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. 1988 Apr;32(4):488–491. doi: 10.1128/aac.32.4.488

Host range of the conjugative 25.2-megadalton tetracycline resistance plasmid from Neisseria gonorrhoeae and related species.

M C Roberts 1, J S Knapp 1
PMCID: PMC172207  PMID: 3132092

Abstract

High-level tetracycline resistance in strains of Neisseria gonorrhoeae, Neisseria meningitidis, Kingella denitrificans, and Eikenella corrodens has recently been described. The resistance in each species is due to the acquisition of 25.2-megadalton conjugative plasmids that carry the tetracycline resistance determinant TetM. We examined the ability of commensal Neisseria species to serve as recipients in conjugation for these new plasmids. Most of the recipients (n = 21) tested had detectable conjugation frequencies (greater than 10(-9] with one or more of the donor strains. Transfer was not detected in Branhamella catarrhalis. Transconjugants were able to maintain the plasmids and act as donors in subsequent matings.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown J. T., Roberts M. C. Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain. Antimicrob Agents Chemother. 1987 Nov;31(11):1852–1854. doi: 10.1128/aac.31.11.1852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burdett V., Inamine J., Rajagopalan S. Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol. 1982 Mar;149(3):995–1004. doi: 10.1128/jb.149.3.995-1004.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenstein B. I., Sox T., Biswas G., Blackman E., Sparling P. F. Conjugal transfer of the gonococcal penicillinase plasmid. Science. 1977 Mar 11;195(4282):998–1000. doi: 10.1126/science.402693. [DOI] [PubMed] [Google Scholar]
  5. Flett F., Humphreys G. O., Saunders J. R. Intraspecific and intergeneric mobilization of non-conjugative resistance plasmids by a 24.5 megadalton conjugative plasmid of Neisseria gonorrhoeae. J Gen Microbiol. 1981 Jul;125(1):123–129. doi: 10.1099/00221287-125-1-123. [DOI] [PubMed] [Google Scholar]
  6. Genco C. A., Knapp J. S., Clark V. L. Conjugation of plasmids of Neisseria gonorrhoeae to other Neisseria species: potential reservoirs for the beta-lactamase plasmid. J Infect Dis. 1984 Sep;150(3):397–401. doi: 10.1093/infdis/150.3.397. [DOI] [PubMed] [Google Scholar]
  7. Ikeda F., Tsuji A., Kaneko Y., Nishida M., Goto S. Conjugal transfer of beta-lactamase-producing plasmids of Neisseria gonorrhoeae to Neisseria meningitidis. Microbiol Immunol. 1986;30(8):737–742. doi: 10.1111/j.1348-0421.1986.tb03000.x. [DOI] [PubMed] [Google Scholar]
  8. Kirven L. A., Thornsberry C. Transfer of beta-lactamase genes of Neisseria gonorrhoeae by conjugation. Antimicrob Agents Chemother. 1977 Jun;11(6):1004–1006. doi: 10.1128/aac.11.6.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knapp J. S., Zenilman J. M., Biddle J. W., Perkins G. H., DeWitt W. E., Thomas M. L., Johnson S. R., Morse S. A. Frequency and distribution in the United States of strains of Neisseria gonorrhoeae with plasmid-mediated, high-level resistance to tetracycline. J Infect Dis. 1987 Apr;155(4):819–822. doi: 10.1093/infdis/155.4.819. [DOI] [PubMed] [Google Scholar]
  10. Morse S. A., Johnson S. R., Biddle J. W., Roberts M. C. High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother. 1986 Nov;30(5):664–670. doi: 10.1128/aac.30.5.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roberts M. C., Kenny G. E. Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum. Antimicrob Agents Chemother. 1986 Feb;29(2):350–352. doi: 10.1128/aac.29.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roberts M. C., Koutsky L. A., Holmes K. K., LeBlanc D. J., Kenny G. E. Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences. Antimicrob Agents Chemother. 1985 Jul;28(1):141–143. doi: 10.1128/aac.28.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roberts M., Elwell L. P., Falkow S. Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol. 1977 Aug;131(2):557–563. doi: 10.1128/jb.131.2.557-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts M., Falkow S. Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature. 1977 Apr 14;266(5603):630–631. doi: 10.1038/266630a0. [DOI] [PubMed] [Google Scholar]
  15. Roberts M., Piot P., Falkow S. The ecology of gonococcal plasmids. J Gen Microbiol. 1979 Oct;114(2):491–494. doi: 10.1099/00221287-114-2-491. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Stiffler P. W., Lerner S. A., Bohnhoff M., Morello J. A. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae. J Bacteriol. 1975 Jun;122(3):1293–1300. doi: 10.1128/jb.122.3.1293-1300.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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