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. 1990 Sep;34(9):1733–1738. doi: 10.1128/aac.34.9.1733

Comparative postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against intracellular multiplication of Legionella pneumophila serogroup 1.

P Rajagopalan-Levasseur 1, E Dournon 1, G Dameron 1, J L Vilde 1, J J Pocidalo 1
PMCID: PMC171914  PMID: 2285286

Abstract

The inhibitory and postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against virulent Legionella pneumophila serogroup 1 were evaluated in cell-free and cellular models. In the absence of macrophages (with the tissue culture medium alone), bacterial numbers remained unchanged at 24 h in the presence of 0.1 microgram of pefloxacin, ciprofloxacin, or ofloxacin per ml and 1.0 microgram of pefloxacin per ml, whereas they were reduced in the presence of 1.0 microgram of ciprofloxacin or ofloxacin per ml. Experiments to evaluate the postantibacterial effects of these drugs were therefore performed with concentrations of 0.1 microgram/ml. In the cell-free model, brief exposure (1 h) of bacteria to each antimicrobial agent resulted in a transient decrease in numbers followed by logarithmic growth. In the cellular model, all three drugs (at 0.1 and 1.0 microgram/ml) inhibited the intracellular multiplication of L. pneumophila. The intracellular postantibacterial effects of 0.1 microgram of pefloxacin, ciprofloxacin, and ofloxacin per ml, which were left in contact with L. pneumophila-infected human macrophages for 24 h, were evaluated at various times after removal of the drugs. Pefloxacin was found to exhibit a significant inhibitory effect at 72 h, whereas following the removal of ciprofloxacin and ofloxacin, rapid bacterial multiplication occurred, leading to the destruction of the macrophage monolayer within 48 h. Thus, while pefloxacin, ciprofloxacin, and ofloxacin all inhibited the multiplication of L. pneumophila in human monocyte-derived macrophages, only pefloxacin exhibited a prolonged postantibacterial effect.

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Selected References

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  1. Boogaerts M. A., Malbrain S., Scheers W., Verwilghen R. L. Effects of quinolones on granulocyte function in vitro. Infection. 1986;14 (Suppl 4):S258–S262. doi: 10.1007/BF01661288. [DOI] [PubMed] [Google Scholar]
  2. Delfino D., Bonina L., Berlinghieri M. C., Mastroeni P. Effects of a new quinoline derivative, ciprofloxacin, on some professional phagocytic cell functions. Chemioterapia. 1985 Dec;4(6):463–466. [PubMed] [Google Scholar]
  3. Desnottes J. F., Jacotot F., Bruel J., Bassoullet M. T., Niel G. Effects of pefloxacin on phagocytosis function of rat macrophages and polymorphonuclear leucocytes. J Antimicrob Chemother. 1986 Apr;17 (Suppl B):53–57. doi: 10.1093/jac/17.suppl_b.53. [DOI] [PubMed] [Google Scholar]
  4. Desnottes J. F. Quinolones et phagocytes. Pathol Biol (Paris) 1987 Dec;35(10 Pt 2):1426–1430. [PubMed] [Google Scholar]
  5. Dournon E., Rajagopalan P., Vilde J. L., Pocidalo J. J. Efficacy of pefloxacin in comparison with erythromycin in the treatment of experimental guinea pig legionellosis. J Antimicrob Chemother. 1986 Apr;17 (Suppl B):41–48. doi: 10.1093/jac/17.suppl_b.41. [DOI] [PubMed] [Google Scholar]
  6. Easmon C. S., Crane J. P., Blowers A. Effect of ciprofloxacin on intracellular organisms: in-vitro and in-vivo studies. J Antimicrob Chemother. 1986 Nov;18 (Suppl 500):43–48. doi: 10.1093/jac/18.supplement_d.43. [DOI] [PubMed] [Google Scholar]
  7. Easmon C. S., Crane J. P. Uptake of ciprofloxacin by human neutrophils. J Antimicrob Chemother. 1985 Jul;16(1):67–73. doi: 10.1093/jac/16.1.67. [DOI] [PubMed] [Google Scholar]
  8. Easmon C. S., Crane J. P. Uptake of ciprofloxacin by macrophages. J Clin Pathol. 1985 Apr;38(4):442–444. doi: 10.1136/jcp.38.4.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Edelstein P. H., Calarco K., Yasui V. K. Antimicrobial therapy of experimentally induced Legionnaires' disease in guinea pigs. Am Rev Respir Dis. 1984 Nov;130(5):849–856. doi: 10.1164/arrd.1984.130.5.849. [DOI] [PubMed] [Google Scholar]
  10. Edelstein P. H., Edelstein M. A. WIN 57273 is bactericidal for Legionella pneumophila grown in alveolar macrophages. Antimicrob Agents Chemother. 1989 Dec;33(12):2132–2136. doi: 10.1128/aac.33.12.2132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Edelstein P. H., Meyer R. D. Susceptibility of Legionella pneumophila to twenty antimicrobial agents. Antimicrob Agents Chemother. 1980 Sep;18(3):403–408. doi: 10.1128/aac.18.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fantoni M., Tamburrini E., Pallavicini F., Antinori A., Nervo P. Influence of ofloxacin and pefloxacin on human lymphocyte immunoglobulin secretion and on polymorphonuclear leucocyte superoxide anion production. J Antimicrob Chemother. 1988 Aug;22(2):193–196. doi: 10.1093/jac/22.2.193. [DOI] [PubMed] [Google Scholar]
  13. Fitzgeorge R. B., Baskerville A., Featherstone A. S. Treatment of experimental Legionnaires' disease by aerosol administration of rifampicin, ciprofloxacin, and erythromycin. Lancet. 1986 Mar 1;1(8479):502–503. doi: 10.1016/s0140-6736(86)92960-0. [DOI] [PubMed] [Google Scholar]
  14. Fitzgeorge R. B., Gibson D. H., Jepras R., Baskerville A. Studies on ciprofloxacin therapy of experimental Legionnaires' disease. J Infect. 1985 May;10(3):194–203. doi: 10.1016/s0163-4453(85)92438-7. [DOI] [PubMed] [Google Scholar]
  15. Fitzgeorge R. B. The effect of antibiotics on the growth of Legionella pneumophila in guinea-pig alveolar phagocytes infected in vivo by an aerosol. J Infect. 1985 May;10(3):189–193. doi: 10.1016/s0163-4453(85)92413-2. [DOI] [PubMed] [Google Scholar]
  16. Forsgren A., Bergkvist P. I. Effect of ciprofloxacin on phagocytosis. Eur J Clin Microbiol. 1985 Dec;4(6):575–578. doi: 10.1007/BF02013398. [DOI] [PubMed] [Google Scholar]
  17. Hand W. L., King-Thompson N. L., Steinberg T. H. Interactions of antibiotics and phagocytes. J Antimicrob Chemother. 1983 Oct;12 (Suppl 100):1–11. doi: 10.1093/jac/12.suppl_c.1. [DOI] [PubMed] [Google Scholar]
  18. Havlichek D., Pohlod D., Saravolatz L. Comparison of ciprofloxacin and rifampicin in experimental Legionella pneumophila pneumonia. J Antimicrob Chemother. 1987 Dec;20(6):875–881. doi: 10.1093/jac/20.6.875. [DOI] [PubMed] [Google Scholar]
  19. Havlichek D., Saravolatz L., Pohlod D. Effect of quinolones and other antimicrobial agents on cell-associated Legionella pneumophila. Antimicrob Agents Chemother. 1987 Oct;31(10):1529–1534. doi: 10.1128/aac.31.10.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Horwitz M. A., Silverstein S. C. Intracellular multiplication of Legionnaires' disease bacteria (Legionella pneumophila) in human monocytes is reversibly inhibited by erythromycin and rifampin. J Clin Invest. 1983 Jan;71(1):15–26. doi: 10.1172/JCI110744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Horwitz M. A., Silverstein S. C. Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes. J Clin Invest. 1980 Sep;66(3):441–450. doi: 10.1172/JCI109874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kohno S., Yamaguchi K., Dohtsu Y., Koga H., Hayashi T., Hirota M., Saito A., Hara K. Efficacy of NY-198 against experimental Legionnaires disease. Antimicrob Agents Chemother. 1988 Sep;32(9):1427–1429. doi: 10.1128/aac.32.9.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Milatovic D. Intraphagocytic activity of ciprofloxacin and CI 934. Eur J Clin Microbiol. 1986 Dec;5(6):659–660. doi: 10.1007/BF02013293. [DOI] [PubMed] [Google Scholar]
  24. Miller M. F., Martin J. R., Johnson P., Ulrich J. T., Rdzok E. J., Billing P. Erythromycin uptake and accumulation by human polymorphonuclear leukocytes and efficacy of erythromycin in killing ingested Legionella pneumophila. J Infect Dis. 1984 May;149(5):714–718. doi: 10.1093/infdis/149.5.714. [DOI] [PubMed] [Google Scholar]
  25. Nowicki M., Paucod J. C., Bornstein N., Meugnier H., Isoard P., Fleurette J. Comparative efficacy of five antibiotics on experimental airborne legionellosis in guinea-pigs. J Antimicrob Chemother. 1988 Oct;22(4):513–519. doi: 10.1093/jac/22.4.513. [DOI] [PubMed] [Google Scholar]
  26. Pohlod D. J., Saravolatz L. D. Activity of quinolones against Legionellaceae. J Antimicrob Chemother. 1986 Apr;17(4):540–541. doi: 10.1093/jac/17.4.540. [DOI] [PubMed] [Google Scholar]
  27. Pohlod D. J., Saravolatz L. D., Somerville M. M. Inhibition of Legionella pneumophila multiplication within human macrophages by fleroxacin. J Antimicrob Chemother. 1988 Oct;22 (Suppl 500):49–54. doi: 10.1093/jac/22.supplement_d.49. [DOI] [PubMed] [Google Scholar]
  28. Prokesch R. C., Hand W. L. Antibiotic entry into human polymorphonuclear leukocytes. Antimicrob Agents Chemother. 1982 Mar;21(3):373–380. doi: 10.1128/aac.21.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rajagopalan P., Dournon E., Vildé J. L., Pocidalo J. J. Direct activation of human monocyte-derived macrophages by a bacterial glycoprotein extract inhibits the intracellular multiplication of virulent Legionella pneumophila serogroup 1. Infect Immun. 1987 Sep;55(9):2234–2239. doi: 10.1128/iai.55.9.2234-2239.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ristroph J. D., Hedlund K. W., Allen R. G. Liquid medium for growth of Legionella pneumophila. J Clin Microbiol. 1980 Jan;11(1):19–21. doi: 10.1128/jcm.11.1.19-21.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Saito A., Sawatari K., Fukuda Y., Nagasawa M., Koga H., Tomonaga A., Nakazato H., Fujita K., Shigeno Y., Suzuyama Y. Susceptibility of Legionella pneumophila to ofloxacin in vitro and in experimental Legionella pneumonia in guinea pigs. Antimicrob Agents Chemother. 1985 Jul;28(1):15–20. doi: 10.1128/aac.28.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vildé J. L., Dournon E., Rajagopalan P. Inhibition of Legionella pneumophila multiplication within human macrophages by antimicrobial agents. Antimicrob Agents Chemother. 1986 Nov;30(5):743–748. doi: 10.1128/aac.30.5.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wolfson J. S., Hooper D. C. Fluoroquinolone antimicrobial agents. Clin Microbiol Rev. 1989 Oct;2(4):378–424. doi: 10.1128/cmr.2.4.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yoshida S., Mizuguchi Y. Antibiotic susceptibility of Legionella pneumophia Philadelphia-1 in cultured guinea-pig peritoneal macrophages. J Gen Microbiol. 1984 Apr;130(4):901–906. doi: 10.1099/00221287-130-4-901. [DOI] [PubMed] [Google Scholar]

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