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. 1971 Apr;8(4):573–580.

Characterization of precipitating antibodies to E. coli O antigen in infants and children with acute pyelonephritis*

L Å Hanson, J Holmgren, U Jodal, B Kaijser
PMCID: PMC1712955  PMID: 4995935

Abstract

Precipitating serum antibodies to Escherichia coli O antigens from some infants and children with acute pyelonephritis were studied.

These precipitins were largely resistant to reduction with 2-mercaptoethanol and were localized in the 7S fraction after gel filtration of the patient sera through Sephadex G-200. The precipitins were inhibited by anti-IgG serum, but not by anti-IgA or -IgM sera using a combined single and double diffusion method. The IgG nature of the precipitins investigated was also substantiated by immunofluorescence studies of precipitates formed by E. coli O antigens and the corresponding antibodies in double diffusion plates.

Possible reasons for the occurrence of IgG precipitins predominantly in patients with recurrent attacks of pyelonephritis are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergström T., Lincoln K., Orskov F., Orskov I., Winberg J. Studies of urinary tract infections in infancy and childhood. 8. Reinfection vs. relapse in recurrent urinary tract infections. Evaluation by means of identification of infecting organisms. J Pediatr. 1967 Jul;71(1):13–20. doi: 10.1016/s0022-3476(67)80224-5. [DOI] [PubMed] [Google Scholar]
  2. Hanson L. A., Holmgren J., Jodal U., Winberg J. Precipitating antibodies to E. coli O antigens: a suggested difference in the antibody response of infants and children with first and recurrent attacks of pyelonephritis. Acta Paediatr Scand. 1969 Sep;58(5):506–512. doi: 10.1111/j.1651-2227.1969.tb04752.x. [DOI] [PubMed] [Google Scholar]
  3. Holmgren J., Eggertsen G., Hanson L. A., Lincoln K. Immunodiffusion studies on Escherichia coli. 1. Identification of O, K and H antigens in an O6 strain. Acta Pathol Microbiol Scand. 1969;76(2):304–318. [PubMed] [Google Scholar]
  4. Holmgren J. Studies of methods for quantitation of agglutinins and precipitins to Escherichia coli O and K antigens. Int Arch Allergy Appl Immunol. 1970;37(5):480–494. doi: 10.1159/000230238. [DOI] [PubMed] [Google Scholar]
  5. Holmgren J. The antibody response in rabbits to E. coli O antigen. Int Arch Allergy Appl Immunol. 1970;37(5):546–559. doi: 10.1159/000230242. [DOI] [PubMed] [Google Scholar]
  6. Lindblad B. S., Ekengren K. The long term prognosis of non-obstructive urinary tract infection in infancy and childhood after the advent of sulphonamides. Acta Paediatr Scand. 1969 Jan;58(1):25–32. doi: 10.1111/j.1651-2227.1969.tb04325.x. [DOI] [PubMed] [Google Scholar]
  7. McGeachie J. Recurrent infection of the urinary tract: reinfection or recrudescence? Br Med J. 1966 Apr 16;1(5493):952–954. doi: 10.1136/bmj.1.5493.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SCHROHENLOHER R. E., KUNKEL H. G., TOMASI T. B. ACTIVITY OF DISSOCIATED AND REASSOCIATED 19S ANTI-GAMMA-GLOBULINS. J Exp Med. 1964 Dec 1;120:1215–1229. doi: 10.1084/jem.120.6.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SVEHAG S. E., MANDEL B. THE FORMATION AND PROPERTIES OF POLIOVIRUS-NEUTRALIZING ANTIBODY. I. 19S AND 7S ANTIBODY FORMATION: DIFFERENCES IN KINETICS AND ANTIGEN DOSE REQUIREMENT FOR INDUCTION. J Exp Med. 1964 Jan 1;119:1–19. doi: 10.1084/jem.119.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vosti K. L., Remington J. S. Host-parasite interaction in patients with infections due to Escherichia coli. 3. Physicochemical characterization of O-specific antibodies in serum and urine. J Lab Clin Med. 1968 Jul;72(1):71–84. [PubMed] [Google Scholar]
  11. WADSWORTH C. A slide microtechnique for the analysis of immune precipitates in gel. Int Arch Allergy Appl Immunol. 1957;10(6):355–360. doi: 10.1159/000228394. [DOI] [PubMed] [Google Scholar]
  12. WINBERG J., ANDERSEN H. J., HANSON L. A., LINCOLN K. STUDIES OF URINARY TRACT INFECTIONS IN INFANCY AND CHILDHOOD. I. ANTIBODY RESPONSE IN DIFFERENT TYPES OF URINARY TRACT INFECTIONS CAUSED BY COLIFORM BACTERIA. Br Med J. 1963 Aug 31;2(5356):524–527. doi: 10.1136/bmj.2.5356.524. [DOI] [PMC free article] [PubMed] [Google Scholar]

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