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. 1990 Jun;34(6):1232–1236. doi: 10.1128/aac.34.6.1232

Eradication of Helicobacter mustelae from the ferret stomach: an animal model of Helicobacter (Campylobacter) pylori chemotherapy.

G Otto 1, J G Fox 1, P Y Wu 1, N S Taylor 1
PMCID: PMC171790  PMID: 2393285

Abstract

Colonization of the ferret stomach by Helicobacter mustelae has been suggested as a possible animal model for Helicobacter pylori-associated gastroduodenal disease of humans. Our study was designed to determine whether antimicrobial chemotherapy could eradicate H. mustelae from ferrets. Triple antimicrobial therapy combining amoxicillin, metronidazole, and bismuth subsalicylate was successful in eradicating the organism from 5 of 7 (71%) adult ferrets. Despite apparent in vitro susceptibility, neither chloramphenicol monotherapy nor a polytherapeutic regimen combining tetracycline, metronidazole, and bismuth subsalicylate proved effective in the eradication of H. mustelae. Several strains isolated after unsuccessful polytherapy showed markedly increased resistance to metronidazole. These preliminary findings are similar to results of H. pylori treatment trials with humans and suggest that the ferret may be a useful model for evaluating and comparing potential antimicrobial modalities for the eradication of H. pylori.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baskerville A., Newell D. G. Naturally occurring chronic gastritis and C pylori infection in the rhesus monkey: a potential model for gastritis in man. Gut. 1988 Apr;29(4):465–472. doi: 10.1136/gut.29.4.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borody T. J., Cole P., Noonan S., Morgan A., Lenne J., Hyland L., Brandl S., Borody E. G., George L. L. Recurrence of duodenal ulcer and Campylobacter pylori infection after eradication. Med J Aust. 1989 Oct 16;151(8):431–435. doi: 10.5694/j.1326-5377.1989.tb101251.x. [DOI] [PubMed] [Google Scholar]
  3. Bronsdon M. A., Schoenknecht F. D. Campylobacter pylori isolated from the stomach of the monkey, Macaca nemestrina. J Clin Microbiol. 1988 Sep;26(9):1725–1728. doi: 10.1128/jcm.26.9.1725-1728.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Börsch G., Mai U., Müller K. M. Monotherapy or polychemotherapy in the treatment of Campylobacter pylori-related gastroduodenal disease. Scand J Gastroenterol Suppl. 1988;142:101–106. doi: 10.3109/00365528809091722. [DOI] [PubMed] [Google Scholar]
  5. Drumm B., Sherman P., Chiasson D., Karmali M., Cutz E. Treatment of Campylobacter pylori-associated antral gastritis in children with bismuth subsalicylate and ampicillin. J Pediatr. 1988 Nov;113(5):908–912. doi: 10.1016/s0022-3476(88)80030-1. [DOI] [PubMed] [Google Scholar]
  6. Fox J. G., Cabot E. B., Taylor N. S., Laraway R. Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets. Infect Immun. 1988 Nov;56(11):2994–2996. doi: 10.1128/iai.56.11.2994-2996.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fox J. G., Edrise B. M., Cabot E. B., Beaucage C., Murphy J. C., Prostak K. S. Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res. 1986 Feb;47(2):236–239. [PubMed] [Google Scholar]
  8. Fox J. G., Lee A. Gastric campylobacter-like organisms: their role in gastric disease of laboratory animals. Lab Anim Sci. 1989 Nov;39(6):543–553. [PubMed] [Google Scholar]
  9. Fox J. G., Murphy J. C., Ackerman J. I., Prostak K. S., Gallagher C. A., Rambow V. J. Proliferative colitis in ferrets. Am J Vet Res. 1982 May;43(5):858–864. [PubMed] [Google Scholar]
  10. Fox J. G. Transmissible drug resistance in Shigella and Salmonella isolated from pet monkeys and their owners. J Med Primatol. 1975;4(3):165–171. doi: 10.1159/000459850. [DOI] [PubMed] [Google Scholar]
  11. Glupczynski Y., Burette A., Labbe M., Deprez C., De Reuck M., Deltenre M. Campylobacter pylori-associated gastritis: a double-blind placebo-controlled trial with amoxycillin. Am J Gastroenterol. 1988 Apr;83(4):365–372. [PubMed] [Google Scholar]
  12. Goodwin C. S., Armstrong J. A., Marshall B. J. Campylobacter pyloridis, gastritis, and peptic ulceration. J Clin Pathol. 1986 Apr;39(4):353–365. doi: 10.1136/jcp.39.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goodwin C. S., Marshall B. J., Blincow E. D., Wilson D. H., Blackbourn S., Phillips M. Prevention of nitroimidazole resistance in Campylobacter pylori by coadministration of colloidal bismuth subcitrate: clinical and in vitro studies. J Clin Pathol. 1988 Feb;41(2):207–210. doi: 10.1136/jcp.41.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hazell S. L., Borody T. J., Gal A., Lee A. Campylobacter pyloridis gastritis I: Detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol. 1987 Apr;82(4):292–296. [PubMed] [Google Scholar]
  15. Hirschl A. M., Hentschel E., Schütze K., Nemec H., Pötzi R., Gangl A., Weiss W., Pletschette M., Stanek G., Rotter M. L. The efficacy of antimicrobial treatment in Campylobacter pylori-associated gastritis and duodenal ulcer. Scand J Gastroenterol Suppl. 1988;142:76–81. doi: 10.3109/00365528809091718. [DOI] [PubMed] [Google Scholar]
  16. Krakowka S., Morgan D. R., Kraft W. G., Leunk R. D. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun. 1987 Nov;55(11):2789–2796. doi: 10.1128/iai.55.11.2789-2796.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Krueger K. L., Murphy J. C., Fox J. G. Treatment of proliferative colitis in ferrets. J Am Vet Med Assoc. 1989 May 15;194(10):1435–1436. [PubMed] [Google Scholar]
  18. Langenberg W., Rauws E. A., Widjojokusumo A., Tytgat G. N., Zanen H. C. Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol. 1986 Sep;24(3):414–417. doi: 10.1128/jcm.24.3.414-417.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marshall B. J., Armstrong J. A., Francis G. J., Nokes N. T., Wee S. H. Antibacterial action of bismuth in relation to Campylobacter pyloridis colonization and gastritis. Digestion. 1987;37 (Suppl 2):16–30. doi: 10.1159/000199555. [DOI] [PubMed] [Google Scholar]
  20. McNulty C. A., Dent J. C., Ford G. A., Wilkinson S. P. Inhibitory antimicrobial concentrations against Campylobacter pylori in gastric mucosa. J Antimicrob Chemother. 1988 Nov;22(5):729–738. doi: 10.1093/jac/22.5.729. [DOI] [PubMed] [Google Scholar]
  21. McNulty C. A., Gearty J. C., Crump B., Davis M., Donovan I. A., Melikian V., Lister D. M., Wise R. Campylobacter pyloridis and associated gastritis: investigator blind, placebo controlled trial of bismuth salicylate and erythromycin ethylsuccinate. Br Med J (Clin Res Ed) 1986 Sep 13;293(6548):645–649. doi: 10.1136/bmj.293.6548.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mertens J. C., Dekker W., Ligtvoet E. E., Blok P. Treatment failure of norfloxacin against Campylobacter pylori and chronic gastritis in patients with nonulcerative dyspepsia. Antimicrob Agents Chemother. 1989 Feb;33(2):256–257. doi: 10.1128/aac.33.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Morgan D., Kraft W., Bender M., Pearson A. Nitrofurans in the treatment of gastritis associated with Campylobacter pylori. The Gastrointestinal Physiology Working Group of Cayetano Heredia and The Johns Hopkins Universities. Gastroenterology. 1988 Nov;95(5):1178–1184. doi: 10.1016/0016-5085(88)90348-4. [DOI] [PubMed] [Google Scholar]
  24. Morris A., Brown P., Ali M. R., Lane M., Palmer R. Treatment of Campylobacter pylori gastritis: a pilot study using pirenzepine dihydrochloride (Gastrozepin) and three formulations of colloidal bismuth subcitrate (De-Nol). N Z Med J. 1988 Oct 26;101(856 Pt 1):651–654. [PubMed] [Google Scholar]
  25. Oderda G., Dell'Olio D., Morra I., Ansaldi N. Campylobacter pylori gastritis: long term results of treatment with amoxycillin. Arch Dis Child. 1989 Mar;64(3):326–329. doi: 10.1136/adc.64.3.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Pfeiffer C. J. Surface topology of the stomach in man and the laboratory ferret. J Ultrastruct Res. 1970 Nov;33(3):252–262. doi: 10.1016/s0022-5320(70)90020-1. [DOI] [PubMed] [Google Scholar]
  27. Sylvina T. J., Berman N. G., Fox J. G. Effects of yohimbine on bradycardia and duration of recumbency in ketamine/xylazine anesthetized ferrets. Lab Anim Sci. 1990 Mar;40(2):178–182. [PubMed] [Google Scholar]
  28. Tompkins D. S., Wyatt J. I., Rathbone B. J., West A. P. The characterization and pathological significance of gastric Campylobacter-like organisms in the ferret: a model for chronic gastritis? Epidemiol Infect. 1988 Oct;101(2):269–278. doi: 10.1017/s0950268800054182. [DOI] [PMC free article] [PubMed] [Google Scholar]

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