Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1990 Jun;34(6):1278–1280. doi: 10.1128/aac.34.6.1278

Mobilization of the gentamicin resistance gene in Enterococcus faecalis.

S L Hodel-Christian 1, B E Murray 1
PMCID: PMC171802  PMID: 1975489

Abstract

Enterococcus faecalis plasmid pBEM10 (a conjugative plasmid encoding beta-lactamase production and gentamicin resistance [Gmr]) was made transfer deficient by using Tn917. Relocation of the Gmr determinant into two sites on pCF10 was observed. Restriction analysis revealed insertion of a common 2.5-kilobase-pair HindIII and a 3.9-kilobase-pair HaeIII fragment encoding Gmr, suggesting that this determinant resides on a transposon similar to Tn4001.

Full text

PDF
1278

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clewell D. B., An F. Y., White B. A., Gawron-Burke C. Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918). J Bacteriol. 1985 Jun;162(3):1212–1220. doi: 10.1128/jb.162.3.1212-1220.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Combes T., Carlier C., Courvalin P. Aminoglycoside-modifying enzyme content of a multiply resistant strain of Streptococcus faecalis. J Antimicrob Chemother. 1983 Jan;11(1):41–47. doi: 10.1093/jac/11.1.41. [DOI] [PubMed] [Google Scholar]
  3. Courvalin P., Carlier C., Collatz E. Plasmid-mediated resistance to aminocyclitol antibiotics in group D streptococci. J Bacteriol. 1980 Aug;143(2):541–551. doi: 10.1128/jb.143.2.541-551.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Currier T. C., Nester E. W. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal Biochem. 1976 Dec;76(2):431–441. doi: 10.1016/0003-2697(76)90338-9. [DOI] [PubMed] [Google Scholar]
  5. Dunny G. M., Brown B. L., Clewell D. B. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3479–3483. doi: 10.1073/pnas.75.7.3479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dunny G., Funk C., Adsit J. Direct stimulation of the transfer of antibiotic resistance by sex pheromones in Streptococcus faecalis. Plasmid. 1981 Nov;6(3):270–278. doi: 10.1016/0147-619x(81)90035-4. [DOI] [PubMed] [Google Scholar]
  7. Dunny G., Yuhasz M., Ehrenfeld E. Genetic and physiological analysis of conjugation in Streptococcus faecalis. J Bacteriol. 1982 Aug;151(2):855–859. doi: 10.1128/jb.151.2.855-859.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ehrenfeld E. E., Clewell D. B. Transfer functions of the Streptococcus faecalis plasmid pAD1: organization of plasmid DNA encoding response to sex pheromone. J Bacteriol. 1987 Aug;169(8):3473–3481. doi: 10.1128/jb.169.8.3473-3481.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferretti J. J., Gilmore K. S., Courvalin P. Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J Bacteriol. 1986 Aug;167(2):631–638. doi: 10.1128/jb.167.2.631-638.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gillespie M. T., Lyon B. R., Messerotti L. J., Skurray R. A. Chromosome- and plasmid-mediated gentamicin resistance in Staphylococcus aureus encoded by Tn4001. J Med Microbiol. 1987 Sep;24(2):139–144. doi: 10.1099/00222615-24-2-139. [DOI] [PubMed] [Google Scholar]
  11. Horodniceanu T., Bougueleret L., El-Solh N., Bieth G., Delbos F. High-level, plasmid-borne resistance to gentamicin in Streptococcus faecalis subsp. zymogenes. Antimicrob Agents Chemother. 1979 Nov;16(5):686–689. doi: 10.1128/aac.16.5.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lyon B. R., Gillespie M. T., Byrne M. E., May J. W., Skurray R. A. Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon. J Med Microbiol. 1987 Mar;23(2):101–110. doi: 10.1099/00222615-23-2-101. [DOI] [PubMed] [Google Scholar]
  14. Lyon B. R., Gillespie M. T., Skurray R. A. Detection and characterization of IS256, an insertion sequence in Staphylococcus aureus. J Gen Microbiol. 1987 Nov;133(11):3031–3038. doi: 10.1099/00221287-133-11-3031. [DOI] [PubMed] [Google Scholar]
  15. Lyon B. R., May J. W., Skurray R. A. Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Mol Gen Genet. 1984;193(3):554–556. doi: 10.1007/BF00382099. [DOI] [PubMed] [Google Scholar]
  16. Lyon B. R., Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987 Mar;51(1):88–134. doi: 10.1128/mr.51.1.88-134.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Martel A., Masson M., Moreau N., Le Goffic F. Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities. Eur J Biochem. 1983 Jul 1;133(3):515–521. doi: 10.1111/j.1432-1033.1983.tb07494.x. [DOI] [PubMed] [Google Scholar]
  18. Mederski-Samoraj B. D., Murray B. E. High-level resistance to gentamicin in clinical isolates of enterococci. J Infect Dis. 1983 Apr;147(4):751–757. doi: 10.1093/infdis/147.4.751. [DOI] [PubMed] [Google Scholar]
  19. Murray B. E., An F. Y., Clewell D. B. Plasmids and pheromone response of the beta-lactamase producer Streptococcus (Enterococcus) faecalis HH22. Antimicrob Agents Chemother. 1988 Apr;32(4):547–551. doi: 10.1128/aac.32.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murray B. E., Mederski-Samaroj B. Transferable beta-lactamase. A new mechanism for in vitro penicillin resistance in Streptococcus faecalis. J Clin Invest. 1983 Sep;72(3):1168–1171. doi: 10.1172/JCI111042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rouch D. A., Byrne M. E., Kong Y. C., Skurray R. A. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. J Gen Microbiol. 1987 Nov;133(11):3039–3052. doi: 10.1099/00221287-133-11-3039. [DOI] [PubMed] [Google Scholar]
  22. Thomas W. D., Jr, Archer G. L. Mobility of gentamicin resistance genes from staphylococci isolated in the United States: identification of Tn4031, a gentamicin resistance transposon from Staphylococcus epidermidis. Antimicrob Agents Chemother. 1989 Aug;33(8):1335–1341. doi: 10.1128/aac.33.8.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ubukata K., Yamashita N., Gotoh A., Konno M. Purification and characterization of aminoglycoside-modifying enzymes from Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 1984 Jun;25(6):754–759. doi: 10.1128/aac.25.6.754. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES