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. 1970 Jan;6(1):125–136.

Macrophage specificity of antiserum against thymus lymphocytes

B P Maclaurin, Judith A Humm
PMCID: PMC1712747  PMID: 5416350

Abstract

Potent antisera against thymus lymphocytes have been shown by light and electron microscopy, to cause an increase in close cytoplasmic contacts between macrophages in monolayer tissue culture with subsequent aggregation (macrophage interlinkage reaction). This anti-macrophage activity remains present at high dilutions of antiserum, but can be markedly reduced by prior absorption with thymus lymphocytes. Evidence is presented which indicates that anti-lymphocyte antibody may also have anti-macrophage specificity and it is suggested that part of the immunosuppressive activity of anti-lymphocyte serum may be a consequence of this additional specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abaza H. M., Nolan B., Watt J. G., Woodruff M. F. Effect of antilymphocytic serum on the survival of renal homotransplants in dogs. Transplantation. 1966 Sep;4(5):618–632. doi: 10.1097/00007890-196609000-00008. [DOI] [PubMed] [Google Scholar]
  2. Boak J. L., Fox M., Wilson R. E. Activity of lymphoid tissues from antilymphocyte-serum-treated mice. Lancet. 1967 Apr 8;1(7493):750–752. doi: 10.1016/s0140-6736(67)91366-9. [DOI] [PubMed] [Google Scholar]
  3. Currey H. L., Ziff M. Suppression of experimentally induced polyarthritis in the rat by heterologous anti-lymphocyte serum. Lancet. 1966 Oct 22;2(7469):889–891. doi: 10.1016/s0140-6736(66)91984-2. [DOI] [PubMed] [Google Scholar]
  4. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  5. Denman A. M., Denman E. J., Holborow E. J. Suppression of Coombs-positive haemolytic anaemia in NZB mice by antilymphocyte globulin. Lancet. 1967 May 20;1(7499):1084–1086. doi: 10.1016/s0140-6736(67)92653-0. [DOI] [PubMed] [Google Scholar]
  6. Dumonde D. C. The role of the macrophage in delayed hypersensitivity. Br Med Bull. 1967 Jan;23(1):9–14. doi: 10.1093/oxfordjournals.bmb.a070524. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. F., Tursi A., Playfair J. H., Torrigiani G., Zamir R., Roitt I. M. Immunosuppressive potency and in-vitro activity of antilymphocyte globulin. Lancet. 1969 Jan 11;1(7585):68–72. doi: 10.1016/s0140-6736(69)91089-7. [DOI] [PubMed] [Google Scholar]
  8. HARRIS J. E., FORD C. E. CELLULAR TRAFFIC OF THE THYMUS: EXPERIMENTS WITH CHROMOSOME MARKERS. EVIDENCE THAT THE THYMUS PLAYS AN INSTRUCTIONAL PART. Nature. 1964 Feb 29;201:884–885. doi: 10.1038/201884a0. [DOI] [PubMed] [Google Scholar]
  9. HOLUB M. Potentialities of the small lymphocyte as revealed by homotransplantation and autotransplantation expriments in diffusion chambers. Ann N Y Acad Sci. 1962 Oct 24;99:477–486. doi: 10.1111/j.1749-6632.1962.tb45329.x. [DOI] [PubMed] [Google Scholar]
  10. Huntley R. T., Taylor P. D., Iwasaki Y., Marchioro T. L., Jeejeebhoy H., Porter K. A., Starzl T. E. Use of anti-lymphocyte serum to prolong dog homograft survival. Surg Forum. 1966;17:230–233. [PMC free article] [PubMed] [Google Scholar]
  11. Loewi G., Temple A., Nind A. P., Axelrad M. A study of the effects of anti-macrophage sera. Immunology. 1969 Jan;16(1):99–106. [PMC free article] [PubMed] [Google Scholar]
  12. Maclaurin B. P. Macrophage interlinkage reaction induced by phytohaemagglutinin and by tuberculin. Aust J Exp Biol Med Sci. 1969 Feb;47(1):105–112. doi: 10.1038/icb.1969.10. [DOI] [PubMed] [Google Scholar]
  13. Marshall W. H., Rigo S. J., Melman S. Lymphocyte transformation and mitosis in vitro initiated by homologous macrophages. An improved method for histocompatibility testing. Lancet. 1966 Apr 2;1(7440):730–732. doi: 10.1016/s0140-6736(66)90889-0. [DOI] [PubMed] [Google Scholar]
  14. Monaco A. P., Wood M. L., Gray J. G., Russell P. S. Studies on heterologous anti-lymphocyte serum in mice. II. Effect on the immune response. J Immunol. 1966 Feb;96(2):229–238. [PubMed] [Google Scholar]
  15. Roberts A. N. Cellular localization and quantitation of tritiated antigen in mouse lymph nodes during early primary immune response. Am J Pathol. 1966 Nov;49(5):889–909. [PMC free article] [PubMed] [Google Scholar]
  16. Stuart A. E. Adherence of opsonized lymphocytes to macrophage cultures. Experientia. 1967 May 15;23(5):347–348. doi: 10.1007/BF02144508. [DOI] [PubMed] [Google Scholar]
  17. Unanue E. R. Properties and some uses of anti-macrophage antibodies. Nature. 1968 Apr 6;218(5136):36–38. doi: 10.1038/218036a0. [DOI] [PubMed] [Google Scholar]
  18. VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
  19. Volkman A. The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med. 1966 Aug 1;124(2):241–254. doi: 10.1084/jem.124.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WAKSMAN B. H., ARBOUYS S., ARNASON B. G. The use of specific "lymphocyte" antisera to inhibit hypersensitive reactions of the "delayed" type. J Exp Med. 1961 Dec 1;114:997–1022. doi: 10.1084/jem.114.6.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WOODRUFF M. F., ANDERSON N. F. THE EFFECT OF LYMPHOCYTE DEPLETION BY THORACIC DUCT FISTULA AND ADMINISTRATION OF ANTILYMPHOCYTIC SERUM ON THE SURVIVAL OF SKIN HOMOGRAFTS IN RATS. Ann N Y Acad Sci. 1964 Nov 30;120:119–128. doi: 10.1111/j.1749-6632.1964.tb34710.x. [DOI] [PubMed] [Google Scholar]

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