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. 1997 May;76(5):425–427. doi: 10.1136/adc.76.5.425

Gastrointestinal handling of [1-13C]palmitic acid in healthy controls and patients with cystic fibrosis

J Murphy 1, A Jones 1, M Stolinski 1, S Wootton 1
PMCID: PMC1717187  PMID: 9196358

Abstract

Accepted 2 January 1997


AIM—To examine the gastrointestinal handling of [1-13C]palmitic acid given as the free acid by measuring the excretion of 13C label in stool in 16 healthy children and 11 patients with cystic fibrosis on their habitual enzyme replacement treatment.
METHODS—After an overnight fast, each child ingested 10 mg/kg body weight [1-13C]palmitic acid with a standardised test meal of low natural 13C abundance. A stool sample was collected before the test and all stools were collected thereafter for a period of up to five days. The total enrichment of 13C in stool and the species bearing the 13C label was measured using isotope ratio mass spectrometry.
RESULTS—The proportion of administered 13C label excreted in stool was 24.0% (range 10.7-64.9%) in healthy children and only 4.4% (range 1.2-11.6%) in cystic fibrosis patients. The enrichment of 13C in stool was primarily restricted to the species consumed by the subjects (that is as palmitic acid).
CONCLUSION—There does not appear to be a specific defect in the absorption of [1-13C]palmitic acid in patients with cystic fibrosis. The reasons why cystic fibrosis patients appear to absorb more of this saturated fatty acid than healthy children is not clear and requires further investigation.



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Selected References

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  1. Baxter P. S., Dickson J. A., Variend S., Taylor C. J. Intestinal disease in cystic fibrosis. Arch Dis Child. 1988 Dec;63(12):1496–1497. doi: 10.1136/adc.63.12.1496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brink E. J., Haddeman E., de Fouw N. J., Weststrate J. A. Positional distribution of stearic acid and oleic acid in a triacylglycerol and dietary calcium concentration determines the apparent absorption of these fatty acids in rats. J Nutr. 1995 Sep;125(9):2379–2387. doi: 10.1093/jn/125.9.2379. [DOI] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Frase L. L., Strickland A. D., Kachel G. W., Krejs G. J. Enhanced glucose absorption in the jejunum of patients with cystic fibrosis. Gastroenterology. 1985 Feb;88(2):478–484. doi: 10.1016/0016-5085(85)90510-4. [DOI] [PubMed] [Google Scholar]
  5. Murphy J. L., Jones A., Brookes S., Wootton S. A. The gastrointestinal handling and metabolism of [1-13C]palmitic acid in healthy women. Lipids. 1995 Apr;30(4):291–298. doi: 10.1007/BF02536035. [DOI] [PubMed] [Google Scholar]
  6. Murphy J. L., Wootton S. A., Bond S. A., Jackson A. A. Energy content of stools in normal healthy controls and patients with cystic fibrosis. Arch Dis Child. 1991 Apr;66(4):495–500. doi: 10.1136/adc.66.4.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tadayyon B., Lutwak L. Interrelationship of triglycerides with calcium, magnesium and phosphorus in the rat. J Nutr. 1969 Feb;97(2):246–254. doi: 10.1093/jn/97.2.246. [DOI] [PubMed] [Google Scholar]
  8. Wootton S. A., Murphy J. L., Bond S. A., Ellis J. E., Jackson A. A. Energy balance and growth in cystic fibrosis. J R Soc Med. 1991;84 (Suppl 18):22–27. [PMC free article] [PubMed] [Google Scholar]
  9. Zentler-Munro P. L. Cystic fibrosis--a gastroenterological cornucopia. Gut. 1987 Nov;28(11):1531–1547. doi: 10.1136/gut.28.11.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]

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