Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1970 Mar;6(3):407–411.

Studies of the third component of complement in synovial fluid from arthritic patients. I. Immunochemical quantitation and relation to total complement

B Lundh, H Hedberg, Anna-Brita Laurell
PMCID: PMC1712699  PMID: 5442523

Abstract

In a study of forty-one patients, most of whom had longstanding rheumatoid arthritis, a comparison was made in serum and synovial fluid between total complement activity (CH50) and C3, measured immunochemically. Nineteen out of twenty-seven patients with rheumatoid arthritis and four out of four cases of systemic lupus erythematosus (SLE) or SLE-like syndromes had a depressed CH50 value in the synovial fluid, while ten cases with a non-rheumatoid form of arthritis all had normal activity.

The total C activity in serum was normal or increased in most cases. While no correlation was found between CH50 and C3 in serum, there was a statistically highly significant correlation in synovial fluid, indicating that C3 determinations can be used to reveal alterations in C activity of synovial fluid.

Full text

PDF
407

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANSELL B. M., BYWATERS E. G. Diagnosis of "probable" Still's disease and its outcome. Ann Rheum Dis. 1962 Sep;21:253–262. doi: 10.1136/ard.21.3.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FOSTIROPOULOS G., AUSTEN K. F., BLOCH K. J. TOTAL HEMOLYTIC COMPLEMENT (CH50) AND SECOND COMPONENT OF COMPLEMENT (C2 HU) ACTIVITY IN SERUM AND SYNOVIAL FLUID. Arthritis Rheum. 1965 Apr;8:219–232. doi: 10.1002/art.1780080206. [DOI] [PubMed] [Google Scholar]
  3. KLEMPERER M. R., GOTOFF S. P., ALPER C. A., LEVIN A. S., ROSEN F. S. ESTIMATION OF THE SERUM BETA-1C GLOBULIN CONCENTRATION: ITS RELATION TO THE SERUM HEMOLYTIC COMPLEMENT TITER. Pediatrics. 1965 May;35:765–769. [PubMed] [Google Scholar]
  4. Kohler P. F., Müller-Eberhard H. J. Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults. J Immunol. 1967 Dec;99(6):1211–1216. [PubMed] [Google Scholar]
  5. Laurell A. B. Effect of trypsin on complement components in normal human serum. Acta Pathol Microbiol Scand. 1968;72(1):139–146. doi: 10.1111/j.1699-0463.1968.tb00441.x. [DOI] [PubMed] [Google Scholar]
  6. Laurell C. B., Lundh B. Electrophoretic studies of the conversion products of serum beta-1C-globulin. Immunology. 1967 Mar;12(3):313–319. [PMC free article] [PubMed] [Google Scholar]
  7. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  8. OUDIN J. B. Specific precipitation in gels and its application to immunochemical analysis. Methods Med Res. 1952;5:335–378. [PubMed] [Google Scholar]
  9. Rodman W. S., Williams R. C., Jr, Bilka P. J., Müller-Eberhard H. J. Immunofluorescent localization of the third and the fourth component of complement in synovial tissue from patients with rheumatoid arthritis. J Lab Clin Med. 1967 Jan;69(1):141–150. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES