Figure 2.

Screening of the most effective targeting site for the Tiam1 gene by real-time PCR and Western blot analysis. (A) Quantification of Tiam1 mRNA expression in cells of different interference sites relative to controls (M, pLenti6/U6 Mock virus-transduced cells), as detected by real-time PCR. (B) Tiam1 protein expression in cells of different interference sites, as detected by Western blot analysis. Lane L shows that a greater degree of Lamin A/C knockdown was observed after transduction with a lentivirus containing the human Lamin A/C shRNA-expressing cassette. Lane B shows that lentiviral vectors were effective and that site B was the most effective construct.