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. 1998 Apr;82(4):382–388. doi: 10.1136/bjo.82.4.382

Donor organ cultured corneal tissue selection before penetrating keratoplasty

V Borderie 1, S Scheer 1, O Touzeau 1, F Vedie 1, S Carvajal-Gonzalez 1, L Laroche 1
PMCID: PMC1722561  PMID: 9640185

Abstract

AIMS—Donor organ cultured corneal tissue selection before penetrating keratoplasty is carried out by taking into account different variables. The objective was to identify preoperative variables which are significantly and independently associated with transplant outcome and should effectively be taken into account before transplantation.
METHODS—231 consecutive penetrating keratoplasties were prospectively studied using organ cultured tissue. Morphometric analysis of the donor corneal endothelium was performed before transplantation. Graft survival and endothelial cell density, during the second year following transplantation, were studied both at a univariate and multivariate level.
RESULTS—Recipient age, recipient rejection status, and preoperative diagnosis significantly influenced graft survival. Graft survival was higher when using corneal tissue from donors older than 80 years. Postoperative endothelial density decreased with preservation time and coefficient of variation after preservation. It increased with endothelial cell density after preservation and deswelling time, and correlated with preoperative diagnosis.
CONCLUSION—Organ cultured corneas with endothelial cell density after preservation <2000 cells/mm2, and high coefficient of variation, may be discarded before transplantation. Corneas should be preserved for less than 3 weeks, and allowed to deswell before transplantation for 2 or 3 days rather than 1 day.

 Keywords: corneal transplantation; endothelium; graft survival; morphometry

Full Text

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Figure 1  .

Figure 1  

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Overall graft survival (Kaplan-Meier method) of 231 consecutive penetrating keratoplasties.

Figure 2  .

Figure 2  

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Influence of donor age on graft survival (Kaplan-Meier method). Log rank: p = 0.024. Multivariate Cox hazards model: p = 0.005. 

Figure 3  .

Figure 3  

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Influence of recipient age on graft survival (Kaplan-Meier method). Log rank: p = 0.049. Multivariate Cox hazards model: p = 0.05. 

Figure 4  .

Figure 4  

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Influence of recipient rejection status on graft survival (Kaplan-Meier method). Log rank: p < 0.0001. Multivariate Cox hazards model: p = 0.01. 

Figure 5  .

Figure 5  

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Influence of preoperative diagnosis on graft survival (Kaplan-Meier method). Log rank: p < 0.0001. Multivariate Cox hazards model: p = 0.005. 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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