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. 1971 May;8(5):685–700.

Localization of Gm markers to different molecular regions of the Fc fragment

J B Natvig, M W Turner
PMCID: PMC1712978  PMID: 4103295

Abstract

pFc' fragments (corresponding to homology region Cγ3) and smaller subfragments produced by trypsin and papain have been studied by haemagglutination inhibition techniques for the presence of a range of Fcγ allotypic markers. The markers which were identified in the pFc' fragment included Gm(a), (x), (b0), (b3), (b4), (b5), (c3), (c5) and `non a'. In addition, an IgG 4 marker which possibly occupies the same molecular region as the Gm(a) and `non a' antigens was detected in pFc' fragments from IgG 4 proteins.

Limited tryptic cleavage of pFc' fragment resulting in the loss of approximately seven N-terminal residues had no effect on the antigenic expression of the genetic markers. More extensive degradation of pFc' fragments with papain yielded Fc'-like fragments which are presumed to lack a C-terminal tridecapeptide as well as eight or nine N-terminal residues. In these fragments antigenic expression of Gm(a), (x) and `non a' was lost, but Gm(b0), (b3), (b4) and (b5) antigens were still detectable.

The other allotypic markers of the Fc region (Gm(n), (b1), (g) and `non g') were not detected in any of the fragments investigated and it is probable that they are in homology region Cγ2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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