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. 1971 Apr;8(4):563–572.

Hypersensitivity reactions to acetylsalicylic acid. I. Detection of antibodies in human sera using acetylsalicylic acid attached to proteins through the carboxyl group

H E Amos, D V Wilson, M J Taussig, S J Carlton
PMCID: PMC1712969  PMID: 4252628

Abstract

Aspirin–protein conjugates (aspirin coupled to rabbit anti-human `O' antibody and to anti-bacteriophage-Fab') were prepared by a method which links aspirin through its carboxyl group. The protein conjugates were used to raise specific aspirin antibodies in guinea-pigs. Such antibodies helped to characterize the other preparations which were subsequently used to test suspected hypersensitive sera for aspirin antibodies. Although an antibody of aspirin specificity was detected it could not be related to the clinical hypersensitivity state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COOMBS R. R., HOWARD A. N., MYNORS L. S. A serological procedure theoretically capable of detecting incomplete or non-precipitating antibodies to soluble protein antigens. Br J Exp Pathol. 1953 Oct;34(5):525–534. [PMC free article] [PubMed] [Google Scholar]
  3. Coombs R. R., Hunter A., Jonas W. E., Bennich H., Johannson S. G., Panzani R. Detection of IgE(IgND) specific antibody (probably reagin) to castor-bean allergen by the red-cell-linked antigen-antiglobulin reaction. Lancet. 1968 May 25;1(7552):1115–1118. doi: 10.1016/s0140-6736(68)90186-4. [DOI] [PubMed] [Google Scholar]
  4. Haimovich J., Sela M. Protein-bacteriophage conjugates: application in detection of antibodies and antigens. Science. 1969 Jun 13;164(3885):1279–1280. doi: 10.1126/science.164.3885.1279. [DOI] [PubMed] [Google Scholar]
  5. MATHEWS K. P., LOVELL R. G., SHELDON J. M. The problem of aspirin allergy with a report on skin testing with salicylate-containing human sera. J Lab Clin Med. 1950 Sep;36(3):416–421. [PubMed] [Google Scholar]
  6. Mäkelä O. Assay of anti-hapten antibody with the aid of hapten-coupled bacteriophage. Immunology. 1966 Jan;10(1):81–86. [PMC free article] [PubMed] [Google Scholar]
  7. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  8. Taussig M. J. Bacteriophage linked assays for antibodies to protein antigens. Immunology. 1970 Mar;18(3):323–330. [PMC free article] [PubMed] [Google Scholar]
  9. WEINER L. M., ROSENBLATT M., HOWES H. A. THE DETECTION OF HUMORAL ANTIBODIES DIRECTED AGAINST SALICYLATES IN HYPERSENSITIVE STATES. J Immunol. 1963 May;90:788–792. [PubMed] [Google Scholar]

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