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. 2000 Jul;84(7):727–731. doi: 10.1136/bjo.84.7.727

Increased polymorphonuclear leucocyte rigidity in HIV infected individuals

A Tufail 1, G Holland 1, T Fisher 1, W Cumberland 1, H Meiselman 1
PMCID: PMC1723534  PMID: 10873983

Abstract

AIM—Individuals with human immunodeficiency virus (HIV) infection were evaluated for evidence of abnormal polymorphonuclear leucocyte (PMN) rigidity, which can alter capillary blood flow.
METHODS—The transit time of individual PMN through 8 µm pores in a cell transit analyser was used as a measure of cell rigidity. PMN transit time was compared between HIV infected individuals (n=45) with and without CMV retinitis and HIV negative controls (n=17).
RESULTS—Transit times were longer for PMN from HIV infected individuals than for PMN from controls (p<0.001). PMN from HIV infected individuals with CMV retinitis (n=13) had longer transit times than PMN from those without CMV retinitis (n=32, p<0.001). Transit times were longer in HIV infected individuals with lower CD4+ T lymphocyte counts (p<0.001). Regression analysis indicated that the relation between transit times and the presence of CMV retinitis could not be explained solely on the basis of low CD4+ T lymphocytes. In HIV infected individuals, mean transit time was not correlated with age, blood pressure, or serum creatinine, cholesterol, or triglycerides.
CONCLUSIONS—HIV infected individuals appear to have increased PMN rigidity, a cellular change that might be involved in the pathogenesis of HIV related retinal microvasculopathy. PMN rigidity appears to be related to severity of immune dysfunction. PMN rigidity may remain high in patients with CMV retinitis after elevations of CD4+ T lymphocyte counts that result from potent antiretroviral therapy.



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Figure 1  .

Figure 1  

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Polymorphonuclear leucocyte rigidity (expressed as in vitro transit times in a cell transit analyser) versus CD4+ T lymphocyte count in HIV infected individuals. There is a statistically significant correlation (r=−0.61, p<0.0001). Individuals without CMV retinitis are indicated with solid symbols; individuals with CMV retinitis are indicated with open symbols.

Selected References

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