Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1990 Sep;34(9):1629–1632. doi: 10.1128/aac.34.9.1629

Effect of rifabutin on disseminated Mycobacterium avium infections in thymectomized, CD4 T-cell-deficient mice.

S K Furney 1, A D Roberts 1, I M Orme 1
PMCID: PMC171895  PMID: 2178333

Abstract

Disseminated Mycobacterium avium infection is the major cause of bacteremia in patients with acquired immunodeficiency syndrome. We present here a new animal model of this disease, thymectomized C57BL/6 mice that were intravenously infused with monoclonal antibody to selectively deplete CD4+ T cells. The increased susceptibility of such animals to M. avium infection is comparable to that of C57BL/6 beige mice and thus may provide a viable alternative to the latter model. Further, using representative strains of acquired immunodeficiency syndrome-associated M. avium (serotypes 1, 4, and 8 and a rough isolate), we show that the course of such infections in thymectomized, CD4-deficient mice can be markedly restrained and in some cases the infections can be sterilized by treatment over a 120-day period with a regimen containing 40 mg of the new antimycobacterial agent rifabutin per kg (body weight).

Full text

PDF
1629

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaisson R. E., Hopewell P. C. Mycobacteria and AIDS mortality. Am Rev Respir Dis. 1989 Jan;139(1):1–3. doi: 10.1164/ajrccm/139.1.1. [DOI] [PubMed] [Google Scholar]
  2. Cheers C., Waller R. Activated macrophages in congenitally athymic "nude mice" and in lethally irradiate mice. J Immunol. 1975 Sep;115(3):844–847. [PubMed] [Google Scholar]
  3. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29–56. doi: 10.1111/j.1600-065x.1983.tb01083.x. [DOI] [PubMed] [Google Scholar]
  4. Emmerling P., Finger H., Bockemühl J. Listeria monocytogenes infection in nude mice. Infect Immun. 1975 Aug;12(2):437–439. doi: 10.1128/iai.12.2.437-439.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gangadharam P. R., Edwards C. K., 3rd, Murthy P. S., Pratt P. F. An acute infection model for Mycobacterium intracellulare disease using beige mice: preliminary results. Am Rev Respir Dis. 1983 May;127(5):648–649. doi: 10.1164/arrd.1983.127.5.648. [DOI] [PubMed] [Google Scholar]
  6. Gangadharam P. R., Perumal V. K., Podapati N. R., Kesavalu L., Iseman M. D. In vivo activity of amikacin alone or in combination with clofazimine or rifabutin or both against acute experimental Mycobacterium avium complex infections in beige mice. Antimicrob Agents Chemother. 1988 Sep;32(9):1400–1403. doi: 10.1128/aac.32.9.1400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heifets L. B. Synergistic effect of rifampin, streptomycin, ethionamide, and ethambutol on Mycobacterium intracellulare. Am Rev Respir Dis. 1982 Jan;125(1):43–48. doi: 10.1164/arrd.1982.125.1.43. [DOI] [PubMed] [Google Scholar]
  8. Orme I. M. Antimycobacterial activity in vivo of LM427 (rifabutin). Am Rev Respir Dis. 1988 Nov;138(5):1254–1257. doi: 10.1164/ajrccm/138.5.1254. [DOI] [PubMed] [Google Scholar]
  9. Ozenne G., Morel A., Menard J. F., Thauvin C., Samain J. P., Lemeland J. F. Susceptibility of Mycobacterium avium complex to various two-drug combinations of antituberculosis agents. Am Rev Respir Dis. 1988 Oct;138(4):878–881. doi: 10.1164/ajrccm/138.4.878. [DOI] [PubMed] [Google Scholar]
  10. Roder J. C., Lohmann-Matthes M. L., Domzig W., Wigzell H. The beige mutation in the mouse. II. Selectivity of the natural killer (NK) cell defect. J Immunol. 1979 Nov;123(5):2174–2181. [PubMed] [Google Scholar]
  11. Roder J. C. The beige mutation in the mouse. I. A stem cell predetermined impairment in natural killer cell function. J Immunol. 1979 Nov;123(5):2168–2173. [PubMed] [Google Scholar]
  12. Roder J., Duwe A. The beige mutation in the mouse selectively impairs natural killer cell function. Nature. 1979 Mar 29;278(5703):451–453. doi: 10.1038/278451a0. [DOI] [PubMed] [Google Scholar]
  13. Yajko D. M., Kirihara J., Sanders C., Nassos P., Hadley W. K. Antimicrobial synergism against Mycobacterium avium complex strains isolated from patients with acquired immune deficiency syndrome. Antimicrob Agents Chemother. 1988 Sep;32(9):1392–1395. doi: 10.1128/aac.32.9.1392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yanagihara D. L., Barr V. L., Knisley C. V., Tsang A. Y., McClatchy J. K., Brennan P. J. Enzyme-linked immunosorbent assay of glycolipid antigens for identification of mycobacteria. J Clin Microbiol. 1985 Apr;21(4):569–574. doi: 10.1128/jcm.21.4.569-574.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Young L. S. Mycobacterium avium complex infection. J Infect Dis. 1988 May;157(5):863–867. doi: 10.1093/infdis/157.5.863. [DOI] [PubMed] [Google Scholar]
  16. Zimmer B. L., DeYoung D. R., Roberts G. D. In vitro synergistic activity of ethambutol, isoniazid, kanamycin, rifampin, and streptomycin against Mycobacterium avium-intracellulare complex. Antimicrob Agents Chemother. 1982 Jul;22(1):148–150. doi: 10.1128/aac.22.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES