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. 1988 Apr;32(4):595–597. doi: 10.1128/aac.32.4.595

Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.

S A Brown 1, K Sugimoto 1, G G Smith 1, F B Garry 1
PMCID: PMC172229  PMID: 3377470

Abstract

Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).

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Selected References

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