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. 1999 Oct;83(10):1168–1171. doi: 10.1136/bjo.83.10.1168

Cell and protein adhesion studies in glaucoma drainage device development

A The
PMCID: PMC1722829  PMID: 10502580

Abstract

AIM—To examine in vitro whether phosphorylcholine coating of poly(methylmethacrylate) can reduce the adhesion of fibrinogen, fibrin, human scleral fibroblast and macrophage compared with current biomaterials used in the construction of glaucoma drainage devices.
METHODS—Sample discs (n=6) of poly(methylmethacrylate), silicone, polypropylene, PTFE, and phosphorylcholine coated poly(methylmethacrylate) were seeded with fibrinogen, fibrin, fibroblast, and macrophages and incubated for variable lengths of time. The quantification was performed using radioactivity, spectrophotometry, ATP dependent luminometry, and immunohistochemistry respectively.
RESULTS—Fibrinogen and fibrin adhesion to phosphorylcholine coated poly(methylmethacrylate) were significantly lower than PMMA (p=0.004). Phosphorylcholine coating of poly(methylmethacrylate) also significantly reduced the adhesion of human scleral fibroblast (p=0.002) and macrophage (p=0.01) compared with PMMA. All the other biomaterials showed either similar or insignificantly different levels of adhesion to all the proteins and cells tested compared with PMMA.
CONCLUSION—Phosphorylcholine coating is a new material technology that offers considerable promise in the field of glaucoma drainage device development.



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Figure 1  .

Figure 1  

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Adsorption of iodine-125 labelled fibrinogen to biomaterials after 2 hours' incubation in PBS.

Figure 2  .

Figure 2  

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Adhesion of fibrin to biomaterials from fresh plasma after 2 hours' incubation.

Figure 3  .

Figure 3  

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In vitro adhesion of human scleral fibroblast to biomaterials.

Figure 4  .

Figure 4  

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In vitro adhesion of macrophage to biomaterials.

Selected References

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