Abstract
Using the approach described by Botchan, Topp, and Sambrook (Cell 9:269-287, 1976), we analyzed the organization of the integrated viral sequences in five clonal isolates from the same permissive, inducible cell line (Cyp line) transformed by the tsP155 mutant of polyoma virus. In all five clones, viral sequences were found that could be assigned to a common integration site, as they were joined to the cellular DNA in the same fashion in every instance. However, the sequences comprised between these points differed markedly from clone to clone, as if cell propagation had been accompanied by amplification or recombination or both within the viral insertion. When the clones were compared, no correlation could be found between the abundance, or the organization, of the integrated viral sequences and the amount, or the nature, of the free viral DNA molecules produced during induction. Altogether, our findings suggest that specific events, occurring during either the excision or the subsequent replication of the integrated viral sequences, are responsible for the predominant production of nondefective viral DNA molecules by permissive transformed cells, such as Cyp cells.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Basilico C., Gattoni S., Zouzias D., Valle G. D. Loss of integrated viral DNA sequences in polyomatransformed cells is associated with an active viral A function. Cell. 1979 Jul;17(3):645–659. doi: 10.1016/0092-8674(79)90272-1. [DOI] [PubMed] [Google Scholar]
- Basilico C., Zouzias D., Della-Valle G., Gattoni S., Colantuoni V., Fenton R., Dailey L. Integration and excision of polyoma virus genomes. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):611–620. doi: 10.1101/sqb.1980.044.01.064. [DOI] [PubMed] [Google Scholar]
- Birg F., Dulbecco R., Fried M., Kamen R. State and organization of polyoma virus DNA sequences in transformed rat cell lines. J Virol. 1979 Feb;29(2):633–648. doi: 10.1128/jvi.29.2.633-648.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Botchan M., Stringer J., Mitchison T., Sambrook J. Integration and excision of SV40 DNA from the chromosome of a transformed cell. Cell. 1980 May;20(1):143–152. doi: 10.1016/0092-8674(80)90242-1. [DOI] [PubMed] [Google Scholar]
- Botchan M., Topp W., Sambrook J. Studies on simian virus 40 excision from cellular chromosomes. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):709–719. doi: 10.1101/sqb.1979.043.01.079. [DOI] [PubMed] [Google Scholar]
- Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
- Bourgaux P., Delbecchi L., Yu K. K., Herring E., Bourgaux-Ramoisy D. A mouse embryo cell line carrying an inducible, temperature-sensitive, polyoma virus genome. Virology. 1978 Jul 15;88(2):348–360. doi: 10.1016/0042-6822(78)90291-x. [DOI] [PubMed] [Google Scholar]
- Colantuoni V., Dailey L., Basilico C. Amplification of integrated viral DNA sequences in polyoma virus-transformed cells. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3850–3854. doi: 10.1073/pnas.77.7.3850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delbecchi L., Gendron D., Bourgaux P. Inducible permissive cells transformed by a temperature-sensitive polyoma virus: superinfection does not allow excision of the resident viral genome. J Virol. 1981 Jul;39(1):196–206. doi: 10.1128/jvi.39.1.196-206.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gattoni S., Colantuoni V., Basilico C. Relationship between integrated and nonintegrated viral DNA in rat cells transformed by polyoma virus. J Virol. 1980 Jun;34(3):615–626. doi: 10.1128/jvi.34.3.615-626.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
- Lania L., Gandini-Attardi D., Griffiths M., Cooke B., De Cicco D., Fried M. The polyoma virus 100K large T-antigen is not required for the maintenance of transformation. Virology. 1980 Feb;101(1):217–232. doi: 10.1016/0042-6822(80)90497-3. [DOI] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prasad I., Zouzias D., Basilico C. State of the viral DNA in rat cells transformed by polyoma virus. I. Virus rescue and the presence of nonintergrated viral DNA molecules. J Virol. 1976 May;18(2):436–444. doi: 10.1128/jvi.18.2.436-444.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Sylla B. S., Bourgaux-Ramoisy D., Bourgaux P. Induction of viral DNA synthesis in clonal derivatives of a permissive cell line transformed by a temperature-sensitive polyoma virus. Virology. 1980 Jan 30;100(2):357–369. doi: 10.1016/0042-6822(80)90527-9. [DOI] [PubMed] [Google Scholar]
- Zouzias D., Prasad I., Basilico C. State of the viral DNA in rat cells transformed by polyma virus. II. Identification of the cells containing nonintegrated viral DNA and the effect of viral mutations. J Virol. 1977 Oct;24(1):142–150. doi: 10.1128/jvi.24.1.142-150.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van der Ploeg L. H., Flavell R. A. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues. Cell. 1980 Apr;19(4):947–958. doi: 10.1016/0092-8674(80)90086-0. [DOI] [PubMed] [Google Scholar]