Skip to main content
PMC home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 2000 Jun;84(6):648–650. doi: 10.1136/bjo.84.6.648

Conjunctival epithelium expression of HLA-DR in glaucoma patients and its influence on the outcome of filtration surgery

A Ihan 1, B Cvenkel 1
PMCID: PMC1723488  PMID: 10837395

Abstract

AIMS—To analyse the expression of HLA-DR on conjunctival epithelial cells in patients with glaucoma taking topical antiglaucoma therapy.
METHODS—10 patients taking no topical medication and 30 patients with uncontrolled glaucoma taking medical therapy participated in the study. The specimens were obtained by impression cytology preoperatively, 3 months, and 6 months after filtration surgery. The expression of HLA-DR on T lymphocytes and epithelial cells was analysed by flow cytometry.
RESULTS—A significant increase in HLA-DR on epithelial cells was found preoperatively in patients with glaucoma. A significant increased expression of HLA-DR on epithelial cells was detected 3 months and 6 months after surgery.
CONCLUSIONS—The increased expression of HLA-DR on conjunctival epithelial cells still present 6 months after surgery indicates the increased ability of epithelial cells to induce immune inflammation with subsequent fibrosis.



Full Text

The Full Text of this article is available as a PDF (104.2 KB).

Figure 1  .

Figure 1  

Open in a new tab

Cells isolated from imprint of ocular surface were incubated with a combination of CD3, anticytokeratin antibodies, and anti-HLA-DR antibodies. Cells were gated on a forward scatter cell size (FCS) versus side scatter (SSC) plot. CD3 positive cells and cytokeratin positive cells were separately analysed for the anti-HLA-DR molecule expression. (A) Epithelial cells were gated on a FSC versus SSC plot. A gate was set in to obtain HLA-DR positive epithelial cells. (B) Double gated cells were analysed for the HLA-DR molecule expression. (C) Epithelial cells with higher mean fluorescence intensities. (D) Epithelial cells with weaker mean fluorescence intensities.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baudouin C., Garcher C., Haouat N., Bron A., Gastaud P. Expression of inflammatory membrane markers by conjunctival cells in chronically treated patients with glaucoma. Ophthalmology. 1994 Mar;101(3):454–460. doi: 10.1016/s0161-6420(94)31322-4. [DOI] [PubMed] [Google Scholar]
  2. Broadway D. C., Grierson I., O'Brien C., Hitchings R. A. Adverse effects of topical antiglaucoma medication. II. The outcome of filtration surgery. Arch Ophthalmol. 1994 Nov;112(11):1446–1454. doi: 10.1001/archopht.1994.01090230060021. [DOI] [PubMed] [Google Scholar]
  3. Cairns J. E. Trabeculectomy. Preliminary report of a new method. Am J Ophthalmol. 1968 Oct;66(4):673–679. [PubMed] [Google Scholar]
  4. Chen T. C., Wilensky J. T., Viana M. A. Long-term follow-up of initially successful trabeculectomy. Ophthalmology. 1997 Jul;104(7):1120–1125. doi: 10.1016/s0161-6420(97)30174-2. [DOI] [PubMed] [Google Scholar]
  5. Hitchings R. A., Grierson I. Clinico pathological correlation in eyes with failed fistulizing surgery. Trans Ophthalmol Soc U K. 1983;103(Pt 1):84–88. [PubMed] [Google Scholar]
  6. Inaba Z. Long-term results of trabeculectomy in the Japanese: an analysis by life-table method. Jpn J Ophthalmol. 1982;26(4):361–373. [PubMed] [Google Scholar]
  7. Lavin M. J., Wormald R. P., Migdal C. S., Hitchings R. A. The influence of prior therapy on the success of trabeculectomy. Arch Ophthalmol. 1990 Nov;108(11):1543–1548. doi: 10.1001/archopht.1990.01070130045027. [DOI] [PubMed] [Google Scholar]
  8. Skuta G. L., Parrish R. K., 2nd Wound healing in glaucoma filtering surgery. Surv Ophthalmol. 1987 Nov-Dec;32(3):149–170. doi: 10.1016/0039-6257(87)90091-9. [DOI] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES