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. 1990 Jun;34(6):1075–1078. doi: 10.1128/aac.34.6.1075

Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs.

S Merali 1, Y Zhang 1, D Sloan 1, S Meshnick 1
PMCID: PMC171761  PMID: 2203302

Abstract

A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 14 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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