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. 1971 Apr;8(4):639–646.

Chemotaxis of polymorphonuclear leucocytes from germ-free rats and generation of chemotactic activity in germ-free rat sera

A Trippestad, T Midtvedt
PMCID: PMC1712967  PMID: 4930553

Abstract

Peritoneal exudates induced in germ-free rats by sodium caseinate contained substantially smaller numbers of polymorphonuclear leucocytes than did similar exudates from conventional rats. The chemotactic response of leucocytes from germ-free rats to various chemotactic agents was similar to the response of conventional rat leucocytes, when estimated in vitro by means of Boyden's technique. Furthermore, cytotaxins formed in sera from germ-free rats treated with antigen–antibody complexes or zymosan induced the same degree of conventional leucocyte migration as did cytotaxins formed in sera from conventional rats. Escherichia coli generated chemotactic activity in the presence of sera from germ-free rats, which was not less pronounced than the activity generated when sera from rats monocontaminated with the same microbe were used. Apparently, sera from germ-free rats contained all the factors necessary for cytotaxin formation by the strain of bacteria used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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