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. 1998 Jun;82(6):695–699. doi: 10.1136/bjo.82.6.695

Interferon gamma immunoreactivity in iris nerve fibres during endotoxin induced uveitis in the rat

P Yang 1, A F de Vos 1, A Kijlstra 1
PMCID: PMC1722634  PMID: 9797675

Abstract

AIMS—Previous studies have implied that interferon gamma (IFN-γ) is involved in the pathogenesis of endotoxin induced uveitis (EIU) in the rat. This study investigated the source of IFN-γ in the iris during EIU.
METHODS—Whole mounts of iris were isolated from Lewis rats before and at different times (from 4 hours to 14 days) after foot pad injection of 200 µg Salmonella typhimurium lipopolysaccharide (LPS). Immunohistological analysis was performed using monoclonal antibodies (mAbs) specific to rat IFN-γ (DB12 and DB13). mAbs specific to monocytes, macrophages, and dendritic cells and MHC class II were used to asses the inflammatory response in the eye (ED-1, ED-2, and OX-6). An antibody specific to neurofilaments (2H3) was used to stain nerve fibres in the normal iris.
RESULTS—LPS administration induced acute intraocular inflammation, characterised by a massive infiltration of monocytes/macrophages and increased numbers of MHC class II positive cells in the iris. IFN-γ immunoreactive cells were not detected in iris whole mounts of control rats. Strikingly, IFN-γ immunoreactivity was found in fibres from 4 hours until 10 days after LPS injection, with the most intense staining at 48-72 hours. Other DB12 or DB13 positive cells were not detected in the iris. The pattern of DB12 and DB13 staining in the inflamed iris was similar to the 2H3 staining of neurons in the iris of control rats.
CONCLUSION—These results show that systemic LPS administration induces IFN-γ immunoreactivity in iris fibres and suggest that iris nerve fibres may be a source of IFN-γ during EIU. The IFN-γ immunoreactive material in the iris nerve fibres may be identical to neuronal IFN-γ.

 Keywords: endotoxin induced uveitis; cytokines; interferon gamma; rat

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Figure 1  .

Figure 1  

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Immunohistochemical staining of iris whole mounts with mAbs DB12 (A and B) and DB13 (C and D). Iris whole mounts used were isolated from a control rat (A) and at 24 hours (B), 48 hours (C), and 72 hours (D) after LPS injection. Original magnifications, A ×250; B ×250; C ×63; and D ×100.

Figure 2  .

Figure 2  

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Immunohistochemical staining of an iris whole mount isolated from a control rat with mAbs 2H3. Original magnification, ×100.

Selected References

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