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. 1990 Jun;34(6):1257–1258. doi: 10.1128/aac.34.6.1257

Effects of supraphysiologic temperature and broth dilution on serum protein binding.

R L White 1, M B Kays 1, T A Armstrong 1, L V Friedrich 1
PMCID: PMC171795  PMID: 2393287

Abstract

Pooled human serum, unheated (UU) and heated at 56 degrees C for 30 (HU-30) and 60 (HU-60) min, and these media diluted 1:1 with broth (UD, HD-30, and HD-60) were used to assess the effects of supraphysiologic temperature and broth dilution on the serum protein binding of ceftriaxone. Protein binding was determined by ultrafiltration and subsequent high-performance liquid chromatography analysis. Significant differences in protein binding between UU and HU-60 (P less than 0.05), UD and HD-60 (P less than 0.01), HD-30 and HD-60 (P less than 0.01), and all undiluted and diluted media (P less than 0.01) were found. These alterations in protein binding may influence the in vitro microbiologic testing of highly protein-bound antibiotics.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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