Abstract
Electrophoresis in agarose gel containing antibodies can be used for quantitation of C4 in serum and plasma.
The normal range of the C4 concentration in serum and plasma varied between 40 and 200% of the standard pools. Low C4 values were found in systemic lupus erythematosus, in acquired haemolytic anaemia and in hereditary angioneurotic oedema.
Differences in C4 values were found between normal sera and the corresponding EDTA plasma, when tested after dilution in calcium-free and EDTA buffers, respectively. After storage of the samples for 1 day at 20°C or 37°C the C4 values decreased, most markedly in plasma; no further decrease was found on prolongation of storage, and the difference between serum and plasma C4 values disappeared. The plasma C4 levels gradually fell on storage at 4°C, but a clear difference between serum and plasma was still demonstrable after 5 days.
The method described is simple and quick and can be used routinely in large scale investigations.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cooper N. R., Müller-Eberhard H. J. A comparison of methods for the molecular quantitation of the fourth component of human complement. Immunochemistry. 1968 Mar;5(2):155–169. doi: 10.1016/0019-2791(68)90100-6. [DOI] [PubMed] [Google Scholar]
- DONALDSON V. H., ROSEN F. S. ACTION OF COMPLEMENT IN HEREDITARY ANGIONEUROTIC EDEMA: THE ROLE OF C'1-ESTERASE. J Clin Invest. 1964 Nov;43:2204–2213. doi: 10.1172/JCI105094. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAINES A. L., LEPOW I. H. STUDIES ON HUMAN C'1-ESTERASE. I. PURIFICATION AND ENZYMATIC PROPERTIES. J Immunol. 1964 Mar;92:456–467. [PubMed] [Google Scholar]
- JONSEN J., KASS E., HARBOE M. Complement and complement components in acquired hemolytic anemia with high titer cold antibodies. Acta Med Scand. 1961 Dec;170:725–729. doi: 10.1111/j.0954-6820.1961.tb00291.x. [DOI] [PubMed] [Google Scholar]
- Kohler P. F., Müller-Eberhard H. J. Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults. J Immunol. 1967 Dec;99(6):1211–1216. [PubMed] [Google Scholar]
- LEPOW I. H., NAFF G. B., TODD E. W., PENSKY J., HINZ C. F. Chromatographic resolution of the first component of human complement into three activities. J Exp Med. 1963 Jun 1;117:983–1008. doi: 10.1084/jem.117.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
- MORSE J. H., MULLER-EBERHARD H. J., KUNKEL H. G. Anti-nuclear factors and serum complement in systemic lupus erythematosus. Bull N Y Acad Med. 1962 Oct;38:641–651. [PMC free article] [PubMed] [Google Scholar]
- MUELLER-EBERHARD H. J., BIRO C. E. ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT. J Exp Med. 1963 Sep 1;118:447–466. doi: 10.1084/jem.118.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUELLER-EBERHARD H. J., LEPOW I. H. C'1 ESTERASE EFFECT ON ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF THE FOURTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 May 1;121:819–833. doi: 10.1084/jem.121.5.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenfeld S. I., Ruddy S., Austen K. F. Structural polymorphism of the fourth component of human complement. J Clin Invest. 1969 Dec;48(12):2283–2292. doi: 10.1172/JCI106194. [DOI] [PMC free article] [PubMed] [Google Scholar]