Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1981 Sep;39(3):968–972. doi: 10.1128/jvi.39.3.968-972.1981

Genome analysis of MG virus, a human papovavirus.

M M Pater, A Pater, G di Mayorca
PMCID: PMC171333  PMID: 6270363

Abstract

The genome of MG virus, a variant of the human papovavirus BK virus, consists of two molecules, M1 and M2, M1 and M2 have deletions which correspond to 0.33 to 0.55 map unit and 0.77 to 0.85 map unit, respectively, from the EcoRI site on the BK virus genome. Restriction enzyme analysis of the DNAs of these viruses revealed many alterations in both DNA species. Both M1 and M2 DNAs have three recognition sites for EcoRI. M1 has a single recognition site for HindIII and five sites for PvuII. M2 has a single recognition site for PuvII and three sites for HindIII. The sites of these and several other restriction enzymes on each DNA molecule were mapped after the cloning of M1 and M2 DNAs into pBR322 at their unique HindIII and PvuII sites, respectively.

Full text

PDF
968

Images in this article

969Image
on p.969
969Image
on p.969
972Image
on p.972

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  2. Dougherty R. M., DiStefano H. S. Isolation and characterization of a papovavirus from human urine. Proc Soc Exp Biol Med. 1974 Jun;146(2):481–487. doi: 10.3181/00379727-146-38131. [DOI] [PubMed] [Google Scholar]
  3. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  4. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lecatsas G., Prozesky O. W., van Wyk J., Els H. J. Papova virus in urine after renal transplantation. Nature. 1973 Feb 2;241(5388):343–344. doi: 10.1038/241343a0. [DOI] [PubMed] [Google Scholar]
  6. Lecatsas G., Schoub B. D., Prozesky O. W. Development of a new human polyoma virus strain (MG). Arch Virol. 1976;51(4):327–333. doi: 10.1007/BF01317936. [DOI] [PubMed] [Google Scholar]
  7. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  8. Miao R., Dougherty R. M. Characterization of human papovavirus RFV: comparison with SV40 and BKV. J Gen Virol. 1977 Apr;35(1):67–75. doi: 10.1099/0022-1317-35-1-67. [DOI] [PubMed] [Google Scholar]
  9. Pater A., Pater M. M., di Mayorca G. Arrangement of the genome of the human papovavirus RF virus. J Virol. 1980 Nov;36(2):480–487. doi: 10.1128/jvi.36.2.480-487.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pater M. M., Pater A., di Mayorca G. Comparative analysis of GS and BK virus genomes. J Virol. 1979 Oct;32(1):220–225. doi: 10.1128/jvi.32.1.220-225.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pauw W., Choufoer J. Isolation of a variant of BK virus with altered restriction endonuclease pattern. Arch Virol. 1978;57(1):35–42. doi: 10.1007/BF01315635. [DOI] [PubMed] [Google Scholar]
  12. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  13. Seif I., Khoury G., Dhar R. The genome of human papovavirus BKV. Cell. 1979 Dec;18(4):963–977. doi: 10.1016/0092-8674(79)90209-5. [DOI] [PubMed] [Google Scholar]
  14. Takemoto K. K., Rabson A. S., Mullarkey M. F., Blaese R. M., Garon C. F., Nelson D. Isolation of papovavirus from brain tumor and urine of a patient with Wiskott-Aldrich syndrome. J Natl Cancer Inst. 1974 Nov;53(5):1205–1207. doi: 10.1093/jnci/53.5.1205. [DOI] [PubMed] [Google Scholar]
  15. Wright P. J., Bernhardt G., Major E. O., di Mayorca G. Comparison of the serology, transforming ability, and polypeptide composition of human papovaviruses isolated from urine. J Virol. 1976 Mar;17(3):762–775. doi: 10.1128/jvi.17.3.762-775.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang R. C., Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science. 1979 Oct 26;206(4417):456–462. doi: 10.1126/science.228391. [DOI] [PubMed] [Google Scholar]
  17. Yang R. C., Wu R. Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40. Nucleic Acids Res. 1979 Oct 10;7(3):651–668. doi: 10.1093/nar/7.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES