Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1970 Jan;6(1):75–86.

Studies on eosinophil leucocyte migration. I. Eosinophil and neutrophil accumulation following antigen–antibody reactions in guinea-pig skin

A B Kay
PMCID: PMC1712766  PMID: 5416353

Abstract

Eosinophil leucocytes migrated into the site of PCA reactions mediated by those fractions of 7S guinea-pig IgG containing IgG1; neutrophils were associated with IgG1 and IgG2. Maximal eosinophil infiltration is seen at 12 hr and was associated with degranulation.

Intradermal histamine was not eosinophilotactic in guinea-pigs.

Preformed antigen–antibody complexes of IgG1 and IgG2 both promoted eosinophil and neutrophil migration in guinea-pig skin but slightly more eosinophils were seen following injections of complexes containing IgG1. Local eosinophilia and PCA activity were mediated by a relatively heat-stable element since these effects were demonstrable even after prolonged heating of fractions containing IgG1. Eosinophils were seen following injections of Compound 48/80 and this was accompanied by low mast cell counts; however, there was also some associated tissue destruction.

Full text

PDF
75

Images in this article

77Fig. 2
on p.77

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer R. K., Lowell F. C. Histamine and eosinophilotaxis. J Allergy. 1968 Aug;42(2):109–110. doi: 10.1016/0021-8707(68)90140-8. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed] [Google Scholar]
  3. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brocklehurst W. E., Colquhoun D. Adsorption and diffusion of gamma-globulin during passive sensitization of chopped guinea-pig lung. J Physiol. 1965 Dec;181(4):760–784. doi: 10.1113/jphysiol.1965.sp007796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen S. G., Sapp T. M. Experimental eosinophilia. 8. Cellular responses to altered globulins within cutaneous tissue. J Allergy. 1965 Sep-Oct;36(5):415–422. doi: 10.1016/0021-8707(65)90135-8. [DOI] [PubMed] [Google Scholar]
  6. FEINBERG S. M., STERNBERGER L. A. Action of histamine liberator compound 48/80 in the guinea pig; with a review of previous reports of its action in other species. J Allergy. 1955 Mar;26(2):170–179. doi: 10.1016/0021-8707(55)90052-6. [DOI] [PubMed] [Google Scholar]
  7. Felarca A. B., Lowell F. C. Failure to elicit histamine eosinophilotaxis in the skin of atopic man. Description of an improved technique. J Allergy. 1968 Feb;41(2):82–87. doi: 10.1016/0021-8707(68)90016-6. [DOI] [PubMed] [Google Scholar]
  8. Forsgren A. Protein A from Staphylococcus aureus. V. Reaction with guinea pig gamma-globulins. J Immunol. 1968 May;100(5):921–926. [PubMed] [Google Scholar]
  9. Ishizaka K., Ishizaka T. Induction of erythema-wheal reactions by soluble antigen-gammaE antibody complexes in humans. J Immunol. 1968 Jul;101(1):68–78. [PubMed] [Google Scholar]
  10. Keller H. U., Sorkin E. Studies on chemotaxis. 13. Differences in the chemotactic responses of neutrophil and eosinophil polymorphonuclear leucocytes. Int Arch Allergy Appl Immunol. 1969;35(3):279–287. [PubMed] [Google Scholar]
  11. LITT M. EOSINOPHILS AND ANTIGEN-ANTIBODY REACTIONS. Ann N Y Acad Sci. 1964 Aug 27;116:964–985. doi: 10.1111/j.1749-6632.1964.tb52562.x. [DOI] [PubMed] [Google Scholar]
  12. MILES A. A., MILES E. M. Vascular reactions to histamine, histamine-liberator and leukotaxine in the skin of guinea-pigs. J Physiol. 1952 Oct;118(2):228–257. doi: 10.1113/jphysiol.1952.sp004789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MOTA I., VUGMAN I. Action of compound 48/80 on the mast cells and histamine content of guinea-pig tissues. Br J Pharmacol Chemother. 1956 Sep;11(3):304–307. doi: 10.1111/j.1476-5381.1956.tb01070.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MOTA I., VUGMAN I. Effects of anaphylactic shock and compound 48/80 on the mast cells of the guinea pig lung. Nature. 1956 Mar 3;177(4505):427–429. doi: 10.1038/177427a0. [DOI] [PubMed] [Google Scholar]
  15. Osler A. G., Oliveira B., Shin H. S., Sandberg A. L. The fixation of guinea pig complement by gamma 1 and gamma 2 immunoglobulins. J Immunol. 1969 Jan;102(1):269–271. [PubMed] [Google Scholar]
  16. Parish W. E., Coombs R. R. Peripheral blood eosinophilia in guinea-pigs following implantation of anaphylactic guinea-pig and human lung. Br J Haematol. 1968 Apr;14(4):425–445. doi: 10.1111/j.1365-2141.1968.tb06994.x. [DOI] [PubMed] [Google Scholar]
  17. SAMTER M., KOFOED M. A., PIEPER W. A factor in lungs of anaphylactically shocked guinea pigs which can induce eosinophilia in normal animals. Blood. 1953 Dec;8(12):1078–1090. [PubMed] [Google Scholar]
  18. WARD P. A., COCHRANE C. G., MUELLER-EBERHARD H. J. THE ROLE OF SERUM COMPLEMENT IN CHEMOTAXIS OF LEUKOCYTES IN VITRO. J Exp Med. 1965 Aug 1;122:327–346. doi: 10.1084/jem.122.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ward P. A. Chemotaxis of human eosinophils. Am J Pathol. 1969 Jan;54(1):121–128. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES