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. 2000 Feb;82(2):165–168. doi: 10.1136/adc.82.2.165

Interleukin 8 in middle ear fluid during acute otitis media: correlation with aetiology and bacterial eradication

E Leibovitz 1, R Dagan 1, J Laver 1, L Piglansky 1, S Raiz 1, M Abboud 1, D Fliss 1, A Leiberman 1, A Barzilai 1
PMCID: PMC1718196  PMID: 10648376

Abstract

OBJECTIVES—To study the concentration of interleukin 8 (IL-8) in the middle ear fluid of children with acute otitis media and the association between IL-8 concentrations, aetiology of acute otitis media, and bacteriological sterilisation.
STUDY DESIGN—Middle ear fluid was obtained by tympanocentesis at enrolment (day 1) and on day 4-5 in 81 children aged 3-36 months with acute otitis media who received antibiotic treatment. IL-8 concentrations were measured by enzyme linked immunosorbent assay.
RESULTS—101 samples were obtained on day 1 and 47 samples on day 4-5. 94 pathogens were isolated in 79 of 101 samples obtained on day 1: 56 Haemophilus influenzae, 35 Streptococcus pneumoniae, 2 Moraxella catarrhalis, and 1 Streptococcus pyogenes. Among 40paired, initially culture positive samples, sterilisation was achieved on day 4-5 in 22 but not in 18 (13 H influenzae, 2 S pneumoniae, and 3 H influenzae and S pneumoniae concomitantly). IL-8 was detected in 96 of 101 and 46 of 47 samples obtained on days 1 and 4-5, respectively. Mean (SD) IL-8 concentration on day 1 was significantly higher in culture positive than in negative samples (12 636 (23 317) v 5920 (7080) pg/ml). In paired samples, IL-8 concentration fell in 12 of 22 ears in which sterilisation was achieved and in 9 of 21 ears with persistent or new infection. Mean (SD) IL-8 concentrations on day 4-5 were significantly higher in culture positive than in negative samples (15 420 (15 418) v 6695 (5092) pg/ml).
CONCLUSIONS—Higher IL-8 concentrations are found in culture positive middle ear fluid in acute otitis media. Bacterial eradication is associated with a fall in these concentrations.



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Selected References

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  1. Baggiolini M., Walz A., Kunkel S. L. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989 Oct;84(4):1045–1049. doi: 10.1172/JCI114265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berman S. Otitis media in children. N Engl J Med. 1995 Jun 8;332(23):1560–1565. doi: 10.1056/NEJM199506083322307. [DOI] [PubMed] [Google Scholar]
  3. Bernstein J. M., Humbert J. R., Hliwa M. M. Oxygen consumption of middle ear and peripheral blood neutrophils in acute suppurative otitis media. Am J Otolaryngol. 1985 May-Jun;6(3):169–172. doi: 10.1016/s0196-0709(85)80080-6. [DOI] [PubMed] [Google Scholar]
  4. Bernstein J. M. Immunologic reactivity in the middle ear in otitis media with effusion. Otolaryngol Clin North Am. 1991 Aug;24(4):845–858. [PubMed] [Google Scholar]
  5. Carlin S. A., Marchant C. D., Shurin P. A., Johnson C. E., Super D. M., Rehmus J. M. Host factors and early therapeutic response in acute otitis media. J Pediatr. 1991 Feb;118(2):178–183. doi: 10.1016/s0022-3476(05)80479-2. [DOI] [PubMed] [Google Scholar]
  6. Chonmaitree T., Patel J. A., Sim T., Garofalo R., Uchida T., Sim T., Howie V. M., Owen M. J. Role of leukotriene B4 and interleukin-8 in acute bacterial and viral otitis media. Ann Otol Rhinol Laryngol. 1996 Dec;105(12):968–974. doi: 10.1177/000348949610501207. [DOI] [PubMed] [Google Scholar]
  7. Dagan R., Abramson O., Leibovitz E., Greenberg D., Lang R., Goshen S., Yagupsky P., Leiberman A., Fliss D. M. Bacteriologic response to oral cephalosporins: are established susceptibility breakpoints appropriate in the case of acute otitis media? J Infect Dis. 1997 Nov;176(5):1253–1259. doi: 10.1086/514120. [DOI] [PubMed] [Google Scholar]
  8. Dagan R., Leibovitz E., Greenberg D., Yagupsky P., Fliss D. M., Leiberman A. Early eradication of pathogens from middle ear fluid during antibiotic treatment of acute otitis media is associated with improved clinical outcome. Pediatr Infect Dis J. 1998 Sep;17(9):776–782. doi: 10.1097/00006454-199809000-00005. [DOI] [PubMed] [Google Scholar]
  9. Fergie J. E., Purcell K. The role of inflammatory mediators and anti-inflammatory drugs in otitis media. Pediatr Ann. 1998 Feb;27(2):76–81. doi: 10.3928/0090-4481-19980201-05. [DOI] [PubMed] [Google Scholar]
  10. Klein J. O. Otitis media. Clin Infect Dis. 1994 Nov;19(5):823–833. doi: 10.1093/clinids/19.5.823. [DOI] [PubMed] [Google Scholar]
  11. Lewis D. M., Schram J. L., Meadema S. J., Lim D. J. Experimental otitis media in chinchillas. Ann Otol Rhinol Laryngol Suppl. 1980 May-Jun;89(3 Pt 2):344–350. doi: 10.1177/00034894800890s381. [DOI] [PubMed] [Google Scholar]
  12. Maxwell K. S., Fitzgerald J. E., Burleson J. A., Leonard G., Carpenter R., Kreutzer D. L. Interleukin-8 expression in otitis media. Laryngoscope. 1994 Aug;104(8 Pt 1):989–995. doi: 10.1288/00005537-199408000-00013. [DOI] [PubMed] [Google Scholar]
  13. Miller M. D., Krangel M. S. Biology and biochemistry of the chemokines: a family of chemotactic and inflammatory cytokines. Crit Rev Immunol. 1992;12(1-2):17–46. [PubMed] [Google Scholar]
  14. Mukaida N., Harada A., Yasumoto K., Matsushima K. Properties of pro-inflammatory cell type-specific leukocyte chemotactic cytokines, interleukin 8 (IL-8) and monocyte chemotactic and activating factor (MCAF). Microbiol Immunol. 1992;36(8):773–789. doi: 10.1111/j.1348-0421.1992.tb02080.x. [DOI] [PubMed] [Google Scholar]
  15. Ovesen T., Ledet T. Bacteria and endotoxin in middle ear fluid and the course of secretory otitis media. Clin Otolaryngol Allied Sci. 1992 Dec;17(6):531–534. doi: 10.1111/j.1365-2273.1992.tb01713.x. [DOI] [PubMed] [Google Scholar]
  16. Patel J., Chonmaitree T., Schmalstieg F. Effect of modulation of polymorphonuclear leukocyte migration with anti-CD18 antibody on pathogenesis of experimental otitis media in guinea pigs. Infect Immun. 1993 Mar;61(3):1132–1135. doi: 10.1128/iai.61.3.1132-1135.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Storgaard M., Larsen K., Blegvad S., Nødgaard H., Ovesen T., Andersen P. L., Obel N. Interleukin-8 and chemotactic activity of middle ear effusions. J Infect Dis. 1997 Feb;175(2):474–477. doi: 10.1093/infdis/175.2.474. [DOI] [PubMed] [Google Scholar]
  18. Sáez-Llorens X. Pathogenesis of acute otitis media. Pediatr Infect Dis J. 1994 Nov;13(11):1035–1038. doi: 10.1097/00006454-199411000-00032. [DOI] [PubMed] [Google Scholar]
  19. Takeuchi K., Maesako K., Yuta A., Sakakura Y. Interleukin-8 gene expression in middle ear effusions. Ann Otol Rhinol Laryngol. 1994 May;103(5 Pt 1):404–407. doi: 10.1177/000348949410300511. [DOI] [PubMed] [Google Scholar]
  20. Yellon R. F., Leonard G., Marucha P. T., Craven R., Carpenter R. J., Lehmann W. B., Burleson J. A., Kreutzer D. L. Characterization of cytokines present in middle ear effusions. Laryngoscope. 1991 Feb;101(2):165–169. doi: 10.1288/00005537-199102000-00011. [DOI] [PubMed] [Google Scholar]

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