Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1988 Mar;32(3):397–399. doi: 10.1128/aac.32.3.397

Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in beta-lactamase characterization of 114 resistant strains.

M Ouellette 1, G C Paul 1, A M Philippon 1, P H Roy 1
PMCID: PMC172184  PMID: 3259123

Abstract

Oligonucleotide probes specific for detection of the TEM-1 and OXA-1 beta-lactamase genes were compared with isoelectric focusing in 114 gram-negative beta-lactamase-producing strains representing at least 16 species. Correlations of 96 and 100% with isoelectric points were found for the TEM-1 and OXA-1 probes, respectively.

Full text

PDF
397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambler R. P. The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321–331. doi: 10.1098/rstb.1980.0049. [DOI] [PubMed] [Google Scholar]
  2. Chu B. C., Orgel L. E. Detection of specific DNA sequences with short biotin-labeled probes. DNA. 1985 Aug;4(4):327–331. doi: 10.1089/dna.1985.4.327. [DOI] [PubMed] [Google Scholar]
  3. Cooksey R. C., Clark N. C., Thornsberry C. A gene probe for TEM type beta-lactamases. Antimicrob Agents Chemother. 1985 Jul;28(1):154–156. doi: 10.1128/aac.28.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jouvenot M., Bonin P., Michel-Briand Y. Frequency of beta-lactamases that are markedly active against carbenicillin in the Pseudomonas aeruginosa strains isolated in a medical school hospital. J Antimicrob Chemother. 1983 Nov;12(5):451–458. doi: 10.1093/jac/12.5.451. [DOI] [PubMed] [Google Scholar]
  5. Jouvenot M., Deschaseaux M. L., Royez M., Mougin C., Cooksey R. C., Michel-Briand Y., Adessi G. L. Molecular hybridization versus isoelectric focusing to determine TEM-type beta-lactamases in gram-negative bacteria. Antimicrob Agents Chemother. 1987 Feb;31(2):300–305. doi: 10.1128/aac.31.2.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kliebe C., Nies B. A., Meyer J. F., Tolxdorff-Neutzling R. M., Wiedemann B. Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother. 1985 Aug;28(2):302–307. doi: 10.1128/aac.28.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Levesque R. C., Medeiros A. A., Jacoby G. A. Molecular cloning and DNA homology of plasmid-mediated beta-lactamase genes. Mol Gen Genet. 1987 Feb;206(2):252–258. doi: 10.1007/BF00333581. [DOI] [PubMed] [Google Scholar]
  8. Martel A. Y., Gosselin P., Ouellette M., Roy P. H., Bergeron M. G. Isolation and molecular characterization of beta-lactamase-producing Haemophilus parainfluenzae from the genital tract. Antimicrob Agents Chemother. 1987 Jun;31(6):966–968. doi: 10.1128/aac.31.6.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mathew A., Harris A. M., Marshall M. J., Ross G. W. The use of analytical isoelectric focusing for detection and identification of beta-lactamases. J Gen Microbiol. 1975 May;88(1):169–178. doi: 10.1099/00221287-88-1-169. [DOI] [PubMed] [Google Scholar]
  10. Matthew M., Hedges R. W., Smith J. T. Types of beta-lactamase determined by plasmids in gram-negative bacteria. J Bacteriol. 1979 Jun;138(3):657–662. doi: 10.1128/jb.138.3.657-662.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Medeiros A. A. Beta-lactamases. Br Med Bull. 1984 Jan;40(1):18–27. doi: 10.1093/oxfordjournals.bmb.a071942. [DOI] [PubMed] [Google Scholar]
  12. Medeiros A. A., Cohenford M., Jacoby G. A. Five novel plasmid-determined beta-lactamases. Antimicrob Agents Chemother. 1985 May;27(5):715–719. doi: 10.1128/aac.27.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ouellette M., Rossi J. J., Bazin R., Roy P. H. Oligonucleotide probes for the detection of TEM-1 and TEM-2 beta-lactamase genes and their transposons. Can J Microbiol. 1987 Mar;33(3):205–211. doi: 10.1139/m87-035. [DOI] [PubMed] [Google Scholar]
  14. Ouellette M., Roy P. H. Analysis by using DNA probes of the OXA-1 beta-lactamase gene and its transposon. Antimicrob Agents Chemother. 1986 Jul;30(1):46–51. doi: 10.1128/aac.30.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Philippon A. M., Paul G. C., Jacoby G. A. New plasmid-mediated oxacillin-hydrolyzing beta-lactamase in Pseudomonas aeruginosa. J Antimicrob Chemother. 1986 Apr;17(4):415–422. doi: 10.1093/jac/17.4.415. [DOI] [PubMed] [Google Scholar]
  16. Philippon A. M., Paul G. C., Jacoby G. A. Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1983 Sep;24(3):362–369. doi: 10.1128/aac.24.3.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Philippon A., Thabaut A., Meyran M., Nevot P. Distribution des bêta-lactamases constitutives chez Pseudomonas aeruginosa. Presse Med. 1984 Mar 29;13(13):772–776. [PubMed] [Google Scholar]
  18. Tenover F. C. Studies of antimicrobial resistance genes using DNA probes. Antimicrob Agents Chemother. 1986 May;29(5):721–725. doi: 10.1128/aac.29.5.721. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES