.74.5.1908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collett M. S., Dierks P., Parsons J. T., Faras A. J. RNase H hydrolysis of the 5' terminus of the avian sarcoma virus genome during reverse transcription. Nature. 1978 Mar 9;272(5649):181–184. doi: 10.1038/272181a0. [DOI] [PubMed] [Google Scholar]
  6. Collett M. S., Faras A. J. Avian retrovirus RNA-directed DNA synthesis: transcription at the 5' terminus of the viral genome and the functional role for the viral terminal redundancy. Virology. 1978 May 15;86(2):297–311. doi: 10.1016/0042-6822(78)90072-7. [DOI] [PubMed] [Google Scholar]
  7. Cordell B., Stavnezer E., Friedrich R., Bishop J. M., Goodman H. M. Nucleotide sequence that binds primer for DNA synthesis to the avian sarcoma virus genome. J Virol. 1976 Aug;19(2):548–558. doi: 10.1128/jvi.19.2.548-558.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahlberg J. E., Harada F., Sawyer R. C. Structure and properties of an RNA primer for initiation of Rous sarcoma virus DNA synthesis in vitro. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):925–932. doi: 10.1101/sqb.1974.039.01.108. [DOI] [PubMed] [Google Scholar]
  9. Dhar R., McClements W. L., Enquist L. W., Vande Woude G. F. Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937–3941. doi: 10.1073/pnas.77.7.3937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilboa E., Goff S., Shields A., Yoshimura F., Mitra S., Baltimore D. In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus. Cell. 1979 Apr;16(4):863–874. doi: 10.1016/0092-8674(79)90101-6. [DOI] [PubMed] [Google Scholar]
  11. Gilboa E., Mitra S. W., Goff S., Baltimore D. A detailed model of reverse transcription and tests of crucial aspects. Cell. 1979 Sep;18(1):93–100. doi: 10.1016/0092-8674(79)90357-x. [DOI] [PubMed] [Google Scholar]
  12. Haseltine W. A., Coffin J. M., Hageman T. C. Structure of products of the Moloney murine leukemia virus endogenous DNA polymerase reaction. J Virol. 1979 Apr;30(1):375–383. doi: 10.1128/jvi.30.1.375-383.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Haseltine W. A., Maxam A. M., Gilbert W. Rous sarcoma virus genome is terminally redundant: the 5' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):989–993. doi: 10.1073/pnas.74.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Highfield P. E., Rafield L. F., Gilmer T. M., Parsons J. T. Molecular cloning of avian sarcoma virus closed circular DNA: structural and biological characterization of three recombinant clones. J Virol. 1980 Oct;36(1):271–279. doi: 10.1128/jvi.36.1.271-279.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mitra S. W., Goff S., Gilboa E., Baltimore D. Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4355–4359. doi: 10.1073/pnas.76.9.4355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Novak U., Friedrich R., Moelling K. Elongation of DNA complementary to the 5' end of the avian sarcoma virus genome by the virion-associated RNA-dependent DNA polymerase. J Virol. 1979 May;30(2):438–452. doi: 10.1128/jvi.30.2.438-452.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schwartz D. E., Zamecnik P. C., Weith H. L. Rous sarcoma virus genome is terminally redundant: the 3' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):994–998. doi: 10.1073/pnas.74.3.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shimotohno K., Mizutani S., Temin H. M. Sequence of retrovirus provirus resembles that of bacterial transposable elements. Nature. 1980 Jun 19;285(5766):550–554. doi: 10.1038/285550a0. [DOI] [PubMed] [Google Scholar]
  21. Shine J., Czernilofsky A. P., Friedrich R., Bishop J. M., Goodman H. M. Nucleotide sequence at the 5' terminus of the avian sarcoma virus genome. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1473–1477. doi: 10.1073/pnas.74.4.1473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shoemaker C., Goff S., Gilboa E., Paskind M., Mitra S. W., Baltimore D. Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3932–3936. doi: 10.1073/pnas.77.7.3932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stoll E., Billeter M. A., Palmenberg A., Weissmann C. Avian myeloblastosis virus RNA is terminally redundant: implications for the mechanism of retrovirus replication. Cell. 1977 Sep;12(1):57–72. doi: 10.1016/0092-8674(77)90185-4. [DOI] [PubMed] [Google Scholar]
  24. Sutcliffe J. G., Shinnick T. M., Verma I. M., Lerner R. A. Nucleotide sequence of Moloney leukemia virus: 3' end reveals details of replications, analogy to bacterial transposons, and an unexpected gene. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3302–3306. doi: 10.1073/pnas.77.6.3302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van Beveren C., Goddard J. G., Berns A., Verma I. M. Structure of Moloney murine leukemia viral DNA: nucleotide sequence of the 5' long terminal repeat and adjacent cellular sequences. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3307–3311. doi: 10.1073/pnas.77.6.3307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Walseth T. F., Johnson R. A. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP. Biochim Biophys Acta. 1979 Mar 28;562(1):11–31. doi: 10.1016/0005-2787(79)90122-9. [DOI] [PubMed] [Google Scholar]

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