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. 1990 May;34(5):924–927. doi: 10.1128/aac.34.5.924

Cloning and heterospecific expression of the resistance determinant vanA encoding high-level resistance to glycopeptides in Enterococcus faecium BM4147.

A Brisson-Noël 1, S Dutka-Malen 1, C Molinas 1, R Leclercq 1, P Courvalin 1
PMCID: PMC171723  PMID: 2360831

Abstract

Fragments of plasmid pIP816, which confers high-level glycopeptide resistance in Enterococcus faecium BM4147, were cloned into a conjugative gram-negative-gram-positive shuttle vector. The resulting hybrids were transferred by conjugation from Escherichia coli to Enterococcus faecalis and Bacillus thuringiensis. A 4-kilobase EcoRI fragment from pIP816 was found to confer vancomycin resistance in these hosts but not in E. coli or Bacillus subtilis.

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Selected References

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