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. 2001 May;84(5):444–448. doi: 10.1136/adc.84.5.444

Uses of stable isotopes in clinical diagnosis and research in the paediatric population

O Bodamer 1, D Halliday 1
PMCID: PMC1718760  PMID: 11316697

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Selected References

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  1. Abrams S. A., Grusak M. A., Stuff J., O'Brien K. O. Calcium and magnesium balance in 9-14-y-old children. Am J Clin Nutr. 1997 Nov;66(5):1172–1177. doi: 10.1093/ajcn/66.5.1172. [DOI] [PubMed] [Google Scholar]
  2. Abrams S. A. Using stable isotopes to assess mineral absorption and utilization by children. Am J Clin Nutr. 1999 Dec;70(6):955–964. doi: 10.1093/ajcn/70.6.955. [DOI] [PubMed] [Google Scholar]
  3. Bandini L. G., Schoeller D. A., Cyr H. N., Dietz W. H. Validity of reported energy intake in obese and nonobese adolescents. Am J Clin Nutr. 1990 Sep;52(3):421–425. doi: 10.1093/ajcn/52.3.421. [DOI] [PubMed] [Google Scholar]
  4. Berry G. T., Nissim I., Gibson J. B., Mazur A. T., Lin Z., Elsas L. J., Singh R. H., Klein P. D., Segal S. Quantitative assessment of whole body galactose metabolism in galactosemic patients. Eur J Pediatr. 1997 Aug;156 (Suppl 1):S43–S49. doi: 10.1007/pl00014271. [DOI] [PubMed] [Google Scholar]
  5. Bier D. M. Stable isotopes in biosciences, their measurement and models for amino acid metabolism. Eur J Pediatr. 1997 Aug;156 (Suppl 1):S2–S8. doi: 10.1007/pl00014265. [DOI] [PubMed] [Google Scholar]
  6. Bodamer O. A., Leonard J. V., Tasker R. C., Hoffmann G. F., Halliday D. Protein turnover in critically ill children. Eur J Pediatr. 1997 Aug;156 (Suppl 1):S59–S61. doi: 10.1007/pl00014273. [DOI] [PubMed] [Google Scholar]
  7. Bodamer O. A., Mayatepek E., Leonard J. V. Leucine and glucose kinetics in glycogen storage disease type IIIa. J Inherit Metab Dis. 1997 Nov;20(6):847–847. doi: 10.1023/a:1005304809354. [DOI] [PubMed] [Google Scholar]
  8. Bougnères P. F., Karl I. E., Hillman L. S., Bier D. M. Lipid transport in the human newborn. Palmitate and glycerol turnover and the contribution of glycerol to neonatal hepatic glucose output. J Clin Invest. 1982 Aug;70(2):262–270. doi: 10.1172/JCI110613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bougnères P. F., Rocchiccioli F., Nurjhan N., Zeller J. Stable isotope determination of plasma lactate conversion into glucose in fasting infants. Am J Physiol. 1995 Apr;268(4 Pt 1):E652–E659. doi: 10.1152/ajpendo.1995.268.4.E652. [DOI] [PubMed] [Google Scholar]
  10. Bradbury M. G., Brocklebank J. T., Smye S. W., Davies P. S. Total body water measurement in renal insufficiency. Pediatr Nephrol. 1996 Apr;10(2):195–199. doi: 10.1007/BF00862075. [DOI] [PubMed] [Google Scholar]
  11. Bronner F., Abrams S. A. Development and regulation of calcium metabolism in healthy girls. J Nutr. 1998 Sep;128(9):1474–1480. doi: 10.1093/jn/128.9.1474. [DOI] [PubMed] [Google Scholar]
  12. Calder A. G., Anderson S. E., Grant I., McNurlan M. A., Garlick P. J. The determination of low d5-phenylalanine enrichment (0.002-0.09 atom percent excess), after conversion to phenylethylamine, in relation to protein turnover studies by gas chromatography/electron ionization mass spectrometry. Rapid Commun Mass Spectrom. 1992 Jul;6(7):421–424. doi: 10.1002/rcm.1290060704. [DOI] [PubMed] [Google Scholar]
  13. Carnielli V. P., Wattimena D. J., Luijendijk I. H., Boerlage A., Degenhart H. J., Sauer P. J. The very low birth weight premature infant is capable of synthesizing arachidonic and docosahexaenoic acids from linoleic and linolenic acids. Pediatr Res. 1996 Jul;40(1):169–174. doi: 10.1203/00006450-199607000-00029. [DOI] [PubMed] [Google Scholar]
  14. Castillo L., Yu Y. M., Marchini J. S., Chapman T. E., Sanchez M., Young V. R., Burke J. F. Phenylalanine and tyrosine kinetics in critically ill children with sepsis. Pediatr Res. 1994 May;35(5):580–588. [PubMed] [Google Scholar]
  15. Clark S. E., Karn C. A., Ahlrichs J. A., Wang J., Leitch C. A., Leichty E. A., Denne S. C. Acute changes in leucine and phenylalanine kinetics produced by parenteral nutrition in premature infants. Pediatr Res. 1997 Apr;41(4 Pt 1):568–574. doi: 10.1203/00006450-199704000-00019. [DOI] [PubMed] [Google Scholar]
  16. Cogo P. E., Giordano G., Badon T., Orzali A., Zimmermann I. U., Zacchello F., Sauer P. J., Carnielli V. P. Simultaneous measurement of the rates of appearance of palmitic and linoleic acid in critically ill infants. Pediatr Res. 1997 Feb;41(2):178–182. doi: 10.1203/00006450-199702000-00004. [DOI] [PubMed] [Google Scholar]
  17. Crane C. W., Jenner F. A., Pollitt R. J. The relationship between the formation of urea and argininosuccinate in a patient with argininosuccinic aciduria studied with labelled precursors. Clin Sci Mol Med. 1974 Jun;46(6):715–727. doi: 10.1042/cs0460715. [DOI] [PubMed] [Google Scholar]
  18. Crews H. M., Ducros V., Eagles J., Mellon F. A., Kastenmayer P., Luten J. B., McGaw B. A. Mass spectrometric methods for studying nutrient mineral and trace element absorption and metabolism in humans using stable isotopes. A review. Analyst. 1994 Nov;119(11):2491–2514. doi: 10.1039/an9941902491. [DOI] [PubMed] [Google Scholar]
  19. Daley S. E., Pearson A. D., Craft A. W., Kernahan J., Wyllie R. A., Price L., Brock C., Hetherington C., Halliday D., Bartlett K. Whole body protein metabolism in children with cancer. Arch Dis Child. 1996 Oct;75(4):273–281. doi: 10.1136/adc.75.4.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Davidsson L., Galan P., Cherouvrier F., Kastenmayer P., Juillerat M. A., Hercberg S., Hurrell R. F. Bioavailability in infants of iron from infant cereals: effect of dephytinization. Am J Clin Nutr. 1997 Apr;65(4):916–920. doi: 10.1093/ajcn/65.4.916. [DOI] [PubMed] [Google Scholar]
  21. Davies P. S., Clough H., Bishop N. J., Lucas A., Cole J. J., Cole T. J. Total energy expenditure in small for gestational age infants. Arch Dis Child Fetal Neonatal Ed. 1996 Jul;75(1):F46–F48. doi: 10.1136/fn.75.1.f46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Davies P. S. Energy requirements for growth and development in infancy. Am J Clin Nutr. 1998 Oct;68(4):939S–943S. doi: 10.1093/ajcn/68.4.939S. [DOI] [PubMed] [Google Scholar]
  23. Ellerine N. P., Herring W. J., Elsas L. J., 2nd, McKean M. C., Klein P. D., Danner D. J. Thiamin-responsive maple syrup urine disease in a patient antigenically missing dihydrolipoamide acyltransferase. Biochem Med Metab Biol. 1993 Jun;49(3):363–374. doi: 10.1006/bmmb.1993.1037. [DOI] [PubMed] [Google Scholar]
  24. Fjeld C. R., Freundt-Thurne J., Schoeller D. A. Total body water measured by 18-O dilution and bioelectrical impedance in well and malnourished children. Pediatr Res. 1990 Jan;27(1):98–102. doi: 10.1203/00006450-199001000-00024. [DOI] [PubMed] [Google Scholar]
  25. Fjeld C. R., Schoeller D. A. Energy expenditure of malnourished children during catch-up growth. Proc Nutr Soc. 1988 Sep;47(3):227–231. doi: 10.1079/pns19880039. [DOI] [PubMed] [Google Scholar]
  26. Fox T. E., Fairweather-Tait S. J., Eagles J., Wharf S. G. Intrinsic labelling of different foods with stable isotope of zinc (67Zn) for use in bioavailability studies. Br J Nutr. 1991 Jul;66(1):57–63. doi: 10.1079/bjn19910009. [DOI] [PubMed] [Google Scholar]
  27. Hambidge K. M., Krebs N. F., Miller L. Evaluation of zinc metabolism with use of stable-isotope techniques: implications for the assessment of zinc status. Am J Clin Nutr. 1998 Aug;68(2 Suppl):410S–413S. doi: 10.1093/ajcn/68.2.410S. [DOI] [PubMed] [Google Scholar]
  28. Jones P. J., Leatherdale S. T. Stable isotopes in clinical research: safety reaffirmed. Clin Sci (Lond) 1991 Apr;80(4):277–280. doi: 10.1042/cs0800277. [DOI] [PubMed] [Google Scholar]
  29. Kalderon B., Korman S. H., Gutman A., Lapidot A. Glucose recycling and production in glycogenosis type I and III: stable isotope technique study. Am J Physiol. 1989 Sep;257(3 Pt 1):E346–E353. doi: 10.1152/ajpendo.1989.257.3.E346. [DOI] [PubMed] [Google Scholar]
  30. Kalhan S. C. Stable isotope tracers for studies of glucose metabolism. J Lab Clin Med. 1990 Nov;116(5):615–622. [PubMed] [Google Scholar]
  31. Kalhan S., Parimi P. Gluconeogenesis in the fetus and neonate. Semin Perinatol. 2000 Apr;24(2):94–106. doi: 10.1053/sp.2000.6360. [DOI] [PubMed] [Google Scholar]
  32. Kandil H., Darwish O., Hammad S., Zagloul N., Halliday D., Millward J. Nitrogen balance and protein turnover during the growth failure in newly born low-birth-weight infants. Am J Clin Nutr. 1991 Jun;53(6):1411–1417. doi: 10.1093/ajcn/53.6.1411. [DOI] [PubMed] [Google Scholar]
  33. Kien C. L., Rohrbaugh D. K., Burke J. F., Young V. R. Whole body protein synthesis in relation to basal energy expenditure in healthy children and in children recovering from burn injury. Pediatr Res. 1978 Mar;12(3):211–216. doi: 10.1203/00006450-197803000-00010. [DOI] [PubMed] [Google Scholar]
  34. Kien C. L., Zipf W. B., Horswill C. A., Denne S. C., McCoy K. S., O'Dorisio T. M. Effects of feeding on protein turnover in healthy children and in children with cystic fibrosis. Am J Clin Nutr. 1996 Oct;64(4):608–614. doi: 10.1093/ajcn/64.4.608. [DOI] [PubMed] [Google Scholar]
  35. Kossak B. D., Schmidt-Sommerfeld E., Schoeller D. A., Rinaldo P., Penn D., Tonsgard J. H. Impaired fatty acid oxidation in children on valproic acid and the effect of L-carnitine. Neurology. 1993 Nov;43(11):2362–2368. doi: 10.1212/wnl.43.11.2362. [DOI] [PubMed] [Google Scholar]
  36. Lapidot A. Inherited disorders of carbohydrate metabolism in children studied by 13C-labelled precursors, NMR and GC-MS. J Inherit Metab Dis. 1990;13(4):466–475. doi: 10.1007/BF01799504. [DOI] [PubMed] [Google Scholar]
  37. Lee B., Yu H., Jahoor F., O'Brien W., Beaudet A. L., Reeds P. In vivo urea cycle flux distinguishes and correlates with phenotypic severity in disorders of the urea cycle. Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8021–8026. doi: 10.1073/pnas.140082197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Maffeis C., Armellini F., Tatò L., Schutz Y. Fat oxidation and adiposity in prepubertal children: exogenous versus endogenous fat utilization. J Clin Endocrinol Metab. 1999 Feb;84(2):654–658. doi: 10.1210/jcem.84.2.5463. [DOI] [PubMed] [Google Scholar]
  39. Mitton S. G., Calder A. G., Garlick P. J. Protein turnover rates in sick, premature neonates during the first few days of life. Pediatr Res. 1991 Nov;30(5):418–422. doi: 10.1203/00006450-199111000-00005. [DOI] [PubMed] [Google Scholar]
  40. Mitton S. G., Garlick P. J. Changes in protein turnover after the introduction of parenteral nutrition in premature infants: comparison of breast milk and egg protein-based amino acid solutions. Pediatr Res. 1992 Oct;32(4):447–454. doi: 10.1203/00006450-199210000-00015. [DOI] [PubMed] [Google Scholar]
  41. Morlese J. F., Forrester T., Badaloo A., Del Rosario M., Frazer M., Jahoor F. Albumin kinetics in edematous and nonedematous protein-energy malnourished children. Am J Clin Nutr. 1996 Dec;64(6):952–959. doi: 10.1093/ajcn/64.6.952. [DOI] [PubMed] [Google Scholar]
  42. Nicholson J. F., Freeman J. M. Metabolism of compounds labeled with 15 N by an infant with congenital hyperammonemia. Pediatr Res. 1972 Apr;6(4):252–260. doi: 10.1203/00006450-197204000-00007. [DOI] [PubMed] [Google Scholar]
  43. Pacy P. J., Cheng K. N., Thompson G. N., Halliday D. Stable isotopes as tracers in clinical research. Ann Nutr Metab. 1989;33(2):65–78. doi: 10.1159/000177523. [DOI] [PubMed] [Google Scholar]
  44. Patterson B. W., Zhao G., Klein S. Improved accuracy and precision of gas chromatography/mass spectrometry measurements for metabolic tracers. Metabolism. 1998 Jun;47(6):706–712. doi: 10.1016/s0026-0495(98)90035-x. [DOI] [PubMed] [Google Scholar]
  45. Pons G., Blais J. C., Rey E., Plissonnier M., Richard M. O., Carrier O., d'Athis P., Moran C., Badoual J., Olive G. Maturation of caffeine N-demethylation in infancy: a study using the 13CO2 breath test. Pediatr Res. 1988 Jun;23(6):632–636. doi: 10.1203/00006450-198806000-00021. [DOI] [PubMed] [Google Scholar]
  46. Powis M. R., Smith K., Rennie M., Halliday D., Pierro A. Effect of major abdominal operations on energy and protein metabolism in infants and children. J Pediatr Surg. 1998 Jan;33(1):49–53. doi: 10.1016/s0022-3468(98)90360-8. [DOI] [PubMed] [Google Scholar]
  47. Rivera A., Jr, Bell E. F., Bier D. M. Effect of intravenous amino acids on protein metabolism of preterm infants during the first three days of life. Pediatr Res. 1993 Feb;33(2):106–111. doi: 10.1203/00006450-199302000-00003. [DOI] [PubMed] [Google Scholar]
  48. Roberts S. B., Coward W. A., Schlingenseipen K. H., Nohria V., Lucas A. Comparison of the doubly labeled water (2H2(18)O) method with indirect calorimetry and a nutrient-balance study for simultaneous determination of energy expenditure, water intake, and metabolizable energy intake in preterm infants. Am J Clin Nutr. 1986 Sep;44(3):315–322. doi: 10.1093/ajcn/44.3.315. [DOI] [PubMed] [Google Scholar]
  49. Rowland M., Lambert I., Gormally S., Daly L. E., Thomas J. E., Hetherington C., Durnin M., Drumm B. Carbon 13-labeled urea breath test for the diagnosis of Helicobacter pylori infection in children. J Pediatr. 1997 Dec;131(6):815–820. doi: 10.1016/s0022-3476(97)70026-x. [DOI] [PubMed] [Google Scholar]
  50. Schadewaldt P., Bodner A., Brösicke H., Hammen H. W., Wendel U. Assessment of whole body L-leucine oxidation by noninvasive L-[1-13C]leucine breath tests: a reappraisal in patients with maple syrup urine disease, obligate heterozygotes, and healthy subjects. Pediatr Res. 1998 May;43(5):592–600. doi: 10.1203/00006450-199805000-00006. [DOI] [PubMed] [Google Scholar]
  51. Schneider J. F., Schoeller D. A., Nemchausky B., Boyer J. L., Klein P. Validation of 13CO2 breath analysis as a measurement of demethylation of stable isotope labeled aminopyrine in man. Clin Chim Acta. 1978 Mar 1;84(1-2):153–162. doi: 10.1016/0009-8981(78)90489-8. [DOI] [PubMed] [Google Scholar]
  52. Schoeller D. A., van Santen E. Measurement of energy expenditure in humans by doubly labeled water method. J Appl Physiol Respir Environ Exerc Physiol. 1982 Oct;53(4):955–959. doi: 10.1152/jappl.1982.53.4.955. [DOI] [PubMed] [Google Scholar]
  53. Serfass R. E., Ziegler E. E., Edwards B. B., Houk R. S. Intrinsic and extrinsic stable isotopic zinc absorption by infants from formulas. J Nutr. 1989 Nov;119(11):1661–1669. doi: 10.1093/jn/119.11.1661. [DOI] [PubMed] [Google Scholar]
  54. Shortland G. J., Walter J. H., Fleming P. J., Halliday D. Phenylalanine kinetics in sick preterm neonates with respiratory distress syndrome. Pediatr Res. 1994 Dec;36(6):713–718. doi: 10.1203/00006450-199412000-00005. [DOI] [PubMed] [Google Scholar]
  55. Stettler N., Kawachak D. A., Boyle L. L., Propert K. J., Scanlin T. F., Stallings V. A., Zemel B. S. A prospective study of body composition changes in children with cystic fibrosis. Ann N Y Acad Sci. 2000 May;904:406–409. doi: 10.1111/j.1749-6632.2000.tb06490.x. [DOI] [PubMed] [Google Scholar]
  56. Suprasongsin C., Kalhan S., Arslanian S. Determination of body composition in children and adolescents: validation of bioelectrical impedance with isotope dilution technique. J Pediatr Endocrinol Metab. 1995 Apr-Jun;8(2):103–109. doi: 10.1515/jpem.1995.8.2.103. [DOI] [PubMed] [Google Scholar]
  57. Thompson G. N., Francis D. E., Halliday D. Acute illness in maple syrup urine disease: dynamics of protein metabolism and implications for management. J Pediatr. 1991 Jul;119(1 Pt 1):35–41. doi: 10.1016/s0022-3476(05)81035-2. [DOI] [PubMed] [Google Scholar]
  58. Thompson G. N., Halliday D. Significant phenylalanine hydroxylation in vivo in patients with classical phenylketonuria. J Clin Invest. 1990 Jul;86(1):317–322. doi: 10.1172/JCI114701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  59. Thompson G. N., Pacy P. J., Ford G. C., Halliday D. Practical considerations in the use of stable isotope labelled compounds as tracers in clinical studies. Biomed Environ Mass Spectrom. 1989 May;18(5):321–327. doi: 10.1002/bms.1200180507. [DOI] [PubMed] [Google Scholar]
  60. Thompson G. N., Walter J. H., Leonard J. V., Halliday D. In vivo enzyme activity in inborn errors of metabolism. Metabolism. 1990 Aug;39(8):799–807. doi: 10.1016/0026-0495(90)90122-s. [DOI] [PubMed] [Google Scholar]
  61. Tyrala E. E., Chen X., Boden G. Glucose metabolism in the infant weighing less than 1100 grams. J Pediatr. 1994 Aug;125(2):283–287. doi: 10.1016/s0022-3476(94)70212-8. [DOI] [PubMed] [Google Scholar]
  62. Van Goudoever J. B., Colen T., Wattimena J. L., Huijmans J. G., Carnielli V. P., Sauer P. J. Immediate commencement of amino acid supplementation in preterm infants: effect on serum amino acid concentrations and protein kinetics on the first day of life. J Pediatr. 1995 Sep;127(3):458–465. doi: 10.1016/s0022-3476(95)70083-8. [DOI] [PubMed] [Google Scholar]
  63. Van Goudoever J. B., Sulkers E. J., Halliday D., Degenhart H. J., Carnielli V. P., Wattimena J. L., Sauer P. J. Whole-body protein turnover in preterm appropriate for gestational age and small for gestational age infants: comparison of [15N]glycine and [1-(13)C]leucine administered simultaneously. Pediatr Res. 1995 Apr;37(4 Pt 1):381–388. doi: 10.1203/00006450-199504000-00001. [DOI] [PubMed] [Google Scholar]
  64. Weaver C. M., Heaney R. P., Martin B. R., Fitzsimmons M. L. Extrinsic vs intrinsic labeling of the calcium in whole-wheat flour. Am J Clin Nutr. 1992 Feb;55(2):452–454. doi: 10.1093/ajcn/55.2.452. [DOI] [PubMed] [Google Scholar]
  65. Weaver L. T. Stable isotope breath tests. Nutrition. 1998 Oct;14(10):826–829. doi: 10.1016/s0899-9007(98)00094-x. [DOI] [PubMed] [Google Scholar]
  66. Wren R. E., Blume H., Mazariegos M., Solomons N., Alvarez J. O., Goran M. I. Body composition, resting metabolic rate, and energy requirements of short- and normal-stature, low-income Guatemalan children. Am J Clin Nutr. 1997 Aug;66(2):406–412. doi: 10.1093/ajcn/66.2.406. [DOI] [PubMed] [Google Scholar]
  67. Yu Y. M., Sheridan R. L., Burke J. F., Chapman T. E., Tompkins R. G., Young V. R. Kinetics of plasma arginine and leucine in pediatric burn patients. Am J Clin Nutr. 1996 Jul;64(1):60–66. doi: 10.1093/ajcn/64.1.60. [DOI] [PubMed] [Google Scholar]
  68. Yudkoff M., Daikhin Y., Nissim I., Jawad A., Wilson J., Batshaw M. In vivo nitrogen metabolism in ornithine transcarbamylase deficiency. J Clin Invest. 1996 Nov 1;98(9):2167–2173. doi: 10.1172/JCI119023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. Yudkoff M., Nissim I., McNellis W., Polin R. Albumin synthesis in premature infants: determination of turnover with [15N]glycine. Pediatr Res. 1987 Jan;21(1):49–53. doi: 10.1203/00006450-198701000-00012. [DOI] [PubMed] [Google Scholar]
  70. de Meer K., Gulmans V. A., Westerterp K. R., Houwen R. H., Berger R. Skinfold measurements in children with cystic fibrosis: monitoring fat-free mass and exercise effects. Eur J Pediatr. 1999 Oct;158(10):800–806. doi: 10.1007/s004310051209. [DOI] [PubMed] [Google Scholar]
  71. van Lingen R. A., van Goudoever J. B., Luijendijk I. H., Wattimena J. L., Sauer P. J. Effects of early amino acid administration during total parenteral nutrition on protein metabolism in pre-term infants. Clin Sci (Lond) 1992 Feb;82(2):199–203. doi: 10.1042/cs0820199. [DOI] [PubMed] [Google Scholar]
  72. van Spronsen F. J., Reijngoud D. J., Smit G. P., Nagel G. T., Stellaard F., Berger R., Heymans H. S. Phenylketonuria. The in vivo hydroxylation rate of phenylalanine into tyrosine is decreased. J Clin Invest. 1998 Jun 15;101(12):2875–2880. doi: 10.1172/JCI737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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