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. 1997 Jul;81(7):595–598. doi: 10.1136/bjo.81.7.595

Human papilloma virus in neoplastic and non-neoplastic conditions of the external eye

Z Karcioglu 1, T Issa 1
PMCID: PMC1722242  PMID: 9290377

Abstract

AIM—Human papilloma virus (HPV) types 16 and 18 have been associated with neoplastic conditions of the conjunctiva. However, the presence of this virus has not been reported in non-neoplastic disorders of the external eye nor has it been studied in normal conjunctival tissues.
METHODS—Ninety six paraffin embedded tissue specimens with neoplastic and non-neoplastic lesions and 19 conjunctiva samples free from overt disease were studied for HPV types 16 and 18 positivity with the polymerase chain reaction.
RESULTS—HPV types 16 and 18 DNA were identified in 57% of in situ squamous cell carcinoma, in 55% of invasive squamous cell carcinoma, in 20% of climatic droplet keratopathy, in 35% of scarred corneas, and in 32% of normal conjunctival tissue obtained during routine cataract extractions.
CONCLUSION—These findings indicate that HPV types 16 and 18 are detectable with the polymerase chain reaction not only in epithelial neoplasms of the ocular mucous membrane but also in non-neoplastic lesions as well as in apparently healthy conjunctiva.



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Figure 1  .

Figure 1  

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Detection of β globin gene. Ethidium bromide stained 2% agarose gel showing β globin gene amplification (110 bp product) following PCR amplification using PCO3/PCO4 primer and 1 µg of DNA as a template from the following samples: lane 1, positive control (normal blood lymphocytes); lane 2, invasive squamous cell carcinoma (SCC); lane 3, in situ SCC; lane 4, corneal scar; lane 5, conjunctiva; lane 6, negative control (no DNA); M, GelMarker; M1, PhiX 174 RF Hae III marker.

Figure 2  .

Figure 2  

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First PCR amplification of HPV DNA: consensus primer for E6 ORF of HPV 16 and 18 was used as outer primer along with 1 µg of DNA as a template (307 bp product) from the following samples: lane 1, positive control for HPV 16 (CaSki cell DNA); lane 2, positive control for HPV 18 (HeLa cell DNA); lane 3, conjunctiva; lane 4, invasive squamous cell carcinoma (SCC); lane 5, in situ SCC; lane 6, negative control (no DNA); M, GelMarker; M1, PhiX 174 RF Hae III marker.

Figure 3  .

Figure 3  

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Detection of HPV 16 DNA. HPV 16 specific inner primer was used along with 2 µl of the first PCR reaction mixture as a template. (A) Analysis of the amplification DNA products on 2% agarose gel stained with ethidium bromide (124 bp product). (B) Southern blot analysis of the amplification DNA products hybridised with non-radioactive labelled HPV 16 specific oligonucleotide probe for the following samples: lane 1, positive control (CaSki cell DNA); lane 2, conjunctiva; lane 3, conjunctiva; lane 4, invasive squamous cell carcinoma (SCC); lane 5, in situ SCC; lane 6, negative control (no DNA); M, GelMarker; M1, PhiX 174 RF Hae III marker.

Figure 4  .

Figure 4  

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Detection of HPV 18 DNA. HPV 18 specific inner primer was used along with 2 µl of the first PCR reaction mixture as a template. (A) Analysis of the amplification DNA products on 2% agarose gel stained with ethidium bromide (188 bp product). (B) Southern blot of the amplification products hybridised with non-radioactive labelled HPV 18 specific oligonucleotide probe for the following samples: lane 1, positive control for HPV 16 (HeLa cell DNA); lane 2, conjunctiva; lane 3, invasive squamous cell carcinoma (SCC); lane 4, invasive SCC; lane 5, in situ SCC; lane 6, negative control (no DNA); M, GelMarker; M1, PhiX 174 RF Hae III marker.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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