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. 1971 Nov;9(5):603–609.

In vitro cytotoxicity of lymphocytes from patients with `acquired' and sex-linked agammaglobulinaemia

E Lieber, S D Douglas, H H Fudenberg
PMCID: PMC1713062  PMID: 5169336

Abstract

The in vitro cytotoxicity for fowl erythrocytes of circulating lymphocytes from nine patients with `acquired' and three patients with sex-linked agammaglobulinaemia was tested by release of sodium chromate (51Cr) after 42 hr of culture in the presence of phytohaemagglutinin (PHA). The lymphocyte cytotoxicity of three patients with `acquired' agammaglobulinaemia was low, but that of the other six and of the three patients with sex-linked agammaglobulinaemia were normal. All the `acquired' agammaglobulinaemic patients had markedly diminished thymidine uptake in response to PHA at 3 days, but a normal response at 7–10 days, but the three boys with sex-linked agammaglobulinaemia showed a normal response. The findings further demonstrate the heterogeneity of lymphocyte abnormalities in patients with agammaglobulinaemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billingham R. E. Lymphocyte response to antigens. B. Efferent side of sensitization arc. Evidence for cytopathic effects of lymphocytes. Transplantation. 1967 Jul;5(4 Suppl):976–985. doi: 10.1097/00007890-196707001-00029. [DOI] [PubMed] [Google Scholar]
  2. Douglas S. D., Goldberg L. S., Fudenberg H. H. Clinical, serologic and leukocyte function studies on patients with idiopathic "acquired" agammaglobulinemia and their families. Am J Med. 1970 Jan;48(1):48–53. doi: 10.1016/0002-9343(70)90097-5. [DOI] [PubMed] [Google Scholar]
  3. Douglas S. D., Kamin R. M., Fudenberg H. H. Human lymphocyte response to phytomitogens in vitro: normal, agammaglobulinemic and paraproteinemic individuals. J Immunol. 1969 Dec;103(6):1185–1195. [PubMed] [Google Scholar]
  4. FUDENBERG H. H., HIRSCHHORN K. AGAMMAGLOBULINEMIA: THE FUNDAMENTAL DEFECT. Science. 1964 Aug 7;145(3632):611–612. doi: 10.1126/science.145.3632.611. [DOI] [PubMed] [Google Scholar]
  5. GOOD R. A., VARCO R. L., AUST J. B., ZAK S. J. Transplantation studies in patients with agammaglobulinemia. Ann N Y Acad Sci. 1957 Mar 22;64(5):882-924; discussion, 924-8. doi: 10.1111/j.1749-6632.1957.tb52482.x. [DOI] [PubMed] [Google Scholar]
  6. Holm G., Perlmann P. Cytotoxic potential of stimulated human lymphocytes. J Exp Med. 1967 Apr 1;125(4):721–736. doi: 10.1084/jem.125.4.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holm G., Perlmann P., Johansson B. Impaired phytohaemagglutinin-induced cytotoxicity in vitro of lymphocytes from patients with Hodgkin's disease or chronic lymphatic leukaemia. Clin Exp Immunol. 1967 May;2(3):351–360. [PMC free article] [PubMed] [Google Scholar]
  8. Holm G., Perlmann P. Quantitative studies on phytohaemagglutinin-induced cytotoxicity by human lymphocytes against homologous cells in tissue culture. Immunology. 1967 May;12(5):525–536. [PMC free article] [PubMed] [Google Scholar]
  9. Lundgren G., Möller G. Non-specific induction of cytotoxicity in normal human lymphocytes in vitro: studies of mechanism and specificity of the reaction. Clin Exp Immunol. 1969 Apr;4(4):435–452. [PMC free article] [PubMed] [Google Scholar]
  10. Perlmann P., Perlmann H., Holm G. Cytotoxic action of stimulated lymphocytes on allogenic and autologous erythrocytes. Science. 1968 Apr 19;160(3825):306–309. doi: 10.1126/science.160.3825.306. [DOI] [PubMed] [Google Scholar]

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