Abstract
Fourteen sera containing antinuclear antibodies were studied to determine whether the immunoglobulin G subclass of these antibodies correlated with the relative complement fixing activity of the antibodies or the presence or absence of lupus nephritis. Sera studied included eight with high complement fixing activity, all from patients with active lupus nephritis and six with low complement fixing activity, three from patients with lupus without nephritis and three from patients with antinuclear antibodies induced by procainamide. Purified antinuclear antibodies reactive with desoxyribonucleoprotein and desoxyribonuclease were prepared by elution, and subclass typing was carried out by radial immunoassay with specific antisera.
In general, subclasses represented were similar in frequency to the occurrence of the subclasses in normal immunoglobulin G. There was no striking predominance or absence of any single subclass which might correlate with presence of nephritis, or differing complement fixing activity of sera. There was a trend to a predominance of subclasses high in complement fixing activity in antinuclear antibodies from lupus patients with active nephritis and subclasses low in complement fixing activity from those without nephritis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CASALS S. P., FRIOU G. J., MYERS L. L. SIGNIFICANCE OF ANTIBODY TO DNA IN SYSTEMIC LUPUS ERYTHEMATOSUS. Arthritis Rheum. 1964 Aug;7:379–390. doi: 10.1002/art.1780070404. [DOI] [PubMed] [Google Scholar]
- FAHEY J. L., MCLAUGHLIN C. PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS. J Immunol. 1963 Oct;91:484–497. [PubMed] [Google Scholar]
- FRIOU G. J., FINCH S. C., DETRE K. D. Interaction of nuclei and globulin from lupus erythematosis serum demonstrated with fluorescent antibody. J Immunol. 1958 Apr;80(4):324–329. [PubMed] [Google Scholar]
- HOLMAN H., DEICHER H. R. The reaction of the lupus erythematosus (L.E.) cell factor with deoxyribonucleoprotein of the cell nucleus. J Clin Invest. 1959 Nov;38:2059–2072. doi: 10.1172/JCI103984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spiegelberg H. L., Weigle W. O. The production of antisera to human gammaGlobulin subclasses in rabbits using immunological unresponsiveness. J Immunol. 1968 Aug;101(2):377–380. [PubMed] [Google Scholar]
- Tojo T., Friou G. J. Lupus nephritis: varying complement-fixing properties of immunoglobulin G antibodies to antigens of cell nuclei. Science. 1968 Aug 30;161(3844):904–906. doi: 10.1126/science.161.3844.904. [DOI] [PubMed] [Google Scholar]
- Yount W. J., Dorner M. M., Kunkel H. G., Kabat E. A. Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers. J Exp Med. 1968 Mar 1;127(3):633–646. doi: 10.1084/jem.127.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]