Skip to main content
PMC home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1989 Feb;44(2):282–287.

Loss of heterozygosity in a gene coding for a thyroid hormone receptor in lung cancers

François Leduc, Hiltrud Brauch, Camile Hajj, Alexander Dobrovic, Frederick Kaye, Adi Gazdar, J William Harbour, Olive S Pettengill, George D Sorenson, A van den Berg, K Kok, Barbara Campling, François Paquin, W E C Bradley, Berton Zbar, John Minna, Charles Buys, Joseph Ayoub
PMCID: PMC1715394  PMID: 2536219

Abstract

The ERBAβ gene codes for a DNA-binding thyroid hormone receptor (THR) and maps to chromosome 3p21-p25, overlapping a 3p deletion characterizing small-cell lung carcinoma (SCLC). A DNA clone detecting an RFLP at the ERBAβ locus has been used to probe a large number of lung .tumors. Virtually all SCLC had lost heterozygosity, showing that the 3p deletion in SCLC includes this gene. A substantial but smaller proportion of non-small-cell carcinomas had lost heterozygosity at ERBAβ. Among all non-small-cell tumors some had lost heterozygosity at the proximal locus DNF15S2 (band 3p21) but not at ERBAβ, whereas none were found where the reverse was true. Therefore, the locus which plays a role in non-small-cell tumorigenesis probably lies closer to DNF15S2 than to ERBAβ and is almost certainly not the latter.

Full text

PDF
282

Images in this article

284Figure 1
on p.284
286Figure 2
on p.286

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brauch H., Johnson B., Hovis J., Yano T., Gazdar A., Pettengill O. S., Graziano S., Sorenson G. D., Poiesz B. J., Minna J. Molecular analysis of the short arm of chromosome 3 in small-cell and non-small-cell carcinoma of the lung. N Engl J Med. 1987 Oct 29;317(18):1109–1113. doi: 10.1056/NEJM198710293171803. [DOI] [PubMed] [Google Scholar]
  2. Carritt B., Welch H. M., Parry-Jones N. J. Sequences homologous to the human D1S1 locus present on human chromosome 3. Am J Hum Genet. 1986 Apr;38(4):428–436. [PMC free article] [PubMed] [Google Scholar]
  3. Di Liegro I., Savettieri G., Cestelli A. Cellular mechanism of action of thyroid hormones. Differentiation. 1987;35(3):165–175. doi: 10.1111/j.1432-0436.1987.tb00165.x. [DOI] [PubMed] [Google Scholar]
  4. Dobrovic A., Houle B., Belouchi A., Bradley W. E. erbA-related sequence coding for DNA-binding hormone receptor localized to chromosome 3p21-3p25 and deleted in small cell lung carcinoma. Cancer Res. 1988 Feb 1;48(3):682–685. [PubMed] [Google Scholar]
  5. Gareau J. L., Houle B., Leduc F., Bradley W. E., Dobrovic A. A frequent HindIII RFLP on chromosome 3p21-25 detected by a genomic erbA beta sequence. Nucleic Acids Res. 1988 Feb 11;16(3):1223–1223. doi: 10.1093/nar/16.3.1223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kok K., Osinga J., Carritt B., Davis M. B., van der Hout A. H., van der Veen A. Y., Landsvater R. M., de Leij L. F., Berendsen H. H., Postmus P. E. Deletion of a DNA sequence at the chromosomal region 3p21 in all major types of lung cancer. Nature. 1987 Dec 10;330(6148):578–581. doi: 10.1038/330578a0. [DOI] [PubMed] [Google Scholar]
  7. Naylor S. L., Johnson B. E., Minna J. D., Sakaguchi A. Y. Loss of heterozygosity of chromosome 3p markers in small-cell lung cancer. Nature. 1987 Oct 1;329(6138):451–454. doi: 10.1038/329451a0. [DOI] [PubMed] [Google Scholar]
  8. Rey J. A., Bello M. J., de Campos J. M., Kusak M. E., Moreno S., Benítez J. Deletion 3p in two lung adenocarcinomas metastatic to the brain. Cancer Genet Cytogenet. 1987 Apr;25(2):355–360. doi: 10.1016/0165-4608(87)90197-x. [DOI] [PubMed] [Google Scholar]
  9. Weinberger C., Thompson C. C., Ong E. S., Lebo R., Gruol D. J., Evans R. M. The c-erb-A gene encodes a thyroid hormone receptor. Nature. 1986 Dec 18;324(6098):641–646. doi: 10.1038/324641a0. [DOI] [PubMed] [Google Scholar]
  10. Whang-Peng J., Kao-Shan C. S., Lee E. C., Bunn P. A., Carney D. N., Gazdar A. F., Minna J. D. Specific chromosome defect associated with human small-cell lung cancer; deletion 3p(14-23). Science. 1982 Jan 8;215(4529):181–182. doi: 10.1126/science.6274023. [DOI] [PubMed] [Google Scholar]
  11. Whang-Peng J., Lee E., Knutsen T., Solanki D. Dicentric isochromosome for the long arm of chromosome #17, dic i(17q), in a patient with chronic myelogenous leukemia (CML). Cancer Genet Cytogenet. 1981 Apr;3(3):233–236. doi: 10.1016/0165-4608(81)90089-3. [DOI] [PubMed] [Google Scholar]
  12. Yokota J., Wada M., Shimosato Y., Terada M., Sugimura T. Loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9252–9256. doi: 10.1073/pnas.84.24.9252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zech L., Bergh J., Nilsson K. Karyotypic characterization of established cell lines and short-term cultures of human lung cancers. Cancer Genet Cytogenet. 1985 Feb 15;15(3-4):335–347. doi: 10.1016/0165-4608(85)90178-5. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES