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. 1981 Aug;39(2):348–358. doi: 10.1128/jvi.39.2.348-358.1981

Specific Sindbis virus-coded function for minus-strand RNA synthesis.

D L Sawicki, S G Sawicki, S Keränen, L Kääriäinen
PMCID: PMC171343  PMID: 7277580

Abstract

The synthesis of minus-strand RNA was studied in cell cultures infected with the heat-resistant strain of Sindbis virus and with temperature-sensitive (ts) belonging to complementation groups A, B, F, and G, all of which exhibited an RNA-negative (RNA-) phenotype when infection was initiated and maintained at 39 degrees C, the nonpermissive temperature. When infected cultures were shifted from 28 degrees C (the permissive temperature) to 39 degrees C at 3 h postinfection, the synthesis of viral minus-strand RNA ceased in cultures infected with ts mutants of complementation groups B and F, but continued in cultures infected with the parental virus and mutans of complementation groups A and G. In cultures infected with ts11 of complementation group B, the synthesis of viral minus-strand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h after the shift to 39 degrees C. However, when ts11-infected cultures were returned to 28 degrees C 1 h after the shift to 39 degrees C, the synthesis of viral minus-strand RNA resumed, and the rate of viral RNA synthesis increased. The recovery of minus-strand synthesis translation of new proteins. We conclude that at least one viral function is required for alphavirus minus-strand synthesis that is not required for plus-strand synthesis. In cultures infected with ts6 of complementation group F, the syntheses of both viral plus-strand and minus-strand RNAs were drastically reduced after the shift to 39 degrees C. Since ts6 failed to synthesize both plus-strand and minus-strand RNAs after the shift to 39 degrees C, at least one common viral component appears to be required for the synthesis of both minus-strand and plus-strand RNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenthal T., Carmichael G. G. RNA replication: function and structure of Qbeta-replicase. Annu Rev Biochem. 1979;48:525–548. doi: 10.1146/annurev.bi.48.070179.002521. [DOI] [PubMed] [Google Scholar]
  2. Bracha M., Leone A., Schlesinger M. J. Formation of a Sindbis virus nonstructural protein and its relation of 42S mRNA function. J Virol. 1976 Dec;20(3):612–620. doi: 10.1128/jvi.20.3.612-620.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brzeski H., Kennedy S. I. Synthesis of Sindbis virus nonstructural polypeptides in chicken embryo fibroblasts. J Virol. 1977 May;22(2):420–429. doi: 10.1128/jvi.22.2.420-429.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burge B. W., Pfefferkorn E. R. Complementation between temperature-sensitive mutants of Sindbis virus. Virology. 1966 Oct;30(2):214–223. doi: 10.1016/0042-6822(66)90097-3. [DOI] [PubMed] [Google Scholar]
  5. Burge B. W., Pfefferkorn E. R. Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology. 1966 Oct;30(2):204–213. doi: 10.1016/0042-6822(66)90096-1. [DOI] [PubMed] [Google Scholar]
  6. Dasgupta A., Zabel P., Baltimore D. Dependence of the activity of the poliovirus replicase on the host cell protein. Cell. 1980 Feb;19(2):423–429. doi: 10.1016/0092-8674(80)90516-4. [DOI] [PubMed] [Google Scholar]
  7. Dmitrieva T. M., Shcheglova M. V., Agol V. I. Inhibition of activity of encephalomyocarditis virus-induced RNA polymerase by antibodies against cellular components. Virology. 1979 Jan 30;92(2):271–277. doi: 10.1016/0042-6822(79)90131-4. [DOI] [PubMed] [Google Scholar]
  8. Dubin D. T., Timko K., Gillies S., Stollar V. The extreme 5'-terminal sequences of sindbis virus 26 and 42 S RNA. Virology. 1979 Oct 15;98(1):131–141. doi: 10.1016/0042-6822(79)90532-4. [DOI] [PubMed] [Google Scholar]
  9. Flanegan J. B., Petterson R. F., Ambros V., Hewlett N. J., Baltimore D. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus. Proc Natl Acad Sci U S A. 1977 Mar;74(3):961–965. doi: 10.1073/pnas.74.3.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frey T. K., Strauss J. H. Replication of Sindbis virus. VI. Poly(A) and poly(U) in virus-specific RNA species. Virology. 1978 May 15;86(2):494–506. doi: 10.1016/0042-6822(78)90088-0. [DOI] [PubMed] [Google Scholar]
  12. Friedman R. M., Grimley P. M. Inhibition of arbovirus assembly by cycloheximide. J Virol. 1969 Sep;4(3):292–299. doi: 10.1128/jvi.4.3.292-299.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fuller F. J., Marcus P. I. Sindbis virus. I. Gene order of translation in vivo. Virology. 1980 Dec;107(2):441–451. doi: 10.1016/0042-6822(80)90311-6. [DOI] [PubMed] [Google Scholar]
  14. Keränen S., Käriäinen L. Functional defects of RNA-negative temperature-sensitive mutants of Sindbis and Semliki Forest viruses. J Virol. 1979 Oct;32(1):19–29. doi: 10.1128/jvi.32.1.19-29.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Käriäinen L., Söderlund H. Structure and replication of alpha-viruses. Curr Top Microbiol Immunol. 1978;82:15–69. doi: 10.1007/978-3-642-46388-4_2. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Nomoto A., Detjen B., Pozzatti R., Wimmer E. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature. 1977 Jul 21;268(5617):208–213. doi: 10.1038/268208a0. [DOI] [PubMed] [Google Scholar]
  18. Pettersson R. F., Söderlund H., Käriäinen L. The nucleotide sequences of the 5'-terminal T1 oligonucleotides of Semliki-Forest-virus 42-S and 26-S RNAs are different. Eur J Biochem. 1980 Apr;105(3):435–443. doi: 10.1111/j.1432-1033.1980.tb04518.x. [DOI] [PubMed] [Google Scholar]
  19. Sawicki D. L., Gomatos P. J. Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA. J Virol. 1976 Nov;20(2):446–464. doi: 10.1128/jvi.20.2.446-464.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sawicki D. L., Kaariainen L., Lambek C., Gomatos P. J. Mechanism for control of synthesis of Semliki Forest virus 26S and 42s RNA. J Virol. 1978 Jan;25(1):19–27. doi: 10.1128/jvi.25.1.19-27.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sawicki D. L., Sawicki S. G. Short-lived minus-strand polymerase for Semliki Forest virus. J Virol. 1980 Apr;34(1):108–118. doi: 10.1128/jvi.34.1.108-118.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scheele C. M., Pfefferkorn E. R. Inhibition of interjacent ribonucleic acid (26S) synthesis in cells infected by Sindbis virus. J Virol. 1969 Aug;4(2):117–122. doi: 10.1128/jvi.4.2.117-122.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spector D. H., Baltimore D. Polyadenylic acid on poliovirus RNA IV. Poly(U) in replicative intermediate and double-stranded RNA. Virology. 1975 Oct;67(2):498–505. doi: 10.1016/0042-6822(75)90450-x. [DOI] [PubMed] [Google Scholar]
  24. Strauss E. G., Lenches E. M., Strauss J. H. Mutants of sindbis virus. I. Isolation and partial characterization of 89 new temperature-sensitive mutants. Virology. 1976 Oct 1;74(1):154–168. doi: 10.1016/0042-6822(76)90137-9. [DOI] [PubMed] [Google Scholar]
  25. Waite M. R. Protein synthesis directed by an RNA temperature-sensitive mutant of Sindbis virus. J Virol. 1973 Feb;11(2):198–206. doi: 10.1128/jvi.11.2.198-206.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wengler G. Studies on the synthesis of viral RNA-polymerase-template complexes in BHK 21 cells infected with Semliki Forest virus. Virology. 1975 Jul;66(1):322–326. doi: 10.1016/0042-6822(75)90202-0. [DOI] [PubMed] [Google Scholar]
  27. Wengler G., Wengler G., Gross H. S. Replicative form of Semliki Forest virus RNA contains an unpaired guanosine. Nature. 1979 Dec 13;282(5740):754–756. doi: 10.1038/282754a0. [DOI] [PubMed] [Google Scholar]
  28. Yogo Y., Teng M. H., Wimmer E. Poly(U) in poliovirus minus RNA is 5'-terminal. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1101–1109. doi: 10.1016/s0006-291x(74)80397-9. [DOI] [PubMed] [Google Scholar]
  29. Yogo Y., Wimmer E. Poly (A) and poly (U) in poliovirus double stranded RNA. Nat New Biol. 1973 Apr 11;242(119):171–174. doi: 10.1038/newbio242171a0. [DOI] [PubMed] [Google Scholar]

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