Abstract
A crude membrane extract was made from a typed human spleen and half of the product was solubilized by proteolytic methods previously described. The remaining half was treated with starch stearate and sodium dodecylsulphate and the soluble product compared with the first for its HL-A specificity and behaviour on Sephadex G-200. The product of the starch stearate method had a molecular weight in excess of 200,000 and might be a membrane subunit.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aoki T., Hämmerling U., De Harven E., Boyse E. A., Old L. J. Antigenic structure of cell surfaces. An immunoferritin study of the occurrence and topography of H-2' theta, and TL alloantigens on mouse cells. J Exp Med. 1969 Nov 1;130(5):979–1001. doi: 10.1084/jem.130.5.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyse E. A., Miyazawa M., Aoki T., Old L. J. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc Lond B Biol Sci. 1968 Jun 11;170(1019):175–193. doi: 10.1098/rspb.1968.0032. [DOI] [PubMed] [Google Scholar]
- Boyse E. A., Old L. J., Stockert E. An approach to the mapping of antigens on the cell surface. Proc Natl Acad Sci U S A. 1968 Jul;60(3):886–893. doi: 10.1073/pnas.60.3.886. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyse E. A., Stockert E., Old L. J. Isoantigens of the H-2 and Tla loci of the mouse: interactions affecting their representation on thymocytes. J Exp Med. 1968 Jul 1;128(1):85–95. doi: 10.1084/jem.128.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies D. A., Manstone A. J., Viza D. C., Colombani J., Dausset J. Human transplantation antigens: the HL-A (Hu-1) system and its homology with the mouse H-2 system. Transplantation. 1968 Jul;6(4):571–586. [PubMed] [Google Scholar]
- Davies D. A. Mouse histocompatibility isoantigens derived from normal and from tumour cells. Immunology. 1966 Aug;11(2):115–125. [PMC free article] [PubMed] [Google Scholar]
- Davies D. A. The molecular individuality of different mouse H-2 histocompatibility specificities determined by single genotypes. Transplantation. 1969 Jul;8(1):51–70. doi: 10.1097/00007890-196907000-00007. [DOI] [PubMed] [Google Scholar]
- Hämmerling U., Westphal O. Synthesis and use of O-stearoyl polysaccharides in passive hemagglutination and hemolysis. Eur J Biochem. 1967 Mar;1(1):46–50. doi: 10.1007/978-3-662-25813-2_9. [DOI] [PubMed] [Google Scholar]
- REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimada A., Nathenson S. G. Solubilization of membrane H-2 isoantigens: chromatographic separation of specificities determined by a single H-2 genotype. Biochem Biophys Res Commun. 1967 Dec 29;29(6):828–833. doi: 10.1016/0006-291x(67)90294-x. [DOI] [PubMed] [Google Scholar]
- Summerell J. M., Davies D. A. Further characterization of soluble mouse and human transplantation antigens. Transplant Proc. 1969 Mar;1(1):479–482. [PubMed] [Google Scholar]
- Summerell J. M., Davies D. A. Physical properties of mouse H-2 transplantation alloantigens. Biochim Biophys Acta. 1970 Apr 28;207(1):92–104. doi: 10.1016/0005-2795(70)90139-x. [DOI] [PubMed] [Google Scholar]