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Archives of Disease in Childhood. Fetal and Neonatal Edition logoLink to Archives of Disease in Childhood. Fetal and Neonatal Edition
. 1998 Mar;78(2):F125–F128. doi: 10.1136/fn.78.2.f125

Complement and contact activation in term neonates after fetal acidosis

J Sonntag, M Wagner, E Strauss, M Obladen
PMCID: PMC1720767  PMID: 9577283

Abstract

AIMS—To evaluate complement and contact activation after fetal acidosis.
METHODS—Fifteen term neonates with hypoxic-ischaemic encephalopathy after umbilical arterial pH < 7.10 were compared with 15 healthy neonates with umbilical arterial pH > 7.20. Determinations of the complement function and C1-inhibitor activity were performed as kinetic tests 22-28 hours after birth. C1q, C1-inhibitor, and factor B concentrations were determined by radial immunodiffusion and those of C3a, C5a, and factor XIIa by enzyme immunoabsorbent assay.
RESULTS—Median complement function (46 vs 73 %), C1q (4.3 vs 9.1 mg/dl), and factor B (5.2 vs 7.7 mg/dl) decreased after fetal acidosis. The activated split products C3a (260 vs 185 µg/l), C5a (5.0 vs 0.6 µg/l), and factor XIIa (3.2 vs 1.3 µg/l) increased in the neonates after fetal acidosis. No differences were found in the concentration and activity of C1-inhibitor.
CONCLUSIONS—Complement and contact activation occurred in the newborns with hypoxic-ischaemic encephalopathy. Activation of these systems generates mediators which can trigger inflammation and tissue injury.



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Selected References

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  1. Buerke M., Murohara T., Lefer A. M. Cardioprotective effects of a C1 esterase inhibitor in myocardial ischemia and reperfusion. Circulation. 1995 Jan 15;91(2):393–402. doi: 10.1161/01.cir.91.2.393. [DOI] [PubMed] [Google Scholar]
  2. Burger R., Zilow G., Bader A., Friedlein A., Naser W. The C terminus of the anaphylatoxin C3a generated upon complement activation represents a neoantigenic determinant with diagnostic potential. J Immunol. 1988 Jul 15;141(2):553–558. [PubMed] [Google Scholar]
  3. Chenoweth D. E., Cooper S. W., Hugli T. E., Stewart R. W., Blackstone E. H., Kirklin J. W. Complement activation during cardiopulmonary bypass: evidence for generation of C3a and C5a anaphylatoxins. N Engl J Med. 1981 Feb 26;304(9):497–503. doi: 10.1056/NEJM198102263040901. [DOI] [PubMed] [Google Scholar]
  4. Chávez-Cartaya R. E., DeSola G. P., Wright L., Jamieson N. V., White D. J. Regulation of the complement cascade by soluble complement receptor type 1. Protective effect in experimental liver ischemia and reperfusion. Transplantation. 1995 Apr 15;59(7):1047–1052. doi: 10.1097/00007890-199504150-00023. [DOI] [PubMed] [Google Scholar]
  5. Finer N. N., Robertson C. M., Peters K. L., Coward J. H. Factors affecting outcome in hypoxic-ischemic encephalopathy in term infants. Am J Dis Child. 1983 Jan;137(1):21–25. doi: 10.1001/archpedi.1983.02140270017006. [DOI] [PubMed] [Google Scholar]
  6. Ford R. P., Esnouf M. P., Burgess A. I., Sarphie A. An enzyme-linked immunosorbent assay (ELISA) for the measurement of activated factor XII (Hageman factor) in human plasma. J Immunoassay. 1996 May;17(2):119–131. doi: 10.1080/01971529608005783. [DOI] [PubMed] [Google Scholar]
  7. Giclas P. C., Pinckard R. N., Olson M. S. In vitro activation of complement by isolated human heart subcellular membranes. J Immunol. 1979 Jan;122(1):146–151. [PubMed] [Google Scholar]
  8. Homeister J. W., Lucchesi B. R. Complement activation and inhibition in myocardial ischemia and reperfusion injury. Annu Rev Pharmacol Toxicol. 1994;34:17–40. doi: 10.1146/annurev.pa.34.040194.000313. [DOI] [PubMed] [Google Scholar]
  9. Kjellmer I. Mechanisms of perinatal brain damage. Ann Med. 1991 Dec;23(6):675–679. doi: 10.3109/07853899109148102. [DOI] [PubMed] [Google Scholar]
  10. Klos A., Ihrig V., Messner M., Grabbe J., Bitter-Suermann D. Detection of native human complement components C3 and C5 and their primary activation peptides C3a and C5a (anaphylatoxic peptides) by ELISAs with monoclonal antibodies. J Immunol Methods. 1988 Jul 22;111(2):241–252. doi: 10.1016/0022-1759(88)90133-0. [DOI] [PubMed] [Google Scholar]
  11. Kolde H. J., Deubel R. Development of a rapid kinetic assay for the function of the classical pathway of the complement system and for C2 and C4. J Clin Lab Immunol. 1986 Dec;21(4):201–207. [PubMed] [Google Scholar]
  12. Lucchesi B. R. Complement, neutrophils and free radicals: mediators of reperfusion injury. Arzneimittelforschung. 1994 Mar;44(3A):420–432. [PubMed] [Google Scholar]
  13. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  14. Marrin M., Paes B. A. Birth asphyxia: does the Apgar score have diagnostic value? Obstet Gynecol. 1988 Jul;72(1):120–123. [PubMed] [Google Scholar]
  15. Morgan B. P. Physiology and pathophysiology of complement: progress and trends. Crit Rev Clin Lab Sci. 1995;32(3):265–298. doi: 10.3109/10408369509084686. [DOI] [PubMed] [Google Scholar]
  16. Murdoch J., Hall R. Brain protection: physiological and pharmacological considerations. Part I: The physiology of brain injury. Can J Anaesth. 1990 Sep;37(6):663–671. doi: 10.1007/BF03006487. [DOI] [PubMed] [Google Scholar]
  17. Pietz J., Guttenberg N., Gluck L. Hypoxanthine: a marker for asphyxia. Obstet Gynecol. 1988 Nov;72(5):762–766. [PubMed] [Google Scholar]
  18. Robertson C. M., Finer N. N., Grace M. G. School performance of survivors of neonatal encephalopathy associated with birth asphyxia at term. J Pediatr. 1989 May;114(5):753–760. doi: 10.1016/s0022-3476(89)80132-5. [DOI] [PubMed] [Google Scholar]
  19. Rossen R. D., Michael L. H., Kagiyama A., Savage H. E., Hanson G., Reisberg M. A., Moake J. N., Kim S. H., Self D., Weakley S. Mechanism of complement activation after coronary artery occlusion: evidence that myocardial ischemia in dogs causes release of constituents of myocardial subcellular origin that complex with human C1q in vivo. Circ Res. 1988 Mar;62(3):572–584. doi: 10.1161/01.res.62.3.572. [DOI] [PubMed] [Google Scholar]
  20. Saatvedt K., Lindberg H., Geiran O. R., Michelsen S., Aasen A. O., Pedersen T., Mollnes T. E. Complement activation and release of tumour necrosis factor alpha, interleukin-2, interleukin-6 and soluble tumour necrosis factor and interleukin-2 receptors during and after cardiopulmonary bypass in children. Scand J Clin Lab Invest. 1995 Feb;55(1):79–86. doi: 10.3109/00365519509075381. [DOI] [PubMed] [Google Scholar]
  21. Sarnat H. B., Sarnat M. S. Neonatal encephalopathy following fetal distress. A clinical and electroencephalographic study. Arch Neurol. 1976 Oct;33(10):696–705. doi: 10.1001/archneur.1976.00500100030012. [DOI] [PubMed] [Google Scholar]
  22. Schrod L., Frauendienst-Egger G., von Stockhausen H. B., Kirschfink M. Complement fragment C3a in plasma of asphyxiated neonates. Eur J Pediatr. 1992 Sep;151(9):688–692. doi: 10.1007/BF01957575. [DOI] [PubMed] [Google Scholar]
  23. Seghaye M. C., Duchateau J., Grabitz R. G., Nitsch G., Marcus C., Messmer B. J., von Bernuth G. Complement, leukocytes, and leukocyte elastase in full-term neonates undergoing cardiac operation. J Thorac Cardiovasc Surg. 1994 Jul;108(1):29–36. [PubMed] [Google Scholar]
  24. Suzuki S. Blood coagulation and fibrinolysis of the newborn viewed as perinatal factors. I. Blood coagulation and fibrinolysis of the newborn viewed as obstetrical factors. J Perinat Med. 1977;5(6):274–283. doi: 10.1515/jpme.1977.5.6.274. [DOI] [PubMed] [Google Scholar]
  25. Thornberg E., Thiringer K., Odeback A., Milsom I. Birth asphyxia: incidence, clinical course and outcome in a Swedish population. Acta Paediatr. 1995 Aug;84(8):927–932. doi: 10.1111/j.1651-2227.1995.tb13794.x. [DOI] [PubMed] [Google Scholar]
  26. Väkevä A., Morgan B. P., Tikkanen I., Helin K., Laurila P., Meri S. Time course of complement activation and inhibitor expression after ischemic injury of rat myocardium. Am J Pathol. 1994 Jun;144(6):1357–1368. [PMC free article] [PubMed] [Google Scholar]

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