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. 1989 Feb;33(2):242–244. doi: 10.1128/aac.33.2.242

Macrolide accumulation by Bacteroides fragilis ATCC 25285.

Y Muto 1, K Bandoh 1, K Watanabe 1, N Katoh 1, K Ueno 1
PMCID: PMC171466  PMID: 2719467

Abstract

The accumulation of macrolide antibiotics in Bacteroides fragilis ATCC 25285 was increased in the order erythromycin, josamycin, and rokitamycin, depending on hydrophobicity. The half-times of efflux were also prolonged in the same order. Furthermore, MICs of the antibiotics were correlated with the extent of hydrophobicity. These findings suggest that the macrolide antibiotics are accumulated in B. fragilis by means of their hydrophobic properties, and the efficient accumulation of the drugs may explain the susceptibility of this gram-negative bacterium to macrolides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryan L. E., Kowand S. K., Van Den Elzen H. M. Mechanism of aminoglycoside antibiotic resistance in anaerobic bacteria: Clostridium perfringens and Bacteroides fragilis. Antimicrob Agents Chemother. 1979 Jan;15(1):7–13. doi: 10.1128/aac.15.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dunn W. J., 3rd, Hansch C. Chemicobiological interactions and the use of partition coefficients in their correlation. Chem Biol Interact. 1974 Aug;9(2):75–95. doi: 10.1016/0009-2797(74)90001-5. [DOI] [PubMed] [Google Scholar]
  3. Fayolle F., Privitera G., Sebald M. Tetracycline transport in Bacteroides fragilis. Antimicrob Agents Chemother. 1980 Oct;18(4):502–505. doi: 10.1128/aac.18.4.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hancock R. E. Alterations in outer membrane permeability. Annu Rev Microbiol. 1984;38:237–264. doi: 10.1146/annurev.mi.38.100184.001321. [DOI] [PubMed] [Google Scholar]
  5. Hofstad T., Sveen K., Dahlén G. Chemical composition, serological reactivity and endotoxicity of lipopolysaccharides extracted in different ways from Bacteroides fragilis, Bacteroides melaninogenicus and Bacteroides oralis. Acta Pathol Microbiol Scand B. 1977 Aug;85(4):262–270. doi: 10.1111/j.1699-0463.1977.tb01972.x. [DOI] [PubMed] [Google Scholar]
  6. Kasper D. L. Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1976 Jul;134(1):59–66. doi: 10.1093/infdis/134.1.59. [DOI] [PubMed] [Google Scholar]
  7. Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
  8. Kesado T., Watanabe K., Asahi Y., Isono M., Ueno K. Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis. Antimicrob Agents Chemother. 1984 Jan;25(1):131–133. doi: 10.1128/aac.25.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mao J. C., Putterman M. Accumulation in gram-postive and gram-negative bacteria as a mechanism of resistance to erythromycin. J Bacteriol. 1968 Mar;95(3):1111–1117. doi: 10.1128/jb.95.3.1111-1117.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mao J. C., Wiegand R. G. Mode of action of macrolides. Biochim Biophys Acta. 1968 Apr 22;157(2):404–413. doi: 10.1016/0005-2787(68)90094-4. [DOI] [PubMed] [Google Scholar]
  11. Nikaido H., Nakae T. The outer membrane of Gram-negative bacteria. Adv Microb Physiol. 1979;20:163–250. doi: 10.1016/s0065-2911(08)60208-8. [DOI] [PubMed] [Google Scholar]
  12. Nikaido H. Outer membrane of Salmonella typhimurium. Transmembrane diffusion of some hydrophobic substances. Biochim Biophys Acta. 1976 Apr 16;433(1):118–132. doi: 10.1016/0005-2736(76)90182-6. [DOI] [PubMed] [Google Scholar]
  13. Nikaido H., Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. doi: 10.1128/mr.49.1.1-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolf-Watz H., Elmros T., Normark S., Bloom G. D. Cell envelope of Neisseria gonorrhoeae: outer membrane and peptidoglycan composition of penicillin-sensitive and-resistant strains. Infect Immun. 1975 Jun;11(6):1332–1341. doi: 10.1128/iai.11.6.1332-1341.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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