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Journal of Virology logoLink to Journal of Virology
. 1981 Jun;38(3):952–957. doi: 10.1128/jvi.38.3.952-957.1981

Analysis of the myeloproliferative sarcoma virus genome: limited changes in the prototype lead to altered target cell specificity.

I B Pragnell, A Fusco, C Arbuthnott, F Smadja-Joffe, B Klein, C Jasmin, W Ostertag
PMCID: PMC171233  PMID: 6264165

Abstract

The myeloproliferative sarcoma virus (MPSV) derived from Moloney sarcoma virus (MSV-Mol) is a unique sarcoma virus which causes expansion of the hematopoietic stem cell compartment as well as the erythroid and myeloid cell lineages. MPSV also induces spleen focus formation in adult mice as do Friend and Rauscher viruses. Analysis of the MPSV genome on methyl mercury gels showed that the genome size is 7.0 kilobases, which is larger than the defective genome of any known MSV-Mol isolate. Hybridization analysis with specific cDNA probes showed that MPSV is a modified sarcoma virus with no sequences in the unique region of the defective sarcoma genome related to unique Friend virus sequences. The only viral sequences in the defective genome other than helper virus-related sequences are derived from the Moloney sarcoma virus genome with no new cellular sequences added. There was no evidence for induction of xenotropic virus sequences in MPSV-infected spleens of DBA/2J mice, indicating that spleen focus formation can be obtained by different mechanisms.

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Selected References

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  1. Bernstein A., Gamble C., Penrose D., Mak T. W. Presence and expression of Friend erythroleukemia virus-related sequences in normal and leukemic mouse tissues. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4455–4459. doi: 10.1073/pnas.76.9.4455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bilello J. A., Colletta G., Warnecke G., Koch G., Frisby D., Pragnell I. B., Ostertag W. Analysis of the expression of spleen focus-forming virus (SFFV)-related RNA and gp55, a Friend and Rauscher virus-specific protein. Virology. 1980 Dec;107(2):331–344. doi: 10.1016/0042-6822(80)90301-3. [DOI] [PubMed] [Google Scholar]
  3. Chirigos M. A., Scott D., Turner W., Perk K. Biological, pathological and physical characterization of a possible variant of a murine sarcoma virus (Moloney). Int J Cancer. 1968 Mar 15;3(2):223–227. doi: 10.1002/ijc.2910030207. [DOI] [PubMed] [Google Scholar]
  4. Dube S., Kung H. J., Bender W., Davidson N., Ostertag W. Size, subunit composition, and secondary structure of the Friend virus genome. J Virol. 1976 Oct;20(1):264–272. doi: 10.1128/jvi.20.1.264-272.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duesberg P. H. Transforming genes of retroviruses. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):13–29. doi: 10.1101/sqb.1980.044.01.005. [DOI] [PubMed] [Google Scholar]
  6. Frankel A. E., Fischinger P. J. Nucleotide sequences in mouse DNA and RNA specific for Moloney sarcoma virus. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3705–3709. doi: 10.1073/pnas.73.10.3705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Graf T., Beug H. Avian leukemia viruses: interaction with their target cells in vivo and in vitro. Biochim Biophys Acta. 1978 Nov 17;516(3):269–299. doi: 10.1016/0304-419x(78)90011-2. [DOI] [PubMed] [Google Scholar]
  8. Hankins W. D., Kost T. A., Koury M. J., Krantz S. B. Erythroid bursts produced by Friend leukaemia virus in vitro. Nature. 1978 Nov 30;276(5687):506–508. doi: 10.1038/276506a0. [DOI] [PubMed] [Google Scholar]
  9. Le Bousse-Kerdiles M. C., Smadja-Joffe F., Klein B., Caillou B., Jasmin C. Study of a virus-induced myeloproliferative syndrome associated with tumor formation in mice. Eur J Cancer. 1980 Jan;16(1):43–51. doi: 10.1016/0014-2964(80)90106-1. [DOI] [PubMed] [Google Scholar]
  10. Maisel J., Dina D., Duesberg P. Murine sarcoma viruses: the helper-independence reported for a Moloney variant is unconfirmed; distinct strains differ in the size of their RNAs. Virology. 1977 Jan;76(1):295–312. doi: 10.1016/0042-6822(77)90304-x. [DOI] [PubMed] [Google Scholar]
  11. Ostertag W., Odaka T., Smadja-Joffe F., Jasmin C. Inheritance of susceptibility to the myeloproliferative sarcoma virus: effect of the Fv-2 locus and evidence for a myeloproliferative sarcoma virus resistance locus. J Virol. 1981 Feb;37(2):541–548. doi: 10.1128/jvi.37.2.541-548.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ostertag W., Pragnell I. B. Changes in genome composition of the Friend virus complex in erythroleukemia cells during the course of differentiation induced by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3278–3282. doi: 10.1073/pnas.75.7.3278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ostertag W., Vehmeyer K., Fagg B., Pragnell I. B., Paetz W., Le Bousse M. C., Smadja-Joffe F., Klein B., Jasmin C., Eisen H. Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice. J Virol. 1980 Feb;33(2):573–582. doi: 10.1128/jvi.33.2.573-582.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pragnell I. B., McNab A., Harrison P. R., Osterag W. Are spleen focus-forming virus sequences related to xenotropic viruses and expressed specifically in normal erythroid cells? Nature. 1978 Mar 30;272(5652):456–458. doi: 10.1038/272456a0. [DOI] [PubMed] [Google Scholar]
  15. Pragnell I. B., Ostertag W., Paul J. The expression of viral and globin genes during differentiation of the Friend cell. Exp Cell Res. 1977 Sep;108(2):269–278. doi: 10.1016/s0014-4827(77)80034-7. [DOI] [PubMed] [Google Scholar]
  16. Roussel M., Saule S., Lagrou C., Rommens C., Beug H., Graf T., Stehelin D. Three new types of viral oncogene of cellular origin specific for haematopoietic cell transformation. Nature. 1979 Oct 11;281(5731):452–455. doi: 10.1038/281452a0. [DOI] [PubMed] [Google Scholar]
  17. Scher C. D., Siegler R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature. 1975 Feb 27;253(5494):729–731. doi: 10.1038/253729a0. [DOI] [PubMed] [Google Scholar]
  18. Scolnick E. M., Howk R. S., Anisowicz A., Peebles P. T., Scher C. D., Parks W. P. Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4650–4654. doi: 10.1073/pnas.72.11.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scolnick E. M., Rands E., Williams D., Parks W. P. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus. J Virol. 1973 Sep;12(3):458–463. doi: 10.1128/jvi.12.3.458-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sheiness D., Bishop J. M. DNA and RNA from uninfected vertebrate cells contain nucleotide sequences related to the putative transforming gene of avian myelocytomatosis virus. J Virol. 1979 Aug;31(2):514–521. doi: 10.1128/jvi.31.2.514-521.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shields A., Goff S., Paskind M., Otto G., Baltimore D. Structure of the Abelson murine leukemia virus genome. Cell. 1979 Dec;18(4):955–962. doi: 10.1016/0092-8674(79)90208-3. [DOI] [PubMed] [Google Scholar]
  22. Sklar M. D., White B. J., Rowe W. P. Initiation of oncogenic transformation of mouse lymphocytes in vitro by Abelson leukemia virus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4077–4081. doi: 10.1073/pnas.71.10.4077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stehelin D., Varmus H. E., Bishop J. M., Vogt P. K. DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA. Nature. 1976 Mar 11;260(5547):170–173. doi: 10.1038/260170a0. [DOI] [PubMed] [Google Scholar]
  24. Troxler D. H., Boyars J. K., Parks W. P., Scolnick E. M. Friend strain of spleen focus-forming virus: a recombinant between mouse type C ecotropic viral sequences and sequences related to xenotropic virus. J Virol. 1977 May;22(2):361–372. doi: 10.1128/jvi.22.2.361-372.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Troxler D. H., Lowy D., Howk R., Young H., Scolnick E. M. Friend strain of spleen focus-forming virus is a recombinant between ecotropic murine type C virus and the env gene region of xenotropic type C virus. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4671–4675. doi: 10.1073/pnas.74.10.4671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ullrich A., Shine J., Chirgwin J., Pictet R., Tischer E., Rutter W. J., Goodman H. M. Rat insulin genes: construction of plasmids containing the coding sequences. Science. 1977 Jun 17;196(4296):1313–1319. doi: 10.1126/science.325648. [DOI] [PubMed] [Google Scholar]
  27. Wei C. M., Lowy D. R., Scolnick E. M. Mapping of transforming region of the Harvey murine sarcoma virus genome by using insertion-deletion mutants constructed in vitro. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4674–4678. doi: 10.1073/pnas.77.8.4674. [DOI] [PMC free article] [PubMed] [Google Scholar]

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